In vivo function of Pgβglu-1 in the release of acetophenones in white spruce
Eastern spruce budworm (Choristoneura fumiferiana Clemens) (ESBW) is a major forest pest which feeds on young shoots of white spruce (Picea glauca) and can cause landscape level economic and ecological losses. Release of acetophenone metabolites, piceol and pungenol, from their corresponding glycosi...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2017-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/3535.pdf |
| _version_ | 1797419966305140736 |
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| author | Melissa H. Mageroy Denis Lachance Sharon Jancsik Geneviève Parent Armand Séguin John Mackay Joerg Bohlmann |
| author_facet | Melissa H. Mageroy Denis Lachance Sharon Jancsik Geneviève Parent Armand Séguin John Mackay Joerg Bohlmann |
| author_sort | Melissa H. Mageroy |
| collection | DOAJ |
| description | Eastern spruce budworm (Choristoneura fumiferiana Clemens) (ESBW) is a major forest pest which feeds on young shoots of white spruce (Picea glauca) and can cause landscape level economic and ecological losses. Release of acetophenone metabolites, piceol and pungenol, from their corresponding glycosides, picein and pungenin, can confer natural resistance of spruce to ESBW. A beta-glucosidase gene, Pgβglu-1, was recently discovered and the encoded enzyme was characterized in vitro to function in the release of the defensive acetophenone aglycons. Here we describe overexpression of Pgβglu-1 in a white spruce genotype whose metabolome contains the glucosylated acetophenones, but no detectable amounts of the aglycons. Transgenic overexpression of Pgβglu-1 resulted in release of the acetophenone aglycons in planta. This work provides in vivo evidence for the function of Pgβglu-1. |
| first_indexed | 2024-03-09T06:54:51Z |
| format | Article |
| id | doaj.art-1bbf6aad72d2437b978f4a4ab5e24f34 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:54:51Z |
| publishDate | 2017-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-1bbf6aad72d2437b978f4a4ab5e24f342023-12-03T10:04:55ZengPeerJ Inc.PeerJ2167-83592017-07-015e353510.7717/peerj.3535In vivo function of Pgβglu-1 in the release of acetophenones in white spruceMelissa H. Mageroy0Denis Lachance1Sharon Jancsik2Geneviève Parent3Armand Séguin4John Mackay5Joerg Bohlmann6Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaLaurentian Forestry Centre, Natural Resources Canada, Quebec, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaDepartment of Wood and Forest Sciences, Laval University, Quebec, CanadaLaurentian Forestry Centre, Natural Resources Canada, Quebec, CanadaDepartment of Wood and Forest Sciences, Laval University, Quebec, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaEastern spruce budworm (Choristoneura fumiferiana Clemens) (ESBW) is a major forest pest which feeds on young shoots of white spruce (Picea glauca) and can cause landscape level economic and ecological losses. Release of acetophenone metabolites, piceol and pungenol, from their corresponding glycosides, picein and pungenin, can confer natural resistance of spruce to ESBW. A beta-glucosidase gene, Pgβglu-1, was recently discovered and the encoded enzyme was characterized in vitro to function in the release of the defensive acetophenone aglycons. Here we describe overexpression of Pgβglu-1 in a white spruce genotype whose metabolome contains the glucosylated acetophenones, but no detectable amounts of the aglycons. Transgenic overexpression of Pgβglu-1 resulted in release of the acetophenone aglycons in planta. This work provides in vivo evidence for the function of Pgβglu-1.https://peerj.com/articles/3535.pdfPicea glaucaConifer defenseSpruce budwormBeta-glucosidaseTransgenic spruce |
| spellingShingle | Melissa H. Mageroy Denis Lachance Sharon Jancsik Geneviève Parent Armand Séguin John Mackay Joerg Bohlmann In vivo function of Pgβglu-1 in the release of acetophenones in white spruce PeerJ Picea glauca Conifer defense Spruce budworm Beta-glucosidase Transgenic spruce |
| title | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce |
| title_full | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce |
| title_fullStr | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce |
| title_full_unstemmed | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce |
| title_short | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce |
| title_sort | in vivo function of pgβglu 1 in the release of acetophenones in white spruce |
| topic | Picea glauca Conifer defense Spruce budworm Beta-glucosidase Transgenic spruce |
| url | https://peerj.com/articles/3535.pdf |
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