Tetraploidy-associated centrosome overduplication in mouse early embryos
Recently, we observed that tetraploidization of certain types of human cancer cells resulted in upregulation of centrosome duplication cycles and chronic generation of the extra centrosome. Here, we investigated whether tetraploidy-linked upregulation of centrosome duplication also occurs in non-can...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-07-01
|
| Series: | Communicative & Integrative Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19420889.2018.1526605 |
| _version_ | 1818929404536946688 |
|---|---|
| author | Kan Yaguchi Takahiro Yamamoto Ryo Matsui Masaya Shimada Atsuko Shibanuma Keiko Kamimura Toshiaki Koda Ryota Uehara |
| author_facet | Kan Yaguchi Takahiro Yamamoto Ryo Matsui Masaya Shimada Atsuko Shibanuma Keiko Kamimura Toshiaki Koda Ryota Uehara |
| author_sort | Kan Yaguchi |
| collection | DOAJ |
| description | Recently, we observed that tetraploidization of certain types of human cancer cells resulted in upregulation of centrosome duplication cycles and chronic generation of the extra centrosome. Here, we investigated whether tetraploidy-linked upregulation of centrosome duplication also occurs in non-cancer cells using tetraploidized parthenogenetic mouse embryos. Cytokinesis blockage at early embryonic stage before de novo centriole biogenesis provided the unique opportunity in which tetraploidization can be induced without transient doubling of centrosome number. The extra numbers of the centrioles and the centrosomes were observed more frequently in tetraploidized embryos during the blastocyst stage than in their diploid counterparts, demonstrating the generality of the newly found tetraploidy-driven centrosome overduplication in mammalian non-cancer systems. |
| first_indexed | 2024-12-20T03:44:16Z |
| format | Article |
| id | doaj.art-1bd9b0ce981e4076999874a85dcb19ff |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-20T03:44:16Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-1bd9b0ce981e4076999874a85dcb19ff2022-12-21T19:54:40ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892018-07-0111410.1080/19420889.2018.15266051526605Tetraploidy-associated centrosome overduplication in mouse early embryosKan Yaguchi0Takahiro Yamamoto1Ryo Matsui2Masaya Shimada3Atsuko Shibanuma4Keiko Kamimura5Toshiaki Koda6Ryota Uehara7Hokkaido UniversityHokkaido UniversityHokkaido UniversityHokkaido UniversityHokkaido UniversityHokkaido UniversityHokkaido UniversityHokkaido UniversityRecently, we observed that tetraploidization of certain types of human cancer cells resulted in upregulation of centrosome duplication cycles and chronic generation of the extra centrosome. Here, we investigated whether tetraploidy-linked upregulation of centrosome duplication also occurs in non-cancer cells using tetraploidized parthenogenetic mouse embryos. Cytokinesis blockage at early embryonic stage before de novo centriole biogenesis provided the unique opportunity in which tetraploidization can be induced without transient doubling of centrosome number. The extra numbers of the centrioles and the centrosomes were observed more frequently in tetraploidized embryos during the blastocyst stage than in their diploid counterparts, demonstrating the generality of the newly found tetraploidy-driven centrosome overduplication in mammalian non-cancer systems.http://dx.doi.org/10.1080/19420889.2018.1526605Tetraploidcentrosomemouse embryo |
| spellingShingle | Kan Yaguchi Takahiro Yamamoto Ryo Matsui Masaya Shimada Atsuko Shibanuma Keiko Kamimura Toshiaki Koda Ryota Uehara Tetraploidy-associated centrosome overduplication in mouse early embryos Communicative & Integrative Biology Tetraploid centrosome mouse embryo |
| title | Tetraploidy-associated centrosome overduplication in mouse early embryos |
| title_full | Tetraploidy-associated centrosome overduplication in mouse early embryos |
| title_fullStr | Tetraploidy-associated centrosome overduplication in mouse early embryos |
| title_full_unstemmed | Tetraploidy-associated centrosome overduplication in mouse early embryos |
| title_short | Tetraploidy-associated centrosome overduplication in mouse early embryos |
| title_sort | tetraploidy associated centrosome overduplication in mouse early embryos |
| topic | Tetraploid centrosome mouse embryo |
| url | http://dx.doi.org/10.1080/19420889.2018.1526605 |
| work_keys_str_mv | AT kanyaguchi tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT takahiroyamamoto tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT ryomatsui tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT masayashimada tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT atsukoshibanuma tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT keikokamimura tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT toshiakikoda tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos AT ryotauehara tetraploidyassociatedcentrosomeoverduplicationinmouseearlyembryos |