Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020
Background: Human enteric pathogens in the family Caliciviridae including norovirus (NoV) and sapovirus (SaV) are associated with acute diarrheal disease globally and are considered as one of the viruses with high genetic diversity. Methods: In order to investigate the epidemiology of NoV and SaV in...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S187603412200209X |
| _version_ | 1817981592288624640 |
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| author | Phitchakorn Phengma Pattara Khamrin Nutthawadee Jampanil Arpaporn Yodmeeklin Nuthapong Ukarapol Niwat Maneekarn Kattareeya Kumthip |
| author_facet | Phitchakorn Phengma Pattara Khamrin Nutthawadee Jampanil Arpaporn Yodmeeklin Nuthapong Ukarapol Niwat Maneekarn Kattareeya Kumthip |
| author_sort | Phitchakorn Phengma |
| collection | DOAJ |
| description | Background: Human enteric pathogens in the family Caliciviridae including norovirus (NoV) and sapovirus (SaV) are associated with acute diarrheal disease globally and are considered as one of the viruses with high genetic diversity. Methods: In order to investigate the epidemiology of NoV and SaV in pediatric patients with acute diarrhea in Chiang Mai, Thailand from January 2019 to December 2020, a total of 675 stool specimens were collected and examined for the presence of NoV and SaV by RT-multiplex PCR. Results: 126 (18.7 %) and 6 (0.9 %) stool samples were positive for NoV and SaV, respectively. Mixed infection of NoV and SaV was detected in one patient (0.2 %). Among 10 different NoV strains detected in this study, NoV genogroup II genotype 4 (GII.4) Sydney 2012 was the most predominant genotype (51.2 %) followed by GII.3, GII.2, GII.6, GII.12, GII.7, GII.17, GI.4, GII.14, and GI.3. Interestingly, monthly distribution of NoV genotypes revealed that NoV GII.3 increased dramatically in August 2019, suggesting an outbreak of NoV GII.3 might occur in the community. In addition, 3 genotypes of SaV were detected in this study with SaV GI.1 being the most common genotype (71.4 %) followed by GI.2 and GII.5 (each at 14.3 %). Conclusions: This study demonstrates the prevalence and genetic diversity of NoV and SaV circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand during 2019–2020 and shows an emergence of NoV GII.3 infection in 2019. |
| first_indexed | 2024-04-13T23:08:05Z |
| format | Article |
| id | doaj.art-1bc19253831145b885afaf6df933bed3 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-13T23:08:05Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-1bc19253831145b885afaf6df933bed32022-12-22T02:25:38ZengElsevierJournal of Infection and Public Health1876-03412022-09-0115910131019Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020Phitchakorn Phengma0Pattara Khamrin1Nutthawadee Jampanil2Arpaporn Yodmeeklin3Nuthapong Ukarapol4Niwat Maneekarn5Kattareeya Kumthip6Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, Thailand; Correspondence to: Department of Microbiology, Faculty of Medicine, Chiang Mai University, 110 Intawaroros Rd. Sripoom, Mueang Chiang Mai, Chiang Mai 50200, Thailand.Background: Human enteric pathogens in the family Caliciviridae including norovirus (NoV) and sapovirus (SaV) are associated with acute diarrheal disease globally and are considered as one of the viruses with high genetic diversity. Methods: In order to investigate the epidemiology of NoV and SaV in pediatric patients with acute diarrhea in Chiang Mai, Thailand from January 2019 to December 2020, a total of 675 stool specimens were collected and examined for the presence of NoV and SaV by RT-multiplex PCR. Results: 126 (18.7 %) and 6 (0.9 %) stool samples were positive for NoV and SaV, respectively. Mixed infection of NoV and SaV was detected in one patient (0.2 %). Among 10 different NoV strains detected in this study, NoV genogroup II genotype 4 (GII.4) Sydney 2012 was the most predominant genotype (51.2 %) followed by GII.3, GII.2, GII.6, GII.12, GII.7, GII.17, GI.4, GII.14, and GI.3. Interestingly, monthly distribution of NoV genotypes revealed that NoV GII.3 increased dramatically in August 2019, suggesting an outbreak of NoV GII.3 might occur in the community. In addition, 3 genotypes of SaV were detected in this study with SaV GI.1 being the most common genotype (71.4 %) followed by GI.2 and GII.5 (each at 14.3 %). Conclusions: This study demonstrates the prevalence and genetic diversity of NoV and SaV circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand during 2019–2020 and shows an emergence of NoV GII.3 infection in 2019.http://www.sciencedirect.com/science/article/pii/S187603412200209XDiarrheaGastroenteritisGenotypeNorovirusSapovirusThailand |
| spellingShingle | Phitchakorn Phengma Pattara Khamrin Nutthawadee Jampanil Arpaporn Yodmeeklin Nuthapong Ukarapol Niwat Maneekarn Kattareeya Kumthip Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 Journal of Infection and Public Health Diarrhea Gastroenteritis Genotype Norovirus Sapovirus Thailand |
| title | Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 |
| title_full | Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 |
| title_fullStr | Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 |
| title_full_unstemmed | Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 |
| title_short | Molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in Thailand, 2019–2020 |
| title_sort | molecular epidemiology and characterization of norovirus and sapovirus in pediatric patients with acute diarrhea in thailand 2019 2020 |
| topic | Diarrhea Gastroenteritis Genotype Norovirus Sapovirus Thailand |
| url | http://www.sciencedirect.com/science/article/pii/S187603412200209X |
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