Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

Summary: The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are...

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Bibliographic Details
Main Authors: Laura Corradi, Margherita Zaupa, Suphansa Sawamiphak, Alessandro Filosa
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Microscopy
Model organisms
Neuroscience
Behavior
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006116

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