Identification of Leaf Promoters for Use in Transgenic Wheat
Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2223-7747/7/2/27 |
| _version_ | 1819005090014429184 |
|---|---|
| author | Saqer S. Alotaibi Caroline A. Sparks Martin A. J. Parry Andrew J. Simkin Christine A. Raines |
| author_facet | Saqer S. Alotaibi Caroline A. Sparks Martin A. J. Parry Andrew J. Simkin Christine A. Raines |
| author_sort | Saqer S. Alotaibi |
| collection | DOAJ |
| description | Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields. |
| first_indexed | 2024-12-20T23:47:15Z |
| format | Article |
| id | doaj.art-1bcfd6c5072a4a1ca50548d9da2a42cf |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-20T23:47:15Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1bcfd6c5072a4a1ca50548d9da2a42cf2022-12-21T19:22:55ZengMDPI AGPlants2223-77472018-03-01722710.3390/plants7020027plants7020027Identification of Leaf Promoters for Use in Transgenic WheatSaqer S. Alotaibi0Caroline A. Sparks1Martin A. J. Parry2Andrew J. Simkin3Christine A. Raines4School of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UKRothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UKRothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UKSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UKSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UKWheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.http://www.mdpi.com/2223-7747/7/2/27promoterwheattissue specificphotosynthesisreporter geneyield |
| spellingShingle | Saqer S. Alotaibi Caroline A. Sparks Martin A. J. Parry Andrew J. Simkin Christine A. Raines Identification of Leaf Promoters for Use in Transgenic Wheat Plants promoter wheat tissue specific photosynthesis reporter gene yield |
| title | Identification of Leaf Promoters for Use in Transgenic Wheat |
| title_full | Identification of Leaf Promoters for Use in Transgenic Wheat |
| title_fullStr | Identification of Leaf Promoters for Use in Transgenic Wheat |
| title_full_unstemmed | Identification of Leaf Promoters for Use in Transgenic Wheat |
| title_short | Identification of Leaf Promoters for Use in Transgenic Wheat |
| title_sort | identification of leaf promoters for use in transgenic wheat |
| topic | promoter wheat tissue specific photosynthesis reporter gene yield |
| url | http://www.mdpi.com/2223-7747/7/2/27 |
| work_keys_str_mv | AT saqersalotaibi identificationofleafpromotersforuseintransgenicwheat AT carolineasparks identificationofleafpromotersforuseintransgenicwheat AT martinajparry identificationofleafpromotersforuseintransgenicwheat AT andrewjsimkin identificationofleafpromotersforuseintransgenicwheat AT christinearaines identificationofleafpromotersforuseintransgenicwheat |