Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity
Juan Manuel Reséndiz-Hernández,1,2 Raúl H Sansores,3 Rafael de Jesús Hernández-Zenteno,3 Gilberto Vargas-Alarcón,4 Laura Colín-Barenque,5 Mónica Velázquez-Uncal,3 Angel Camarena,1 Alejandra Ram&iac...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2015-06-01
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| Series: | International Journal of COPD |
| Online Access: | http://www.dovepress.com/identification-of-genetic-variants-in-the-tnf-promoter-associated-with-peer-reviewed-article-COPD |
| _version_ | 1811309162096754688 |
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| author | Reséndiz-Hernández JM Sansores RH Hernández-Zenteno RJ Vargas-Alarcón G Colín-Barenque L Velázquez-Uncal M Camarena A Ramírez-Venegas A Falfán-Valencia R |
| author_facet | Reséndiz-Hernández JM Sansores RH Hernández-Zenteno RJ Vargas-Alarcón G Colín-Barenque L Velázquez-Uncal M Camarena A Ramírez-Venegas A Falfán-Valencia R |
| author_sort | Reséndiz-Hernández JM |
| collection | DOAJ |
| description | Juan Manuel Reséndiz-Hernández,1,2 Raúl H Sansores,3 Rafael de Jesús Hernández-Zenteno,3 Gilberto Vargas-Alarcón,4 Laura Colín-Barenque,5 Mónica Velázquez-Uncal,3 Angel Camarena,1 Alejandra Ramírez-Venegas,3 Ramcés Falfán-Valencia1 1Laboratory HLA, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico; 2Graduate Program in Biological Sciences, Universidad Nacional Autónoma de México, Mexico City, Mexico; 3Research Department in smoking and COPD, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico; 4Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico; 5Department of Neuroscience, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla de Baz, Mexico State, Mexico Abstract: Chronic obstructive pulmonary disease (COPD) is an inflammatory disease that arises in response to noxious particles or gases. Associations of genetic polymorphisms in TNF have been reported in Asians and Caucasians, but not in Mestizo populations. A case-control study was conducted in two stages: in the first stage, patients with COPD (COPD group, n=165) and smokers without disease (SNC group, n=165) were included and the TNF promoter sequence was determined using direct sequencing. In the second stage, the identified polymorphisms were validated by real-time polymerase chain reaction (PCR) in COPD (n=260) and SNC (n=506). In the first stage, 11 different sets of “contig” alignments were determined, of which contig 10 was found to be associated with susceptibility (P=5.0E-04, OR [odds ratio] =3.64) and contig 1 with Global Initiative for COPD (GOLD) greater grade (P=1.0E-02, OR =3.82). The single nucleotide polymorphisms found in this region were individually identified; the GA genotypes of rs1800629 (P=0.038, OR =2.07), rs56036015 (P=0.0082, OR =3.18), and rs361525 (P=1.0E-02, OR =4.220) were higher in the COPD group vs the SNC group; after second-stage validation, rs1800629 (P=6.00E-03, OR =2.26) and rs56036015 (P=1.10E-03, OR =2.54) are maintained. There are genetic variants in the TNF promoter associated with increased risk of COPD secondary to smoking and with a higher GOLD grade in the Mexican Mestizo population. Keywords: lung, cigarette smoking, SNP, GOLD, Mexican population |
| first_indexed | 2024-04-13T09:37:25Z |
| format | Article |
| id | doaj.art-1bd73ec835d341b487b667b1a1794285 |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-04-13T09:37:25Z |
| publishDate | 2015-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-1bd73ec835d341b487b667b1a17942852022-12-22T02:52:03ZengDove Medical PressInternational Journal of COPD1178-20052015-06-012015Issue 11241125122367Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severityReséndiz-Hernández JMSansores RHHernández-Zenteno RJVargas-Alarcón GColín-Barenque LVelázquez-Uncal MCamarena ARamírez-Venegas AFalfán-Valencia RJuan Manuel Reséndiz-Hernández,1,2 Raúl H Sansores,3 Rafael de Jesús Hernández-Zenteno,3 Gilberto Vargas-Alarcón,4 Laura Colín-Barenque,5 Mónica Velázquez-Uncal,3 Angel Camarena,1 Alejandra Ramírez-Venegas,3 Ramcés Falfán-Valencia1 1Laboratory HLA, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico; 2Graduate Program in Biological Sciences, Universidad Nacional Autónoma de México, Mexico City, Mexico; 3Research Department in smoking and COPD, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico; 4Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico; 5Department of Neuroscience, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla de Baz, Mexico State, Mexico Abstract: Chronic obstructive pulmonary disease (COPD) is an inflammatory disease that arises in response to noxious particles or gases. Associations of genetic polymorphisms in TNF have been reported in Asians and Caucasians, but not in Mestizo populations. A case-control study was conducted in two stages: in the first stage, patients with COPD (COPD group, n=165) and smokers without disease (SNC group, n=165) were included and the TNF promoter sequence was determined using direct sequencing. In the second stage, the identified polymorphisms were validated by real-time polymerase chain reaction (PCR) in COPD (n=260) and SNC (n=506). In the first stage, 11 different sets of “contig” alignments were determined, of which contig 10 was found to be associated with susceptibility (P=5.0E-04, OR [odds ratio] =3.64) and contig 1 with Global Initiative for COPD (GOLD) greater grade (P=1.0E-02, OR =3.82). The single nucleotide polymorphisms found in this region were individually identified; the GA genotypes of rs1800629 (P=0.038, OR =2.07), rs56036015 (P=0.0082, OR =3.18), and rs361525 (P=1.0E-02, OR =4.220) were higher in the COPD group vs the SNC group; after second-stage validation, rs1800629 (P=6.00E-03, OR =2.26) and rs56036015 (P=1.10E-03, OR =2.54) are maintained. There are genetic variants in the TNF promoter associated with increased risk of COPD secondary to smoking and with a higher GOLD grade in the Mexican Mestizo population. Keywords: lung, cigarette smoking, SNP, GOLD, Mexican populationhttp://www.dovepress.com/identification-of-genetic-variants-in-the-tnf-promoter-associated-with-peer-reviewed-article-COPD |
| spellingShingle | Reséndiz-Hernández JM Sansores RH Hernández-Zenteno RJ Vargas-Alarcón G Colín-Barenque L Velázquez-Uncal M Camarena A Ramírez-Venegas A Falfán-Valencia R Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity International Journal of COPD |
| title | Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity |
| title_full | Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity |
| title_fullStr | Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity |
| title_full_unstemmed | Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity |
| title_short | Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity |
| title_sort | identification of genetic variants in the tnf promoter associated with copd secondary to nbsp tobacco smoking and its severity |
| url | http://www.dovepress.com/identification-of-genetic-variants-in-the-tnf-promoter-associated-with-peer-reviewed-article-COPD |
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