<em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems
Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of these. The aim of this work was to multiply M. royoc using Temporary Immersion Sys...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2011-04-01
|
| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/229 |
| _version_ | 1819064534970662912 |
|---|---|
| author | Elio Jiménez Carlos Reyes Pablo Machado Naivy Pérez-Alonso Alina Capote Anabel Pérez Bettina Eichler-Loebermann |
| author_facet | Elio Jiménez Carlos Reyes Pablo Machado Naivy Pérez-Alonso Alina Capote Anabel Pérez Bettina Eichler-Loebermann |
| author_sort | Elio Jiménez |
| collection | DOAJ |
| description | Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of these. The aim of this work was to multiply M. royoc using Temporary Immersion System (TIS). It was used TIS of 1000 ml capacity. Each contained 250 ml MS medium with 4.4 μM benzyladenine (BA) and 2.9 μM indole acetic acid (IAA). The SIT were inoculated with 30 individual explants (shoot tips and nodal segments). It was determined the effect of the immersion frequency (two, four and six immersions of two minutes per day) and the type of explants (shoot tips, nodal segments) on the multiplication of shoots and biomass production. It was found that with four and six immersions per day the highest values of multiplication coefficient and shoot length were obtained. The maximum biomass production was achieved with six immersions per day. No hyperhidricity symptoms were observed in shoots. It was noted that the nodal segments produced more shoots per explant, increased multiplication coefficient and biomass than the apex, while the latter resulted in longer shoots.
Keywords: micropropagation, shoots multiplication |
| first_indexed | 2024-12-21T15:32:06Z |
| format | Article |
| id | doaj.art-1bd773e9139747f5a36fa00978eb6b23 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-21T15:32:06Z |
| publishDate | 2011-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-1bd773e9139747f5a36fa00978eb6b232022-12-21T18:58:44ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472011-04-01112204<em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion SystemsElio JiménezCarlos ReyesPablo MachadoNaivy Pérez-AlonsoAlina CapoteAnabel PérezBettina Eichler-LoebermannMorinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of these. The aim of this work was to multiply M. royoc using Temporary Immersion System (TIS). It was used TIS of 1000 ml capacity. Each contained 250 ml MS medium with 4.4 μM benzyladenine (BA) and 2.9 μM indole acetic acid (IAA). The SIT were inoculated with 30 individual explants (shoot tips and nodal segments). It was determined the effect of the immersion frequency (two, four and six immersions of two minutes per day) and the type of explants (shoot tips, nodal segments) on the multiplication of shoots and biomass production. It was found that with four and six immersions per day the highest values of multiplication coefficient and shoot length were obtained. The maximum biomass production was achieved with six immersions per day. No hyperhidricity symptoms were observed in shoots. It was noted that the nodal segments produced more shoots per explant, increased multiplication coefficient and biomass than the apex, while the latter resulted in longer shoots. Keywords: micropropagation, shoots multiplicationhttps://revista.ibp.co.cu/index.php/BV/article/view/229 |
| spellingShingle | Elio Jiménez Carlos Reyes Pablo Machado Naivy Pérez-Alonso Alina Capote Anabel Pérez Bettina Eichler-Loebermann <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems Biotecnología Vegetal |
| title | <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems |
| title_full | <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems |
| title_fullStr | <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems |
| title_full_unstemmed | <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems |
| title_short | <em>In vitro</em> multiplication of <em>Morinda royoc</em> L. in Temporary Immersion Systems |
| title_sort | em in vitro em multiplication of em morinda royoc em l in temporary immersion systems |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/229 |
| work_keys_str_mv | AT eliojimenez eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT carlosreyes eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT pablomachado eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT naivyperezalonso eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT alinacapote eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT anabelperez eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems AT bettinaeichlerloebermann eminvitroemmultiplicationofemmorindaroyocemlintemporaryimmersionsystems |