Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure
Objective: Quercetin-3-O-β-D-glucuronide (QG) can alleviate immunological bone marrow failure (BMF) by increasing platelet counts. However, the principal mechanism is less known. This study aimed at deciphering the possible underlying mechanism of QG that is indicated in thrombocytopenic purpura. Me...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
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| Online Access: | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=115;epage=122;aulast=Xia |
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| author | Le-Min Xia Ai-Ping Zhang Qin Zheng Jie Ding Zhe Jin Hai Yu Wan-Hui Wong He-Ping Yu |
| author_facet | Le-Min Xia Ai-Ping Zhang Qin Zheng Jie Ding Zhe Jin Hai Yu Wan-Hui Wong He-Ping Yu |
| author_sort | Le-Min Xia |
| collection | DOAJ |
| description | Objective: Quercetin-3-O-β-D-glucuronide (QG) can alleviate immunological bone marrow failure (BMF) by increasing platelet counts. However, the principal mechanism is less known. This study aimed at deciphering the possible underlying mechanism of QG that is indicated in thrombocytopenic purpura. Methods: In vitro and in vivo experiments were carried out for investigating the mechanism behind QG-facilitated inhibition of mitochondrial pathway-mediated excessive apoptosis of platelets through the phosphatidylinositol-3-kinase (PI3K)/AKT pathway. Results: Our results revealed that QG, the main effective ingredient of Herba Sarcandrae, increases the number of platelets and decreases the expression of Bax, Bad, Bid, and caspase-9 in immunological BMF, indicating the inhibition of mitochondrial pathway-mediated apoptosis. Moreover, we found that the protein and mRNA expressions, as well as the phosphorylated levels of PI3K and AKT, were increased significantly by QG, suggesting the activation of the PI3K/AKT pathway. Furthermore, the inhibition of the PI3K/AKT pathway by LY294002 antagonizes the effects of QG on platelet counts and mitochondrial pathway-mediated apoptosis. Conclusion: We demonstrate that QG inhibits the mitochondria pathway-mediated platelet apoptosis via the PI3K/AKT pathway in immunological BMF. This study thus sheds light on exploring the possible regulatory mechanism of traditional Chinese medicine in the treatment of thrombocytopenia induced by BMF. |
| first_indexed | 2024-04-11T17:59:25Z |
| format | Article |
| id | doaj.art-1bc2ff2936644694bb1cfc351e9a04bc |
| institution | Directory Open Access Journal |
| issn | 2311-8571 |
| language | English |
| last_indexed | 2024-04-11T17:59:25Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj.art-1bc2ff2936644694bb1cfc351e9a04bc2022-12-22T04:10:34ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712022-01-018111512210.4103/wjtcm.wjtcm_44_21Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failureLe-Min XiaAi-Ping ZhangQin ZhengJie DingZhe JinHai YuWan-Hui WongHe-Ping YuObjective: Quercetin-3-O-β-D-glucuronide (QG) can alleviate immunological bone marrow failure (BMF) by increasing platelet counts. However, the principal mechanism is less known. This study aimed at deciphering the possible underlying mechanism of QG that is indicated in thrombocytopenic purpura. Methods: In vitro and in vivo experiments were carried out for investigating the mechanism behind QG-facilitated inhibition of mitochondrial pathway-mediated excessive apoptosis of platelets through the phosphatidylinositol-3-kinase (PI3K)/AKT pathway. Results: Our results revealed that QG, the main effective ingredient of Herba Sarcandrae, increases the number of platelets and decreases the expression of Bax, Bad, Bid, and caspase-9 in immunological BMF, indicating the inhibition of mitochondrial pathway-mediated apoptosis. Moreover, we found that the protein and mRNA expressions, as well as the phosphorylated levels of PI3K and AKT, were increased significantly by QG, suggesting the activation of the PI3K/AKT pathway. Furthermore, the inhibition of the PI3K/AKT pathway by LY294002 antagonizes the effects of QG on platelet counts and mitochondrial pathway-mediated apoptosis. Conclusion: We demonstrate that QG inhibits the mitochondria pathway-mediated platelet apoptosis via the PI3K/AKT pathway in immunological BMF. This study thus sheds light on exploring the possible regulatory mechanism of traditional Chinese medicine in the treatment of thrombocytopenia induced by BMF.http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=115;epage=122;aulast=Xiaapoptosis; mitochondrial pathway; phosphatidylinositol-3-kinase/akt; platelets; quercetin-3-o-β-d-glucuronide |
| spellingShingle | Le-Min Xia Ai-Ping Zhang Qin Zheng Jie Ding Zhe Jin Hai Yu Wan-Hui Wong He-Ping Yu Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure World Journal of Traditional Chinese Medicine apoptosis; mitochondrial pathway; phosphatidylinositol-3-kinase/akt; platelets; quercetin-3-o-β-d-glucuronide |
| title | Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure |
| title_full | Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure |
| title_fullStr | Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure |
| title_full_unstemmed | Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure |
| title_short | Quercetin-3-O-β-D-glucuronide inhibits mitochondria pathway-mediated platelet apoptosis via the phosphatidylinositol-3-kinase/AKT pathway in immunological bone marrow failure |
| title_sort | quercetin 3 o β d glucuronide inhibits mitochondria pathway mediated platelet apoptosis via the phosphatidylinositol 3 kinase akt pathway in immunological bone marrow failure |
| topic | apoptosis; mitochondrial pathway; phosphatidylinositol-3-kinase/akt; platelets; quercetin-3-o-β-d-glucuronide |
| url | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=115;epage=122;aulast=Xia |
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