Effect of seminal plasma and egg yolk concentration on freezability of goat semen
The objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial va...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zootecnia
2014-10-01
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| Series: | Revista Brasileira de Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000513&lng=en&tlng=en |
| _version_ | 1818133858602713088 |
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| author | Valéria da Silva Ferreira Marco Roberto Bourg de Mello Carlos Elysio Moreira da Fonseca Állan César Ferreira Dias Jéssica Machado Cardoso Rebecca Barbosa Silva Wagner Pereira Martins Júnior |
| author_facet | Valéria da Silva Ferreira Marco Roberto Bourg de Mello Carlos Elysio Moreira da Fonseca Állan César Ferreira Dias Jéssica Machado Cardoso Rebecca Barbosa Silva Wagner Pereira Martins Júnior |
| author_sort | Valéria da Silva Ferreira |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial vagina method at the end of breeding season (June-July). The extender used was the yolk citrate, which was split into two equal aliquots: 5% egg yolk (2.5 mL egg yolk: 47.5 mL citrate solution) were added to one of the samples and 10% egg yolk (5.0 mL egg yolk: 45.0 mL citrate solution) were added to another. The sperm motility and vigor after thawing and post thermal resistance test (TRT) were evaluated and the data were subjected to analysis of variance and means were compared by the F test at 5.0% probability. The observed values for motility and vigor after thawing and post thermal resistance test (TRT), fast and slow, according to the presence of seminal plasma and egg yolk percentage were: 5% egg yolk with plasma (25.0% and 3.3; 1.60% and 0.7; 12.36% and 1.6, respectively); 5% egg yolk without plasma (23.61% and 3.1; 1.25% and 0.2; 9.93% and 1.3, respectively); 10% egg yolk with plasma (30.8% and 3.3; 4.4% and 1.9; 19.5% and 2.7, respectively); and 10% egg yolk without plasma (13.4% and 2.5; 4.1% and 0.5; 17.0% and 1.0, respectively). There were significant differences between the analyzed data in relation to semen with or without plasma at different percentages of egg yolk, and the group that presented the best results was 10% egg yolk citrate in extender with plasma. The presence of seminal plasma and higher concentration of egg yolk in extender provide a higher viability of cryopreserved goat semen. |
| first_indexed | 2024-12-11T08:59:24Z |
| format | Article |
| id | doaj.art-1bc145908e6e402ba790452d6b2d6363 |
| institution | Directory Open Access Journal |
| issn | 1806-9290 |
| language | English |
| last_indexed | 2024-12-11T08:59:24Z |
| publishDate | 2014-10-01 |
| publisher | Sociedade Brasileira de Zootecnia |
| record_format | Article |
| series | Revista Brasileira de Zootecnia |
| spelling | doaj.art-1bc145908e6e402ba790452d6b2d63632022-12-22T01:13:48ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902014-10-01431051351810.1590/S1516-35982014001000001S1516-35982014001000513Effect of seminal plasma and egg yolk concentration on freezability of goat semenValéria da Silva FerreiraMarco Roberto Bourg de MelloCarlos Elysio Moreira da FonsecaÁllan César Ferreira DiasJéssica Machado CardosoRebecca Barbosa SilvaWagner Pereira Martins JúniorThe objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial vagina method at the end of breeding season (June-July). The extender used was the yolk citrate, which was split into two equal aliquots: 5% egg yolk (2.5 mL egg yolk: 47.5 mL citrate solution) were added to one of the samples and 10% egg yolk (5.0 mL egg yolk: 45.0 mL citrate solution) were added to another. The sperm motility and vigor after thawing and post thermal resistance test (TRT) were evaluated and the data were subjected to analysis of variance and means were compared by the F test at 5.0% probability. The observed values for motility and vigor after thawing and post thermal resistance test (TRT), fast and slow, according to the presence of seminal plasma and egg yolk percentage were: 5% egg yolk with plasma (25.0% and 3.3; 1.60% and 0.7; 12.36% and 1.6, respectively); 5% egg yolk without plasma (23.61% and 3.1; 1.25% and 0.2; 9.93% and 1.3, respectively); 10% egg yolk with plasma (30.8% and 3.3; 4.4% and 1.9; 19.5% and 2.7, respectively); and 10% egg yolk without plasma (13.4% and 2.5; 4.1% and 0.5; 17.0% and 1.0, respectively). There were significant differences between the analyzed data in relation to semen with or without plasma at different percentages of egg yolk, and the group that presented the best results was 10% egg yolk citrate in extender with plasma. The presence of seminal plasma and higher concentration of egg yolk in extender provide a higher viability of cryopreserved goat semen.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000513&lng=en&tlng=enbuckcentrifugationextender |
| spellingShingle | Valéria da Silva Ferreira Marco Roberto Bourg de Mello Carlos Elysio Moreira da Fonseca Állan César Ferreira Dias Jéssica Machado Cardoso Rebecca Barbosa Silva Wagner Pereira Martins Júnior Effect of seminal plasma and egg yolk concentration on freezability of goat semen Revista Brasileira de Zootecnia buck centrifugation extender |
| title | Effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| title_full | Effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| title_fullStr | Effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| title_full_unstemmed | Effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| title_short | Effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| title_sort | effect of seminal plasma and egg yolk concentration on freezability of goat semen |
| topic | buck centrifugation extender |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000513&lng=en&tlng=en |
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