Recent Advances in Directed Yeast Genome Evolution
<i>Saccharomyces cerevisiae</i>, as a Generally Recognized as Safe (GRAS) fungus, has become one of the most widely used chassis cells for industrial applications and basic research. However, owing to its complex genetic background and intertwined metabolic networks, there are still many...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/6/635 |
| _version_ | 1797485602437857280 |
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| author | Zhen Yao Qinhong Wang Zongjie Dai |
| author_facet | Zhen Yao Qinhong Wang Zongjie Dai |
| author_sort | Zhen Yao |
| collection | DOAJ |
| description | <i>Saccharomyces cerevisiae</i>, as a Generally Recognized as Safe (GRAS) fungus, has become one of the most widely used chassis cells for industrial applications and basic research. However, owing to its complex genetic background and intertwined metabolic networks, there are still many obstacles that need to be overcome in order to improve desired traits and to successfully link genotypes to phenotypes. In this context, genome editing and evolutionary technology have rapidly progressed over the last few decades to facilitate the rapid generation of tailor-made properties as well as for the precise determination of relevant gene targets that regulate physiological functions, including stress resistance, metabolic-pathway optimization and organismal adaptation. Directed genome evolution has emerged as a versatile tool to enable researchers to access desired traits and to study increasingly complicated phenomena. Here, the development of directed genome evolutions in <i>S. cerevisiae</i> is reviewed, with a focus on different techniques driving evolutionary engineering. |
| first_indexed | 2024-03-09T23:21:10Z |
| format | Article |
| id | doaj.art-1bc1a0ba0ef24452848767915426bbd9 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:21:10Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-1bc1a0ba0ef24452848767915426bbd92023-11-23T17:25:22ZengMDPI AGJournal of Fungi2309-608X2022-06-018663510.3390/jof8060635Recent Advances in Directed Yeast Genome EvolutionZhen Yao0Qinhong Wang1Zongjie Dai2Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China<i>Saccharomyces cerevisiae</i>, as a Generally Recognized as Safe (GRAS) fungus, has become one of the most widely used chassis cells for industrial applications and basic research. However, owing to its complex genetic background and intertwined metabolic networks, there are still many obstacles that need to be overcome in order to improve desired traits and to successfully link genotypes to phenotypes. In this context, genome editing and evolutionary technology have rapidly progressed over the last few decades to facilitate the rapid generation of tailor-made properties as well as for the precise determination of relevant gene targets that regulate physiological functions, including stress resistance, metabolic-pathway optimization and organismal adaptation. Directed genome evolution has emerged as a versatile tool to enable researchers to access desired traits and to study increasingly complicated phenomena. Here, the development of directed genome evolutions in <i>S. cerevisiae</i> is reviewed, with a focus on different techniques driving evolutionary engineering.https://www.mdpi.com/2309-608X/8/6/635<i>Saccharomyces cerevisiae</i>evolutionary engineeringsynthetic biologyCRISPR/Cas9SCRaMbLE |
| spellingShingle | Zhen Yao Qinhong Wang Zongjie Dai Recent Advances in Directed Yeast Genome Evolution Journal of Fungi <i>Saccharomyces cerevisiae</i> evolutionary engineering synthetic biology CRISPR/Cas9 SCRaMbLE |
| title | Recent Advances in Directed Yeast Genome Evolution |
| title_full | Recent Advances in Directed Yeast Genome Evolution |
| title_fullStr | Recent Advances in Directed Yeast Genome Evolution |
| title_full_unstemmed | Recent Advances in Directed Yeast Genome Evolution |
| title_short | Recent Advances in Directed Yeast Genome Evolution |
| title_sort | recent advances in directed yeast genome evolution |
| topic | <i>Saccharomyces cerevisiae</i> evolutionary engineering synthetic biology CRISPR/Cas9 SCRaMbLE |
| url | https://www.mdpi.com/2309-608X/8/6/635 |
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