Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance
In recent years, several molecular methods have been introduced for diagnosis of Mycobacterium tuberculosis complex (MTBC), and detecting the drug resistance in clinical specimens. The FluoroType MTB (FT MTB) assay uses real-time polymerase chain reaction (PCR) to detect MTBC in clinical specimens....
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1466662 |
| _version_ | 1819163670736797696 |
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| author | Gonca Erkose Genc Dilek Satana Esra Yildirim Zayre Erturan Yildiz Yegenoglu Meltem Uzun |
| author_facet | Gonca Erkose Genc Dilek Satana Esra Yildirim Zayre Erturan Yildiz Yegenoglu Meltem Uzun |
| author_sort | Gonca Erkose Genc |
| collection | DOAJ |
| description | In recent years, several molecular methods have been introduced for diagnosis of Mycobacterium tuberculosis complex (MTBC), and detecting the drug resistance in clinical specimens. The FluoroType MTB (FT MTB) assay uses real-time polymerase chain reaction (PCR) to detect MTBC in clinical specimens. GenoType MTBDRplus is a line probe assay which detects MTBC, as well as rifampicin, and isoniazid resistance. In this study, the diagnostic performances of FT MTB and GenoType MTBDRplus were evaluated. In total, 247 specimens (124 respiratory, 123 non-respiratory) were analyzed comparing mycobacterial growth methods and FT MTB. GenoType MTBDRplus was used for the specimens positive for MTBC. In all, 23 (9.3%) of 247 specimens were positive for both the culture and FT MTB assay; therefore, the GenoType MTBDRplus assay was performed on 23 clinical specimens. The results were concordant with the drug susceptibility test results. The FT MTB assay provided quick and reliable direct detection of MTBC from the clinical specimens with high sensitivity (95.8%) and specificity (100%). Although the performance of GenoType MTBDRplus was problematic in clinical specimens with mycobacterial levels below the detection limits of the assay, it was a reliable test for cultivated specimens. |
| first_indexed | 2024-12-22T17:47:50Z |
| format | Article |
| id | doaj.art-1bb001ea272040ecb52d536ffb52cb12 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-22T17:47:50Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-1bb001ea272040ecb52d536ffb52cb122022-12-21T18:18:14ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-07-01324999100410.1080/13102818.2018.14666621466662Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistanceGonca Erkose Genc0Dilek Satana1Esra Yildirim2Zayre Erturan3Yildiz Yegenoglu4Meltem Uzun5Istanbul UniversityIstanbul UniversityIstanbul Sabahattin Zaim UniversityIstanbul UniversityIstanbul UniversityIstanbul UniversityIn recent years, several molecular methods have been introduced for diagnosis of Mycobacterium tuberculosis complex (MTBC), and detecting the drug resistance in clinical specimens. The FluoroType MTB (FT MTB) assay uses real-time polymerase chain reaction (PCR) to detect MTBC in clinical specimens. GenoType MTBDRplus is a line probe assay which detects MTBC, as well as rifampicin, and isoniazid resistance. In this study, the diagnostic performances of FT MTB and GenoType MTBDRplus were evaluated. In total, 247 specimens (124 respiratory, 123 non-respiratory) were analyzed comparing mycobacterial growth methods and FT MTB. GenoType MTBDRplus was used for the specimens positive for MTBC. In all, 23 (9.3%) of 247 specimens were positive for both the culture and FT MTB assay; therefore, the GenoType MTBDRplus assay was performed on 23 clinical specimens. The results were concordant with the drug susceptibility test results. The FT MTB assay provided quick and reliable direct detection of MTBC from the clinical specimens with high sensitivity (95.8%) and specificity (100%). Although the performance of GenoType MTBDRplus was problematic in clinical specimens with mycobacterial levels below the detection limits of the assay, it was a reliable test for cultivated specimens.http://dx.doi.org/10.1080/13102818.2018.1466662Drug resistancedrug susceptibility testingisoniazidrifampicinmycobacteriaMycobacterium tuberculosis complex |
| spellingShingle | Gonca Erkose Genc Dilek Satana Esra Yildirim Zayre Erturan Yildiz Yegenoglu Meltem Uzun Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance Biotechnology & Biotechnological Equipment Drug resistance drug susceptibility testing isoniazid rifampicin mycobacteria Mycobacterium tuberculosis complex |
| title | Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance |
| title_full | Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance |
| title_fullStr | Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance |
| title_full_unstemmed | Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance |
| title_short | Evaluation of FluoroType MTB for direct detection of Mycobacterium tuberculosis complex and GenoType MTBDRplus for determining rifampicin and isoniazid resistance |
| title_sort | evaluation of fluorotype mtb for direct detection of mycobacterium tuberculosis complex and genotype mtbdrplus for determining rifampicin and isoniazid resistance |
| topic | Drug resistance drug susceptibility testing isoniazid rifampicin mycobacteria Mycobacterium tuberculosis complex |
| url | http://dx.doi.org/10.1080/13102818.2018.1466662 |
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