Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages
Colorectal cancer (CRC) is a growing public health challenge, featuring a multifactorial etiology and complex host–environment interactions. Recently, increasing evidence has pointed to the role of the gut microbiota in CRC development and progression. To explore the role of gut microbes in CRC, we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2365 |
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| author | Nour El Houda Mathlouthi Aicha Kriaa Leila Ammar Keskes Moez Rhimi Radhouane Gdoura |
| author_facet | Nour El Houda Mathlouthi Aicha Kriaa Leila Ammar Keskes Moez Rhimi Radhouane Gdoura |
| author_sort | Nour El Houda Mathlouthi |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a growing public health challenge, featuring a multifactorial etiology and complex host–environment interactions. Recently, increasing evidence has pointed to the role of the gut microbiota in CRC development and progression. To explore the role of gut microbes in CRC, we retrieved metagenomic data from 156 stools from the European Nucleotide Archive database and mapped them against the VFDB database for virulence factors (VFs). GO annotations of VFs and KEGG pathways were then performed to predict the microbial functions and define functional pathways enriched in the tumor-associated microbiota. Interestingly, 306 VFs were detected in the metagenomic data. We revealed the enrichment of adenomas with VFs involved in cell adhesion, whereas in the early stages of CRC they were enriched in both adhesins and isochorismatase. Advanced stages of CRC were enriched with microbial siderophores, especially enterobactin, which was significantly associated with isochorismate synthase. We highlighted higher abundances of porins and transporters involved in antibiotic resistance and the development of biofilm in advanced stages of CRC. Most VFs detected in CRC, particularly in advanced stages, were shown to be included in siderophore biosynthesis pathways. This enrichment of predicted VFs supports the key role of the gut microbiota in the disease. |
| first_indexed | 2024-03-09T16:03:56Z |
| format | Article |
| id | doaj.art-1bbdba714faf4d68b39dadb972723f07 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:03:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1bbdba714faf4d68b39dadb972723f072023-11-24T16:47:52ZengMDPI AGMicroorganisms2076-26072022-11-011012236510.3390/microorganisms10122365Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced StagesNour El Houda Mathlouthi0Aicha Kriaa1Leila Ammar Keskes2Moez Rhimi3Radhouane Gdoura4Laboratoire de Recherche Toxicologie Microbiologie Environnementale et Santé (LR17ES06), Faculté des Sciences de Sfax, Université de Sfax, Sfax 3000, TunisiaMicrobiota Interaction with Human and Animal Team (MIHA), Micalis Institute-UMR1319, Ag-roParisTech, Université Paris-Saclay, INRAE, F-78350 Jouy-en-Josas, FranceLaboratoire de Recherche de Génétique Moléculaire Humaine, Faculté de Médecine de Sfax, Avenue Majida BOULILA, Université de Sfax, Sfax 3029, TunisiaMicrobiota Interaction with Human and Animal Team (MIHA), Micalis Institute-UMR1319, Ag-roParisTech, Université Paris-Saclay, INRAE, F-78350 Jouy-en-Josas, FranceLaboratoire de Recherche Toxicologie Microbiologie Environnementale et Santé (LR17ES06), Faculté des Sciences de Sfax, Université de Sfax, Sfax 3000, TunisiaColorectal cancer (CRC) is a growing public health challenge, featuring a multifactorial etiology and complex host–environment interactions. Recently, increasing evidence has pointed to the role of the gut microbiota in CRC development and progression. To explore the role of gut microbes in CRC, we retrieved metagenomic data from 156 stools from the European Nucleotide Archive database and mapped them against the VFDB database for virulence factors (VFs). GO annotations of VFs and KEGG pathways were then performed to predict the microbial functions and define functional pathways enriched in the tumor-associated microbiota. Interestingly, 306 VFs were detected in the metagenomic data. We revealed the enrichment of adenomas with VFs involved in cell adhesion, whereas in the early stages of CRC they were enriched in both adhesins and isochorismatase. Advanced stages of CRC were enriched with microbial siderophores, especially enterobactin, which was significantly associated with isochorismate synthase. We highlighted higher abundances of porins and transporters involved in antibiotic resistance and the development of biofilm in advanced stages of CRC. Most VFs detected in CRC, particularly in advanced stages, were shown to be included in siderophore biosynthesis pathways. This enrichment of predicted VFs supports the key role of the gut microbiota in the disease.https://www.mdpi.com/2076-2607/10/12/2365colorectal cancergut microbiotapathogensvirulence factorssiderophoresprognosis |
| spellingShingle | Nour El Houda Mathlouthi Aicha Kriaa Leila Ammar Keskes Moez Rhimi Radhouane Gdoura Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages Microorganisms colorectal cancer gut microbiota pathogens virulence factors siderophores prognosis |
| title | Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages |
| title_full | Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages |
| title_fullStr | Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages |
| title_full_unstemmed | Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages |
| title_short | Virulence Factors in Colorectal Cancer Metagenomes and Association of Microbial Siderophores with Advanced Stages |
| title_sort | virulence factors in colorectal cancer metagenomes and association of microbial siderophores with advanced stages |
| topic | colorectal cancer gut microbiota pathogens virulence factors siderophores prognosis |
| url | https://www.mdpi.com/2076-2607/10/12/2365 |
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