Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i>
<i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>) is an opportunistic bacterium that has drawn attention due to its resistance to carbapenem antibiotics. The treatment of patients with severe infections has been challenging. Thus, silver nanoparticles (AgNPs) have been appl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/535 |
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| author | Inglid Fontoura Thaís S. Veriato Leandro J. Raniero Maiara L. Castilho |
| author_facet | Inglid Fontoura Thaís S. Veriato Leandro J. Raniero Maiara L. Castilho |
| author_sort | Inglid Fontoura |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>) is an opportunistic bacterium that has drawn attention due to its resistance to carbapenem antibiotics. The treatment of patients with severe infections has been challenging. Thus, silver nanoparticles (AgNPs) have been applied for their antimicrobial effects. This work aims to analyze the synergistic effect of the carbapenem antibiotic Imipenem with AgNPs against different susceptibility clinical profiles of <i>K. pneumoniae</i>. The silver nanoparticles were synthesized by bottom-up methodology and capped with alpha-lipoic acid. Susceptibility tests were performed using four <i>K. pneumoniae</i> strains with different susceptibility profiles to Imipenem. The strains were induced to form a biofilm for 48 h. Crystal violet and Resazurin assays were performed to determine biofilm formation and minimal inhibitory concentration, respectively. The reduction in Imipenem concentration with the association of nanoparticles was found in all strains studied in planktonic form, and the synergism between silver nanoparticles and Imipenem was demonstrated through the analysis of the fractional inhibitory concentration index. The viability percentage was reduced at rates ≥80% in the biofilm analysis, characterized by the minimal biofilm inhibitory concentration. The study’s proposed association resulted in inhibitory effects on different <i>K. pneumoniae</i> profiles, both in planktonic forms and biofilm, with peculiar behavior in the Imipenem-resistant profile. |
| first_indexed | 2024-03-11T07:01:12Z |
| format | Article |
| id | doaj.art-1ba8c0b0fcc24875874c52512761c1cd |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T07:01:12Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-1ba8c0b0fcc24875874c52512761c1cd2023-11-17T09:14:23ZengMDPI AGAntibiotics2079-63822023-03-0112353510.3390/antibiotics12030535Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i>Inglid Fontoura0Thaís S. Veriato1Leandro J. Raniero2Maiara L. Castilho3Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos 12244-000, SP, BrazilBionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos 12244-000, SP, BrazilNanosensors Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos 12244-000, SP, BrazilBionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos 12244-000, SP, Brazil<i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>) is an opportunistic bacterium that has drawn attention due to its resistance to carbapenem antibiotics. The treatment of patients with severe infections has been challenging. Thus, silver nanoparticles (AgNPs) have been applied for their antimicrobial effects. This work aims to analyze the synergistic effect of the carbapenem antibiotic Imipenem with AgNPs against different susceptibility clinical profiles of <i>K. pneumoniae</i>. The silver nanoparticles were synthesized by bottom-up methodology and capped with alpha-lipoic acid. Susceptibility tests were performed using four <i>K. pneumoniae</i> strains with different susceptibility profiles to Imipenem. The strains were induced to form a biofilm for 48 h. Crystal violet and Resazurin assays were performed to determine biofilm formation and minimal inhibitory concentration, respectively. The reduction in Imipenem concentration with the association of nanoparticles was found in all strains studied in planktonic form, and the synergism between silver nanoparticles and Imipenem was demonstrated through the analysis of the fractional inhibitory concentration index. The viability percentage was reduced at rates ≥80% in the biofilm analysis, characterized by the minimal biofilm inhibitory concentration. The study’s proposed association resulted in inhibitory effects on different <i>K. pneumoniae</i> profiles, both in planktonic forms and biofilm, with peculiar behavior in the Imipenem-resistant profile.https://www.mdpi.com/2079-6382/12/3/535silver nanoparticlesImipenem<i>Klebsiella pneumoniae</i>carbapenemssusceptibility profilesresistant bacteria |
| spellingShingle | Inglid Fontoura Thaís S. Veriato Leandro J. Raniero Maiara L. Castilho Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> Antibiotics silver nanoparticles Imipenem <i>Klebsiella pneumoniae</i> carbapenems susceptibility profiles resistant bacteria |
| title | Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> |
| title_full | Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> |
| title_fullStr | Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> |
| title_full_unstemmed | Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> |
| title_short | Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of <i>Klebsiella pneumoniae</i> |
| title_sort | analysis of capped silver nanoparticles combined with imipenem against different susceptibility profiles of i klebsiella pneumoniae i |
| topic | silver nanoparticles Imipenem <i>Klebsiella pneumoniae</i> carbapenems susceptibility profiles resistant bacteria |
| url | https://www.mdpi.com/2079-6382/12/3/535 |
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