Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico
Eight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2022.2144951 |
| _version_ | 1811225947027800064 |
|---|---|
| author | Karen V. Pineda-Hidalgo Brianda Flores-Leyva Nancy Y. Salazar-Salas Jeanett Chávez-Ontiveros José A. Garzon-Tiznado Jorge Sánchez-López Francisco Delgado-Vargas José A. López-Valenzuela |
| author_facet | Karen V. Pineda-Hidalgo Brianda Flores-Leyva Nancy Y. Salazar-Salas Jeanett Chávez-Ontiveros José A. Garzon-Tiznado Jorge Sánchez-López Francisco Delgado-Vargas José A. López-Valenzuela |
| author_sort | Karen V. Pineda-Hidalgo |
| collection | DOAJ |
| description | Eight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and CHI) by RT-qPCR. The AC (mmol TE/kg fw; TE, Trolox Equivalents) by ABTS (5.27–11.81), DPPH (1.43–3.67), and FRAP (4.41–9.22) correlated with TP (0.53–1.20 g GAE/kg fw; GAE, Gallic Acid Equivalents), TF (1.03–2.10 g QE/kg; QE, Quercetin Equivalents), and the levels of chlorogenic and dicaffeoylquinic acids and rutin. The expression of MYB12 and MYB14 correlated with that of PAL and CHS, while CHI was only associated with MYB12. The accessions Tumbisca and Kilim showed the highest gene expression, phenolics content, and AC, suggesting they can be used in breeding programs to produce tomatoes with better AC. |
| first_indexed | 2024-04-12T09:15:43Z |
| format | Article |
| id | doaj.art-1babc05ae5be4df1b237e71e2dc0d280 |
| institution | Directory Open Access Journal |
| issn | 1947-6337 1947-6345 |
| language | English |
| last_indexed | 2024-04-12T09:15:43Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj.art-1babc05ae5be4df1b237e71e2dc0d2802022-12-22T03:38:50ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452022-12-0120137438410.1080/19476337.2022.2144951Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from MexicoKaren V. Pineda-Hidalgo0Brianda Flores-Leyva1Nancy Y. Salazar-Salas2Jeanett Chávez-Ontiveros3José A. Garzon-Tiznado4Jorge Sánchez-López5Francisco Delgado-Vargas6José A. López-Valenzuela7Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, MéxicoEight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and CHI) by RT-qPCR. The AC (mmol TE/kg fw; TE, Trolox Equivalents) by ABTS (5.27–11.81), DPPH (1.43–3.67), and FRAP (4.41–9.22) correlated with TP (0.53–1.20 g GAE/kg fw; GAE, Gallic Acid Equivalents), TF (1.03–2.10 g QE/kg; QE, Quercetin Equivalents), and the levels of chlorogenic and dicaffeoylquinic acids and rutin. The expression of MYB12 and MYB14 correlated with that of PAL and CHS, while CHI was only associated with MYB12. The accessions Tumbisca and Kilim showed the highest gene expression, phenolics content, and AC, suggesting they can be used in breeding programs to produce tomatoes with better AC.https://www.tandfonline.com/doi/10.1080/19476337.2022.2144951TomatopolyphenolsantioxidantsUPLC-MSMYB factorstomate |
| spellingShingle | Karen V. Pineda-Hidalgo Brianda Flores-Leyva Nancy Y. Salazar-Salas Jeanett Chávez-Ontiveros José A. Garzon-Tiznado Jorge Sánchez-López Francisco Delgado-Vargas José A. López-Valenzuela Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico CyTA - Journal of Food Tomato polyphenols antioxidants UPLC-MS MYB factors tomate |
| title | Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico |
| title_full | Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico |
| title_fullStr | Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico |
| title_full_unstemmed | Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico |
| title_short | Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico |
| title_sort | expression of myb transcription factors and target genes and its association with phenolic content and antioxidant activity of selected solanum lycopersicum var cerasiforme accessions from mexico |
| topic | Tomato polyphenols antioxidants UPLC-MS MYB factors tomate |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2022.2144951 |
| work_keys_str_mv | AT karenvpinedahidalgo expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT briandafloresleyva expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT nancyysalazarsalas expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT jeanettchavezontiveros expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT joseagarzontiznado expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT jorgesanchezlopez expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT franciscodelgadovargas expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico AT josealopezvalenzuela expressionofmybtranscriptionfactorsandtargetgenesanditsassociationwithphenoliccontentandantioxidantactivityofselectedsolanumlycopersicumvarcerasiformeaccessionsfrommexico |