Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri.
DNA methylation plays a vital role in tissue development and differentiation in eukaryotes. Epigenetic studies have been seldom conducted in the extremely diverse and evolutionarily highly successful bilaterian lineage Mollusca. In the present study, we conducted the genome-wide profiling of DNA met...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3891877?pdf=render |
| _version_ | 1818423291760607232 |
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| author | Yan Sun Rui Hou Xiaoteng Fu Changsen Sun Shi Wang Chen Wang Ning Li Lingling Zhang Zhenmin Bao |
| author_facet | Yan Sun Rui Hou Xiaoteng Fu Changsen Sun Shi Wang Chen Wang Ning Li Lingling Zhang Zhenmin Bao |
| author_sort | Yan Sun |
| collection | DOAJ |
| description | DNA methylation plays a vital role in tissue development and differentiation in eukaryotes. Epigenetic studies have been seldom conducted in the extremely diverse and evolutionarily highly successful bilaterian lineage Mollusca. In the present study, we conducted the genome-wide profiling of DNA methylation for five tissues of a bivalve mollusc, Chlamys farreri using the methylation-sensitive amplification polymorphism (MSAP) technique. The methylation levels were quite similar among tissues, ranging from 20.9% to 21.7%. CG methylation was the dominant type (14.9%-16.5%) in the C. farreri genome, but CHG methylation also accounted for a substantial fraction of total methylation (5.1%-6.3%). Relatively high methylation diversity was observed within tissues. Methylation differentiation between tissues was evaluated and 460 tissue-specific epiloci were identified. Kidney differs from the other tissues in DNA methylation profiles. Our study presents the first look at the tissue-specific DNA methylation patterns in a bivalve mollusc and represents an initial step towards understanding of epigenetic regulatory mechanism underlying tissue development and differentiation in bivalves. |
| first_indexed | 2024-12-14T13:39:49Z |
| format | Article |
| id | doaj.art-1bdad0f1806846f99cafb7dc4e955143 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T13:39:49Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-1bdad0f1806846f99cafb7dc4e9551432022-12-21T22:59:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8623210.1371/journal.pone.0086232Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri.Yan SunRui HouXiaoteng FuChangsen SunShi WangChen WangNing LiLingling ZhangZhenmin BaoDNA methylation plays a vital role in tissue development and differentiation in eukaryotes. Epigenetic studies have been seldom conducted in the extremely diverse and evolutionarily highly successful bilaterian lineage Mollusca. In the present study, we conducted the genome-wide profiling of DNA methylation for five tissues of a bivalve mollusc, Chlamys farreri using the methylation-sensitive amplification polymorphism (MSAP) technique. The methylation levels were quite similar among tissues, ranging from 20.9% to 21.7%. CG methylation was the dominant type (14.9%-16.5%) in the C. farreri genome, but CHG methylation also accounted for a substantial fraction of total methylation (5.1%-6.3%). Relatively high methylation diversity was observed within tissues. Methylation differentiation between tissues was evaluated and 460 tissue-specific epiloci were identified. Kidney differs from the other tissues in DNA methylation profiles. Our study presents the first look at the tissue-specific DNA methylation patterns in a bivalve mollusc and represents an initial step towards understanding of epigenetic regulatory mechanism underlying tissue development and differentiation in bivalves.http://europepmc.org/articles/PMC3891877?pdf=render |
| spellingShingle | Yan Sun Rui Hou Xiaoteng Fu Changsen Sun Shi Wang Chen Wang Ning Li Lingling Zhang Zhenmin Bao Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. PLoS ONE |
| title | Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. |
| title_full | Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. |
| title_fullStr | Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. |
| title_full_unstemmed | Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. |
| title_short | Genome-wide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. |
| title_sort | genome wide analysis of dna methylation in five tissues of zhikong scallop chlamys farreri |
| url | http://europepmc.org/articles/PMC3891877?pdf=render |
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