EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer
It is known that miRNA-378a-3p (miR-378) could be induced by eicosapentaenoic acid (EPA), an omega-3 fatty acid. Herein, we first demonstrated how miR-378 exerts anti-prostate cancer (PCa) actions by influencing multiple target genes, including KLK2, KLK4, KLK6, and KLK14, which are implicated in PC...
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MDPI AG
2022-06-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/11/2813 |
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| author | Kai-Jie Yu De-Yi Ji Ming-Li Hsieh Cheng-Keng Chuang See-Tong Pang Wen-Hui Weng |
| author_facet | Kai-Jie Yu De-Yi Ji Ming-Li Hsieh Cheng-Keng Chuang See-Tong Pang Wen-Hui Weng |
| author_sort | Kai-Jie Yu |
| collection | DOAJ |
| description | It is known that miRNA-378a-3p (miR-378) could be induced by eicosapentaenoic acid (EPA), an omega-3 fatty acid. Herein, we first demonstrated how miR-378 exerts anti-prostate cancer (PCa) actions by influencing multiple target genes, including KLK2, KLK4, KLK6, and KLK14, which are implicated in PCa development, cell proliferation, and cell survival. Furthermore, these genes also correlate with androgen and mTOR signaling transduction, and are considered pivotal pathways for the onset and progression of PCa. In total, four PCa cell lines and eight pairing tissues (tumor vs. normal) from clinical PCa patients were included in the current study. The results showed high significance after EPA induced tumor cells containing higher expression levels of miR-378, and led the PCa cells having low cell viabilities, and they progressed to apoptosis when compared with normal prostate cells (<i>p</i> < 0.001). The findings indicated that EPA might become a potential therapy for PCa, especially because it is derived from the components of natural fish oil; it may prove to be a great help for solving the problem of castration-resistant prostate cancer (CRPC). |
| first_indexed | 2024-03-10T01:25:32Z |
| format | Article |
| id | doaj.art-1bd4af36cd974559ac8ea4164a17534f |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T01:25:32Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-1bd4af36cd974559ac8ea4164a17534f2023-11-23T13:51:36ZengMDPI AGCancers2072-66942022-06-011411281310.3390/cancers14112813EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate CancerKai-Jie Yu0De-Yi Ji1Ming-Li Hsieh2Cheng-Keng Chuang3See-Tong Pang4Wen-Hui Weng5Department of Chemical Engineering and Biotechnology, Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanDepartment of Chemical Engineering and Biotechnology, Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanDepartment of Urology, Chang Gung Memorial Hospital, Tao-Yuan 333, TaiwanDepartment of Urology, Chang Gung Memorial Hospital, Tao-Yuan 333, TaiwanDepartment of Urology, Chang Gung Memorial Hospital, Tao-Yuan 333, TaiwanDepartment of Chemical Engineering and Biotechnology, Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanIt is known that miRNA-378a-3p (miR-378) could be induced by eicosapentaenoic acid (EPA), an omega-3 fatty acid. Herein, we first demonstrated how miR-378 exerts anti-prostate cancer (PCa) actions by influencing multiple target genes, including KLK2, KLK4, KLK6, and KLK14, which are implicated in PCa development, cell proliferation, and cell survival. Furthermore, these genes also correlate with androgen and mTOR signaling transduction, and are considered pivotal pathways for the onset and progression of PCa. In total, four PCa cell lines and eight pairing tissues (tumor vs. normal) from clinical PCa patients were included in the current study. The results showed high significance after EPA induced tumor cells containing higher expression levels of miR-378, and led the PCa cells having low cell viabilities, and they progressed to apoptosis when compared with normal prostate cells (<i>p</i> < 0.001). The findings indicated that EPA might become a potential therapy for PCa, especially because it is derived from the components of natural fish oil; it may prove to be a great help for solving the problem of castration-resistant prostate cancer (CRPC).https://www.mdpi.com/2072-6694/14/11/2813prostate cancerEPAKLK genesmiR-378 |
| spellingShingle | Kai-Jie Yu De-Yi Ji Ming-Li Hsieh Cheng-Keng Chuang See-Tong Pang Wen-Hui Weng EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer Cancers prostate cancer EPA KLK genes miR-378 |
| title | EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer |
| title_full | EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer |
| title_fullStr | EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer |
| title_full_unstemmed | EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer |
| title_short | EPA Modulates KLK Genes via miR-378: A Potential Therapy in Prostate Cancer |
| title_sort | epa modulates klk genes via mir 378 a potential therapy in prostate cancer |
| topic | prostate cancer EPA KLK genes miR-378 |
| url | https://www.mdpi.com/2072-6694/14/11/2813 |
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