Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis
Semen analysis has long been used to evaluate male fertility. Recently, several sperm function tests have been developed. Of those, the sperm DNA fragmentation index (DFI), which describes the status of the sperm DNA, is thought to be a suitable parameter for evaluating male fertility. However, ther...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2022;volume=24;issue=1;spage=40;epage=44;aulast=Osaka |
| _version_ | 1818976889519210496 |
|---|---|
| author | Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto |
| author_facet | Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto |
| author_sort | Akiyoshi Osaka |
| collection | DOAJ |
| description | Semen analysis has long been used to evaluate male fertility. Recently, several sperm function tests have been developed. Of those, the sperm DNA fragmentation index (DFI), which describes the status of the sperm DNA, is thought to be a suitable parameter for evaluating male fertility. However, there have been no large-scale studies on the sperm DFI of Japanese men. Therefore, we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay (SCSA) to assess male fertility in Japan. This study enrolled 743 infertile and 20 fertile Japanese men. To evaluate reproducibility, inter- and intraobserver precision was analyzed. A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine insemination. The variability of the sperm DFI among fertile volunteers was determined. The relationship between semen parameters and the sperm DFI was assessed by Spearman's rho test. A precision analysis revealed good reproducibility of the sperm DFI. The cutoff value of sperm DNA fragmentation in infertile men was 24.0%. Semen volume had no relationship with the sperm DFI. Sperm concentration, sperm motility, total motile sperm count, and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm DFI. The median sperm DFI was smaller in fertile volunteers (7.7%) than that in infertile men (19.4%). Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis. |
| first_indexed | 2024-12-20T16:19:01Z |
| format | Article |
| id | doaj.art-1bab96038e3044a08c353ef640f05a1e |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-20T16:19:01Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-1bab96038e3044a08c353ef640f05a1e2022-12-21T19:33:41ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622022-01-01241404410.4103/aja.aja_49_21Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysisAkiyoshi OsakaHiroshi OkadaSae OnozukaTakashi TanakaToshiyuki IwahataYukihito ShimomuraYoshitomo KoboriKazutaka SaitoKouhei SugimotoSemen analysis has long been used to evaluate male fertility. Recently, several sperm function tests have been developed. Of those, the sperm DNA fragmentation index (DFI), which describes the status of the sperm DNA, is thought to be a suitable parameter for evaluating male fertility. However, there have been no large-scale studies on the sperm DFI of Japanese men. Therefore, we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay (SCSA) to assess male fertility in Japan. This study enrolled 743 infertile and 20 fertile Japanese men. To evaluate reproducibility, inter- and intraobserver precision was analyzed. A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine insemination. The variability of the sperm DFI among fertile volunteers was determined. The relationship between semen parameters and the sperm DFI was assessed by Spearman's rho test. A precision analysis revealed good reproducibility of the sperm DFI. The cutoff value of sperm DNA fragmentation in infertile men was 24.0%. Semen volume had no relationship with the sperm DFI. Sperm concentration, sperm motility, total motile sperm count, and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm DFI. The median sperm DFI was smaller in fertile volunteers (7.7%) than that in infertile men (19.4%). Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2022;volume=24;issue=1;spage=40;epage=44;aulast=Osakadna fragmentation; flow cytometry; male infertility; semen analysis; spermatozoa |
| spellingShingle | Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis Asian Journal of Andrology dna fragmentation; flow cytometry; male infertility; semen analysis; spermatozoa |
| title | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis |
| title_full | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis |
| title_fullStr | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis |
| title_full_unstemmed | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis |
| title_short | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis |
| title_sort | evaluation of the sperm dna fragmentation index in infertile japanese men by in house flow cytometric analysis |
| topic | dna fragmentation; flow cytometry; male infertility; semen analysis; spermatozoa |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2022;volume=24;issue=1;spage=40;epage=44;aulast=Osaka |
| work_keys_str_mv | AT akiyoshiosaka evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT hiroshiokada evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT saeonozuka evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT takashitanaka evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT toshiyukiiwahata evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT yukihitoshimomura evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT yoshitomokobori evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT kazutakasaito evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis AT kouheisugimoto evaluationofthespermdnafragmentationindexininfertilejapanesemenbyinhouseflowcytometricanalysis |