Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses
ABSTRACT Endophytic fungi form a significant part of the plant mycobiome. Defining core members is crucial to understanding the assembly mechanism of fungal endophytic communities (FECs) and identifying functionally important community members. We conducted a meta-analysis of FECs in stems of wheat...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00611-23 |
| _version_ | 1797803375428894720 |
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| author | Xiang Sun Or Sharon Amir Sharon |
| author_facet | Xiang Sun Or Sharon Amir Sharon |
| author_sort | Xiang Sun |
| collection | DOAJ |
| description | ABSTRACT Endophytic fungi form a significant part of the plant mycobiome. Defining core members is crucial to understanding the assembly mechanism of fungal endophytic communities (FECs) and identifying functionally important community members. We conducted a meta-analysis of FECs in stems of wheat and five wild cereal species and generated a landscape of the fungal endophytic assemblages in this group of plants. The analysis revealed that several Ascomycota members and basidiomycetous yeasts formed an important compartment of the FECs in these plants. We observed a weak spatial autocorrelation at the regional scale and high intrahost variations in the FECs, suggesting a space-related heterogeneity. Accordingly, we propose that the heterogeneity among subcommunities should be a criterion to define the core endophytic members. Analysis of the subcommunities and meta-communities showed that the core and noncore members had distinct roles in various assembly processes, such as stochasticity, universal dynamics, and network characteristics, within each host. The distinct features identified between the community partitions of endophytes aid in understanding the principles that govern the assembly and function of natural communities. These findings can assist in designing synthetic microbiomes. IMPORTANCE This study proposes a novel method for diagnosing core microbiotas based on prevalence of community members in a meta-community, which could be determined and supported statistically. Using this approach, the study found stratification in community assembly processes within fungal endophyte communities (FECs) in the stems of wheat and cereal-related wild species. The core and noncore partitions of the FECs exhibited certain degrees of determinism from different aspects. Further analysis revealed abundant and consistent interactions between rare taxa, which might contribute to the determinism process in noncore partitions. Despite minor differences in FEC compositions, wheat FECs showed distinct patterns in community assembly processes compared to wild species, suggesting the effects of domestication on FECs. Overall, our study provided a new approach for identifying core microbiota and provides insights into the community assembly processes within FECs in wheat and related wild species. |
| first_indexed | 2024-03-13T05:19:53Z |
| format | Article |
| id | doaj.art-1bab5aa0eb0346f6ae724700280bb418 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:19:53Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-1bab5aa0eb0346f6ae724700280bb4182023-06-15T13:18:33ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.00611-23Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other GrassesXiang Sun0Or Sharon1Amir Sharon2School of Life Sciences, Hebei University, Baoding, Hebei, ChinaSchool of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, IsraelSchool of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, IsraelABSTRACT Endophytic fungi form a significant part of the plant mycobiome. Defining core members is crucial to understanding the assembly mechanism of fungal endophytic communities (FECs) and identifying functionally important community members. We conducted a meta-analysis of FECs in stems of wheat and five wild cereal species and generated a landscape of the fungal endophytic assemblages in this group of plants. The analysis revealed that several Ascomycota members and basidiomycetous yeasts formed an important compartment of the FECs in these plants. We observed a weak spatial autocorrelation at the regional scale and high intrahost variations in the FECs, suggesting a space-related heterogeneity. Accordingly, we propose that the heterogeneity among subcommunities should be a criterion to define the core endophytic members. Analysis of the subcommunities and meta-communities showed that the core and noncore members had distinct roles in various assembly processes, such as stochasticity, universal dynamics, and network characteristics, within each host. The distinct features identified between the community partitions of endophytes aid in understanding the principles that govern the assembly and function of natural communities. These findings can assist in designing synthetic microbiomes. IMPORTANCE This study proposes a novel method for diagnosing core microbiotas based on prevalence of community members in a meta-community, which could be determined and supported statistically. Using this approach, the study found stratification in community assembly processes within fungal endophyte communities (FECs) in the stems of wheat and cereal-related wild species. The core and noncore partitions of the FECs exhibited certain degrees of determinism from different aspects. Further analysis revealed abundant and consistent interactions between rare taxa, which might contribute to the determinism process in noncore partitions. Despite minor differences in FEC compositions, wheat FECs showed distinct patterns in community assembly processes compared to wild species, suggesting the effects of domestication on FECs. Overall, our study provided a new approach for identifying core microbiota and provides insights into the community assembly processes within FECs in wheat and related wild species.https://journals.asm.org/doi/10.1128/spectrum.00611-23community assemblycore microbiomeendophytic fungiwheatwild cereals |
| spellingShingle | Xiang Sun Or Sharon Amir Sharon Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses Microbiology Spectrum community assembly core microbiome endophytic fungi wheat wild cereals |
| title | Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses |
| title_full | Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses |
| title_fullStr | Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses |
| title_full_unstemmed | Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses |
| title_short | Distinct Features Based on Partitioning of the Endophytic Fungi of Cereals and Other Grasses |
| title_sort | distinct features based on partitioning of the endophytic fungi of cereals and other grasses |
| topic | community assembly core microbiome endophytic fungi wheat wild cereals |
| url | https://journals.asm.org/doi/10.1128/spectrum.00611-23 |
| work_keys_str_mv | AT xiangsun distinctfeaturesbasedonpartitioningoftheendophyticfungiofcerealsandothergrasses AT orsharon distinctfeaturesbasedonpartitioningoftheendophyticfungiofcerealsandothergrasses AT amirsharon distinctfeaturesbasedonpartitioningoftheendophyticfungiofcerealsandothergrasses |