Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction
The increase in antimicrobial resistance is a threat to both human and animal health. The transfer of antibiotic resistance genes (ARG) via plasmids has been studied in detail whereas the contribution of bacteriophage-mediated ARG transmission is relatively little explored. We isolated and character...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/12/10/1060 |
| _version_ | 1827705608443265024 |
|---|---|
| author | Junlin Liu Peng Liu Fenglin Feng Junxuan Zhang Fulin Li Mianzhi Wang Yongxue Sun |
| author_facet | Junlin Liu Peng Liu Fenglin Feng Junxuan Zhang Fulin Li Mianzhi Wang Yongxue Sun |
| author_sort | Junlin Liu |
| collection | DOAJ |
| description | The increase in antimicrobial resistance is a threat to both human and animal health. The transfer of antibiotic resistance genes (ARG) via plasmids has been studied in detail whereas the contribution of bacteriophage-mediated ARG transmission is relatively little explored. We isolated and characterized two T7-like lytic bacteriophages that infected multidrug-resistant <i>Escherichia coli</i> hosts. The morphology and genomic analysis indicated that both phage HZP2 and HZ2R8 were evolutionarily related and their genomes did not encode ARGs. However, ARG-like raw reads were detected in offspring sequencing data with a different abundance level implying that potential ARG packaging had occurred. PCR results demonstrated that six fragments of genes (<i>qnrS</i>, <i>cmlA</i>, <i>tetM</i>, <i>bla<sub>TEM</sub></i>, <i>sul3</i>, <i>mcr-1</i>) were potentially packaged by phage HZP2 and four (<i>qnrS</i>, <i>cmlA</i>, <i>bla<sub>TEM</sub></i>, <i>mcr-1</i>) by phage HZ2R8. Further quantitative results showed that ARG abundance hierarchies were similar. The gene <i>bla<sub>TEM</sub></i> was the most abundant (up to 1.38 × 10<sup>7</sup> copies/mL) whereas <i>cmlA</i> and <i>qnrS</i> were the least. Moreover, the clinically important <i>mcr-1</i> gene was the second most abundant ARG indicating a possibility for spread through generalized transduction. Together, our results indicated that these structurally similar phage possessed similar characteristics and potential packaging during phage-host interaction displayed an ARG preference rather than occurring randomly. |
| first_indexed | 2024-03-10T16:07:19Z |
| format | Article |
| id | doaj.art-1bd935639d0c4b8ca2c5279b8924cad7 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T16:07:19Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1bd935639d0c4b8ca2c5279b8924cad72023-11-20T14:45:15ZengMDPI AGViruses1999-49152020-09-011210106010.3390/v12101060Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host InteractionJunlin Liu0Peng Liu1Fenglin Feng2Junxuan Zhang3Fulin Li4Mianzhi Wang5Yongxue Sun6National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510640, ChinaThe increase in antimicrobial resistance is a threat to both human and animal health. The transfer of antibiotic resistance genes (ARG) via plasmids has been studied in detail whereas the contribution of bacteriophage-mediated ARG transmission is relatively little explored. We isolated and characterized two T7-like lytic bacteriophages that infected multidrug-resistant <i>Escherichia coli</i> hosts. The morphology and genomic analysis indicated that both phage HZP2 and HZ2R8 were evolutionarily related and their genomes did not encode ARGs. However, ARG-like raw reads were detected in offspring sequencing data with a different abundance level implying that potential ARG packaging had occurred. PCR results demonstrated that six fragments of genes (<i>qnrS</i>, <i>cmlA</i>, <i>tetM</i>, <i>bla<sub>TEM</sub></i>, <i>sul3</i>, <i>mcr-1</i>) were potentially packaged by phage HZP2 and four (<i>qnrS</i>, <i>cmlA</i>, <i>bla<sub>TEM</sub></i>, <i>mcr-1</i>) by phage HZ2R8. Further quantitative results showed that ARG abundance hierarchies were similar. The gene <i>bla<sub>TEM</sub></i> was the most abundant (up to 1.38 × 10<sup>7</sup> copies/mL) whereas <i>cmlA</i> and <i>qnrS</i> were the least. Moreover, the clinically important <i>mcr-1</i> gene was the second most abundant ARG indicating a possibility for spread through generalized transduction. Together, our results indicated that these structurally similar phage possessed similar characteristics and potential packaging during phage-host interaction displayed an ARG preference rather than occurring randomly.https://www.mdpi.com/1999-4915/12/10/1060T7-like phageantibiotic resistance genes (ARG)potential packagingphage-host interaction |
| spellingShingle | Junlin Liu Peng Liu Fenglin Feng Junxuan Zhang Fulin Li Mianzhi Wang Yongxue Sun Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction Viruses T7-like phage antibiotic resistance genes (ARG) potential packaging phage-host interaction |
| title | Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction |
| title_full | Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction |
| title_fullStr | Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction |
| title_full_unstemmed | Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction |
| title_short | Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction |
| title_sort | evaluation of potential arg packaging by two environmental t7 like phage during phage host interaction |
| topic | T7-like phage antibiotic resistance genes (ARG) potential packaging phage-host interaction |
| url | https://www.mdpi.com/1999-4915/12/10/1060 |
| work_keys_str_mv | AT junlinliu evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT pengliu evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT fenglinfeng evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT junxuanzhang evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT fulinli evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT mianzhiwang evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction AT yongxuesun evaluationofpotentialargpackagingbytwoenvironmentalt7likephageduringphagehostinteraction |