Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues
Many oral diseases, such as oral leukoplakia and erythroplakia, which have a high potential for malignant transformations, cause abnormal structural changes in the oral mucosa. These changes are clinically assessed by visual inspection and palpation despite their poor accuracy and subjective nature....
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| Format: | Article |
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MDPI AG
2018-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/10/3378 |
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| author | Shekh Emran Reijo Lappalainen Arja M. Kullaa Sami Myllymaa |
| author_facet | Shekh Emran Reijo Lappalainen Arja M. Kullaa Sami Myllymaa |
| author_sort | Shekh Emran |
| collection | DOAJ |
| description | Many oral diseases, such as oral leukoplakia and erythroplakia, which have a high potential for malignant transformations, cause abnormal structural changes in the oral mucosa. These changes are clinically assessed by visual inspection and palpation despite their poor accuracy and subjective nature. We hypothesized that non-invasive bioimpedance spectroscopy (BIS) might be a viable option to improve the diagnostics of potentially malignant lesions. In this study, we aimed to design and optimize the measurement setup and to conduct feasibility testing on pork oral tissues. The contact pressure between a custom-made concentric ring probe and tissue was experimentally optimized. The effects of loading time and inter-electrode spacing on BIS spectra were also clarified. Tissue differentiation testing was performed for ex vivo pork oral tissues including palatinum, buccal mucosa, fat, and muscle tissue samples. We observed that the most reproducible results were obtained by using a loading weight of 200 g and a fixed time period under press, which was necessary to allow meaningful quantitative comparison. All studied tissues showed their own unique spectra, accompanied by significant differences in both impedance magnitude and phase (p ≤ 0.014, Kruskal-Wallis test). BIS shows promise, and further studies are warranted to clarify its potential to detect specific pathological tissue alterations. |
| first_indexed | 2024-04-13T07:50:56Z |
| format | Article |
| id | doaj.art-1bca8ee477a148bfa0834ed5c98fba6f |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T07:50:56Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1bca8ee477a148bfa0834ed5c98fba6f2022-12-22T02:55:32ZengMDPI AGSensors1424-82202018-10-011810337810.3390/s18103378s18103378Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral TissuesShekh Emran0Reijo Lappalainen1Arja M. Kullaa2Sami Myllymaa3SIB Labs, Department of Applied Physics, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, FinlandSIB Labs, Department of Applied Physics, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, FinlandInstitute of Dentistry, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, FinlandSIB Labs, Department of Applied Physics, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, FinlandMany oral diseases, such as oral leukoplakia and erythroplakia, which have a high potential for malignant transformations, cause abnormal structural changes in the oral mucosa. These changes are clinically assessed by visual inspection and palpation despite their poor accuracy and subjective nature. We hypothesized that non-invasive bioimpedance spectroscopy (BIS) might be a viable option to improve the diagnostics of potentially malignant lesions. In this study, we aimed to design and optimize the measurement setup and to conduct feasibility testing on pork oral tissues. The contact pressure between a custom-made concentric ring probe and tissue was experimentally optimized. The effects of loading time and inter-electrode spacing on BIS spectra were also clarified. Tissue differentiation testing was performed for ex vivo pork oral tissues including palatinum, buccal mucosa, fat, and muscle tissue samples. We observed that the most reproducible results were obtained by using a loading weight of 200 g and a fixed time period under press, which was necessary to allow meaningful quantitative comparison. All studied tissues showed their own unique spectra, accompanied by significant differences in both impedance magnitude and phase (p ≤ 0.014, Kruskal-Wallis test). BIS shows promise, and further studies are warranted to clarify its potential to detect specific pathological tissue alterations.http://www.mdpi.com/1424-8220/18/10/3378bioimpedance spectroscopyelectrical impedance spectroscopyelectrical characterizationprobesoft tissueoral cancerpotentially malignant disordersoral mucosa |
| spellingShingle | Shekh Emran Reijo Lappalainen Arja M. Kullaa Sami Myllymaa Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues Sensors bioimpedance spectroscopy electrical impedance spectroscopy electrical characterization probe soft tissue oral cancer potentially malignant disorders oral mucosa |
| title | Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues |
| title_full | Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues |
| title_fullStr | Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues |
| title_full_unstemmed | Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues |
| title_short | Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues |
| title_sort | concentric ring probe for bioimpedance spectroscopic measurements design and ex vivo feasibility testing on pork oral tissues |
| topic | bioimpedance spectroscopy electrical impedance spectroscopy electrical characterization probe soft tissue oral cancer potentially malignant disorders oral mucosa |
| url | http://www.mdpi.com/1424-8220/18/10/3378 |
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