Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria
Quorum sensing (QS) is a process that regulates gene expression based on cell density. In bacteria, QS facilitates collaboration and controls a large number of pathways, including biofilm formation and virulence factor production, which lead to lower sensitivity to antibiotics and higher toxicity in...
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| Format: | Artikel |
| Sprache: | English |
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Frontiers Media S.A.
2022-07-01
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| Schriftenreihe: | Frontiers in Microbiology |
| Schlagworte: | |
| Online Zugang: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.934235/full |
| _version_ | 1828517295289270272 |
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| author | Wouter A. G. Beenker Jelmer Hoeksma Jeroen den Hertog Jeroen den Hertog |
| author_facet | Wouter A. G. Beenker Jelmer Hoeksma Jeroen den Hertog Jeroen den Hertog |
| author_sort | Wouter A. G. Beenker |
| collection | DOAJ |
| description | Quorum sensing (QS) is a process that regulates gene expression based on cell density. In bacteria, QS facilitates collaboration and controls a large number of pathways, including biofilm formation and virulence factor production, which lead to lower sensitivity to antibiotics and higher toxicity in the host, respectively. Inhibition of QS is a promising strategy to combat bacterial infections. In this study, we tested the potential of secondary metabolites from fungi to inhibit bacterial QS using a library derived from more than ten thousand different fungal strains. We used the reporter bacterium, Chromobacterium violaceum, and identified 39 fungal strains that produced QS inhibitor activity. These strains expressed two QS inhibitors that had been described before and eight QS inhibitors that had not been described before. Further testing for QS inhibitor activity against the opportunistic pathogen Pseudomonas aeruginosa led to the identification of gregatins as an interesting family of compounds with QS inhibitor activity. Although various gregatins inhibited QS in P. aeruginosa, these gregatins did not inhibit virulence factor production and biofilm formation. We conclude that gregatins inhibit some, but not all aspects of QS. |
| first_indexed | 2024-12-11T18:39:34Z |
| format | Article |
| id | doaj.art-1ba9e96e23a84acbbf2b57c47fbd02f2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T18:39:34Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1ba9e96e23a84acbbf2b57c47fbd02f22022-12-22T00:54:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.934235934235Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative BacteriaWouter A. G. Beenker0Jelmer Hoeksma1Jeroen den Hertog2Jeroen den Hertog3Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, NetherlandsHubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, NetherlandsHubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, NetherlandsInstitute Biology Leiden, Leiden University, Leiden, NetherlandsQuorum sensing (QS) is a process that regulates gene expression based on cell density. In bacteria, QS facilitates collaboration and controls a large number of pathways, including biofilm formation and virulence factor production, which lead to lower sensitivity to antibiotics and higher toxicity in the host, respectively. Inhibition of QS is a promising strategy to combat bacterial infections. In this study, we tested the potential of secondary metabolites from fungi to inhibit bacterial QS using a library derived from more than ten thousand different fungal strains. We used the reporter bacterium, Chromobacterium violaceum, and identified 39 fungal strains that produced QS inhibitor activity. These strains expressed two QS inhibitors that had been described before and eight QS inhibitors that had not been described before. Further testing for QS inhibitor activity against the opportunistic pathogen Pseudomonas aeruginosa led to the identification of gregatins as an interesting family of compounds with QS inhibitor activity. Although various gregatins inhibited QS in P. aeruginosa, these gregatins did not inhibit virulence factor production and biofilm formation. We conclude that gregatins inhibit some, but not all aspects of QS.https://www.frontiersin.org/articles/10.3389/fmicb.2022.934235/fullquorum sensinggregatinsChromobacterium violaceumPseudomonas aeruginosaantimicrobial activityfungi |
| spellingShingle | Wouter A. G. Beenker Jelmer Hoeksma Jeroen den Hertog Jeroen den Hertog Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria Frontiers in Microbiology quorum sensing gregatins Chromobacterium violaceum Pseudomonas aeruginosa antimicrobial activity fungi |
| title | Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria |
| title_full | Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria |
| title_fullStr | Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria |
| title_full_unstemmed | Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria |
| title_short | Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria |
| title_sort | gregatins a group of related fungal secondary metabolites inhibit aspects of quorum sensing in gram negative bacteria |
| topic | quorum sensing gregatins Chromobacterium violaceum Pseudomonas aeruginosa antimicrobial activity fungi |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.934235/full |
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