Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species
Background: Colorectal cancer, a major health concern worldwide, is the third mostcommon form of cancer and second leading cause of cancer-related deaths. Theflavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods andimportant components of the human diet. Luteolin, a bioflav...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2013-10-01
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| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | http://mejc.sums.ac.ir/index.php/mejc/article/view/118/107 |
| _version_ | 1818676870547243008 |
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| author | Ashok Kumar Pandurangan Sudhandiran Ganapasam |
| author_facet | Ashok Kumar Pandurangan Sudhandiran Ganapasam |
| author_sort | Ashok Kumar Pandurangan |
| collection | DOAJ |
| description | Background: Colorectal cancer, a major health concern worldwide, is the third mostcommon form of cancer and second leading cause of cancer-related deaths. Theflavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods andimportant components of the human diet. Luteolin, a bioflavonoid, possesses manybeneficial effects including antioxidant, anti-inflammatory, anti-allergic activities. Methods:We used the HCT-15 colon adenocarcinoma cell line in this study. Cellswere treated with luteolin (100 µM). Results: Membrane damage markers such as alkaline phosphatase and lactatedehydrogenase were analyzed in a time-dependent manner. Luteolin increased reactiveoxygen species in a time-dependent manner. DNA damage, a hallmark of apoptosis,was induced by luteolin as analyzed by agarose gel electrophoresis. Conclusion: Luteolin acts as a potential cytotoxic agent that can be used to treatcolorectal cancer. |
| first_indexed | 2024-12-17T08:50:21Z |
| format | Article |
| id | doaj.art-1bd3628cc0d84dd790e059f5d2aee8ac |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-12-17T08:50:21Z |
| publishDate | 2013-10-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-1bd3628cc0d84dd790e059f5d2aee8ac2022-12-21T21:56:06ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872013-10-0144175180Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen SpeciesAshok Kumar PanduranganSudhandiran GanapasamBackground: Colorectal cancer, a major health concern worldwide, is the third mostcommon form of cancer and second leading cause of cancer-related deaths. Theflavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods andimportant components of the human diet. Luteolin, a bioflavonoid, possesses manybeneficial effects including antioxidant, anti-inflammatory, anti-allergic activities. Methods:We used the HCT-15 colon adenocarcinoma cell line in this study. Cellswere treated with luteolin (100 µM). Results: Membrane damage markers such as alkaline phosphatase and lactatedehydrogenase were analyzed in a time-dependent manner. Luteolin increased reactiveoxygen species in a time-dependent manner. DNA damage, a hallmark of apoptosis,was induced by luteolin as analyzed by agarose gel electrophoresis. Conclusion: Luteolin acts as a potential cytotoxic agent that can be used to treatcolorectal cancer.http://mejc.sums.ac.ir/index.php/mejc/article/view/118/107Colon cancerLuteolinROSHCT-15 |
| spellingShingle | Ashok Kumar Pandurangan Sudhandiran Ganapasam Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species Middle East Journal of Cancer Colon cancer Luteolin ROS HCT-15 |
| title | Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species |
| title_full | Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species |
| title_fullStr | Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species |
| title_full_unstemmed | Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species |
| title_short | Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species |
| title_sort | cytotoxic effect of luteolin on human colorectal cancer cell line hct 15 crucial involvement of reactive oxygen species |
| topic | Colon cancer Luteolin ROS HCT-15 |
| url | http://mejc.sums.ac.ir/index.php/mejc/article/view/118/107 |
| work_keys_str_mv | AT ashokkumarpandurangan cytotoxiceffectofluteolinonhumancolorectalcancercelllinehct15crucialinvolvementofreactiveoxygenspecies AT sudhandiranganapasam cytotoxiceffectofluteolinonhumancolorectalcancercelllinehct15crucialinvolvementofreactiveoxygenspecies |