Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid
Imidacloprid-induced hormesis in the form of stimulated reproduction has previously been reported in green peach aphid, Myzus persicae . Changes in gene expression accompanying this hormetic response have not been previously investigated. In this study, expression of stress response (Hsp60) , disper...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2014-07-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.2203/dose-response.13-057.Cutler |
| _version_ | 1818963956609318912 |
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| author | Murali-Mohan Ayyanath G. Christopher Cutler Cynthia D. Scott-Dupree Balakrishnan Prithiviraj Saveetha Kandasamy Kalyani Prithiviraj |
| author_facet | Murali-Mohan Ayyanath G. Christopher Cutler Cynthia D. Scott-Dupree Balakrishnan Prithiviraj Saveetha Kandasamy Kalyani Prithiviraj |
| author_sort | Murali-Mohan Ayyanath |
| collection | DOAJ |
| description | Imidacloprid-induced hormesis in the form of stimulated reproduction has previously been reported in green peach aphid, Myzus persicae . Changes in gene expression accompanying this hormetic response have not been previously investigated. In this study, expression of stress response (Hsp60) , dispersal ( OSD, TOL and ANT) , and developmental (FPPS I) genes were examined for two generations during imidacloprid-induced reproductive stimulation in M. persicae . Global DNA methylation was also measured to test the hypothesis that changes in gene expression are heritable. At hormetic concentrations, down-regulation of Hsp60 was followed by up-regulation of this gene in the subsequent generation. Likewise, expression of dispersal-related genes and FPPS I varied with concentration, life stage, and generation. These results indicate that reproductive hormesis in M. persicae is accompanied by a complex transgenerational pattern of up- and down-regulation of genes that likely reflects trade-offs in gene expression and related physiological processes during the phenotypic dose-response. Moreover, DNA methylation in second generation M. persicae occurred at higher doses than in first-generation aphids, suggesting that heritable adaptability to low doses of the stressor might have occurred. |
| first_indexed | 2024-12-20T12:53:27Z |
| format | Article |
| id | doaj.art-1bb1203ee0f2425cbe8cc9a4af7f984a |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-12-20T12:53:27Z |
| publishDate | 2014-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-1bb1203ee0f2425cbe8cc9a4af7f984a2022-12-21T19:40:07ZengSAGE PublishingDose-Response1559-32582014-07-011210.2203/dose-response.13-057.CutlerGene Expression during Imidacloprid-Induced Hormesis in Green Peach AphidMurali-Mohan Ayyanath0G. Christopher Cutler1Cynthia D. Scott-Dupree2Balakrishnan Prithiviraj3Saveetha Kandasamy4Kalyani Prithiviraj5 School of Environmental Sciences, Ontario Agricultural College, University of Guelph, Guelph, Ontario, CANADA Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, CANADA School of Environmental Sciences, Ontario Agricultural College, University of Guelph, Guelph, Ontario, CANADA Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, CANADA Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, CANADA Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, CANADAImidacloprid-induced hormesis in the form of stimulated reproduction has previously been reported in green peach aphid, Myzus persicae . Changes in gene expression accompanying this hormetic response have not been previously investigated. In this study, expression of stress response (Hsp60) , dispersal ( OSD, TOL and ANT) , and developmental (FPPS I) genes were examined for two generations during imidacloprid-induced reproductive stimulation in M. persicae . Global DNA methylation was also measured to test the hypothesis that changes in gene expression are heritable. At hormetic concentrations, down-regulation of Hsp60 was followed by up-regulation of this gene in the subsequent generation. Likewise, expression of dispersal-related genes and FPPS I varied with concentration, life stage, and generation. These results indicate that reproductive hormesis in M. persicae is accompanied by a complex transgenerational pattern of up- and down-regulation of genes that likely reflects trade-offs in gene expression and related physiological processes during the phenotypic dose-response. Moreover, DNA methylation in second generation M. persicae occurred at higher doses than in first-generation aphids, suggesting that heritable adaptability to low doses of the stressor might have occurred.https://doi.org/10.2203/dose-response.13-057.Cutler |
| spellingShingle | Murali-Mohan Ayyanath G. Christopher Cutler Cynthia D. Scott-Dupree Balakrishnan Prithiviraj Saveetha Kandasamy Kalyani Prithiviraj Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid Dose-Response |
| title | Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid |
| title_full | Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid |
| title_fullStr | Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid |
| title_full_unstemmed | Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid |
| title_short | Gene Expression during Imidacloprid-Induced Hormesis in Green Peach Aphid |
| title_sort | gene expression during imidacloprid induced hormesis in green peach aphid |
| url | https://doi.org/10.2203/dose-response.13-057.Cutler |
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