Genome-wide expression profiling of complex regional pain syndrome.
Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3828360?pdf=render |
| _version_ | 1818294299196915712 |
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| author | Eun-Heui Jin Enji Zhang Youngkwon Ko Woo Seog Sim Dong Eon Moon Keon Jung Yoon Jang Hee Hong Won Hyung Lee |
| author_facet | Eun-Heui Jin Enji Zhang Youngkwon Ko Woo Seog Sim Dong Eon Moon Keon Jung Yoon Jang Hee Hong Won Hyung Lee |
| author_sort | Eun-Heui Jin |
| collection | DOAJ |
| description | Complex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p<0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of major histocompatibility complex class I A subtype (HLA-A29.1), matrix metalloproteinase 9 (MMP9), alanine aminopeptidase N (ANPEP), l-histidine decarboxylase (HDC), granulocyte colony-stimulating factor 3 receptor (G-CSF3R), and signal transducer and activator of transcription 3 (STAT3) genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We focused on the MMP9 gene that, by qRT-PCR, showed a statistically significant difference in expression in CRPS patients compared to controls with the highest relative fold change (4.0±1.23 times and p = 1.4×10(-4)). The up-regulation of MMP9 gene in the blood may be related to the pain progression in CRPS patients. Our findings, which offer a valuable contribution to the understanding of the differential gene expression in CRPS may help in the understanding of the pathophysiology of CRPS pain progression. |
| first_indexed | 2024-12-13T03:29:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T03:29:32Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1baf9ab3990246948929121ac6f0915b2022-12-22T00:01:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7943510.1371/journal.pone.0079435Genome-wide expression profiling of complex regional pain syndrome.Eun-Heui JinEnji ZhangYoungkwon KoWoo Seog SimDong Eon MoonKeon Jung YoonJang Hee HongWon Hyung LeeComplex regional pain syndrome (CRPS) is a chronic, progressive, and devastating pain syndrome characterized by spontaneous pain, hyperalgesia, allodynia, altered skin temperature, and motor dysfunction. Although previous gene expression profiling studies have been conducted in animal pain models, there genome-wide expression profiling in the whole blood of CRPS patients has not been reported yet. Here, we successfully identified certain pain-related genes through genome-wide expression profiling in the blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p<0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of major histocompatibility complex class I A subtype (HLA-A29.1), matrix metalloproteinase 9 (MMP9), alanine aminopeptidase N (ANPEP), l-histidine decarboxylase (HDC), granulocyte colony-stimulating factor 3 receptor (G-CSF3R), and signal transducer and activator of transcription 3 (STAT3) genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We focused on the MMP9 gene that, by qRT-PCR, showed a statistically significant difference in expression in CRPS patients compared to controls with the highest relative fold change (4.0±1.23 times and p = 1.4×10(-4)). The up-regulation of MMP9 gene in the blood may be related to the pain progression in CRPS patients. Our findings, which offer a valuable contribution to the understanding of the differential gene expression in CRPS may help in the understanding of the pathophysiology of CRPS pain progression.http://europepmc.org/articles/PMC3828360?pdf=render |
| spellingShingle | Eun-Heui Jin Enji Zhang Youngkwon Ko Woo Seog Sim Dong Eon Moon Keon Jung Yoon Jang Hee Hong Won Hyung Lee Genome-wide expression profiling of complex regional pain syndrome. PLoS ONE |
| title | Genome-wide expression profiling of complex regional pain syndrome. |
| title_full | Genome-wide expression profiling of complex regional pain syndrome. |
| title_fullStr | Genome-wide expression profiling of complex regional pain syndrome. |
| title_full_unstemmed | Genome-wide expression profiling of complex regional pain syndrome. |
| title_short | Genome-wide expression profiling of complex regional pain syndrome. |
| title_sort | genome wide expression profiling of complex regional pain syndrome |
| url | http://europepmc.org/articles/PMC3828360?pdf=render |
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