Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae
Summary: The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006116 |
| _version_ | 1811292518867795968 |
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| author | Laura Corradi Margherita Zaupa Suphansa Sawamiphak Alessandro Filosa |
| author_facet | Laura Corradi Margherita Zaupa Suphansa Sawamiphak Alessandro Filosa |
| author_sort | Laura Corradi |
| collection | DOAJ |
| description | Summary: The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated or inhibited by the stressor using a relatively rapid immunofluorescence staining approach. This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation.For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T04:46:41Z |
| format | Article |
| id | doaj.art-1bda4504019f4c3da919bcf14da88f32 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T04:46:41Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1bda4504019f4c3da919bcf14da88f322022-12-22T03:01:49ZengElsevierSTAR Protocols2666-16672022-12-0134101731Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvaeLaura Corradi0Margherita Zaupa1Suphansa Sawamiphak2Alessandro Filosa3Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Freie Universität Berlin, Institute for Biology, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Freie Universität Berlin, Institute for Biology, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Corresponding authorSummary: The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated or inhibited by the stressor using a relatively rapid immunofluorescence staining approach. This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation.For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006116MicroscopyModel organismsNeuroscienceBehavior |
| spellingShingle | Laura Corradi Margherita Zaupa Suphansa Sawamiphak Alessandro Filosa Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae STAR Protocols Microscopy Model organisms Neuroscience Behavior |
| title | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| title_full | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| title_fullStr | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| title_full_unstemmed | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| title_short | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| title_sort | using perk immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| topic | Microscopy Model organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006116 |
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