| Summary: | Bioluminescence imaging has been used to visualize the biological effects of human beings and is a promising technique in a recent modality. In this study, the digital image technique is used to improve quality and recover images. The optical fluence that emerges from the source is generated using a camera, and a low resgolution is observed. In this paper, the diurnal change of ultra-weak photon emission was successfully imaged with an improved, highly sensitive imaging system using a charge-coupled device (CCD) camera. The changes in energy metabolism might be linked with diurnal changes in photon emission, and when observed, the body emits extremely weak light spontaneously without external photoexcitation. Therefore, to obtain accurate information, a combined Barn Door Star Tracker approach has been proposed to improve the accuracy of the method and has been implemented to test on celestial bodies. The ability to temporally assess the location of star movement can be monitored accurately with bioluminescence imaging.
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