An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production

An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production

Abstract Background The CRISPR/Cas9 technology is being employed as a convenient tool for genetic engineering of the industrially important filamentous fungus Trichoderma reesei. However, multiplex gene editing is still constrained by the sgRNA processing capability, hindering strain improvement of...

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Bibliographic Details
Main Authors: Jiaxin Zhang, Kehang Li, Yu Sun, Cheng Yao, Weifeng Liu, Hong Liu, Yaohua Zhong
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Biotechnology for Biofuels and Bioproducts
Subjects:
CRISPR/Cas9
Trichoderma reesei
Multigene editing
Glucose oxidase
Lignocellulose-degrading enzymes
Multiple sgRNA processing
Online Access:https://doi.org/10.1186/s13068-024-02468-7

Internet

https://doi.org/10.1186/s13068-024-02468-7

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