Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil
Squalene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin bri...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2021-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/full_html/2021/04/bioconf_ppsis2021_03007/bioconf_ppsis2021_03007.html |
| _version_ | 1819081525796274176 |
|---|---|
| author | Dubrovskaya Irina Gerasimenko Evgeniy Slobodyanik Margarita Sonin Sergey |
| author_facet | Dubrovskaya Irina Gerasimenko Evgeniy Slobodyanik Margarita Sonin Sergey |
| author_sort | Dubrovskaya Irina |
| collection | DOAJ |
| description | Squalene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin brief. Sunflower oil distillation cuts have been selected as the subject of the study, since they area promising secondary raw material for the industrial squalene production. The methods of sample preparation and quantification of squalene in sunflower oil distillation cuts applying gas chromatography in combination with mass spectrometry have been adapted.
The aim of the studyis to create an integrated approach to determining the qualitative and quantitative content of squalene in distillation cuts of vegetable oils. To achieve the goal of the study, the following tasks have been solved:
– Amethod of sample preparation of distillation cuts for determination of squalene has been adapted;
– A method of qualitative and quantitative determination of squalene in distillation cuts has been modified.
As a result of this study, a technique for sample preparation of distillation cuts was proposed as well as a method for the qualitative and quantitative (absolute calibrationmethod) determination of squalene in distillation cuts of sunflower oil. To implement the technique, a Kristall 5000 gas chromatograph equipped with a mass spectrometric detector was used. Squalene and background components were recorded using the NIST 11 mass spectral database. |
| first_indexed | 2024-12-21T20:02:10Z |
| format | Article |
| id | doaj.art-1bdd295aabc54a0e8a5c07d9d7ce7236 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-12-21T20:02:10Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-1bdd295aabc54a0e8a5c07d9d7ce72362022-12-21T18:51:56ZengEDP SciencesBIO Web of Conferences2117-44582021-01-01320300710.1051/bioconf/20213203007bioconf_ppsis2021_03007Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower OilDubrovskaya Irina0Gerasimenko Evgeniy1Slobodyanik Margarita2Sonin Sergey3Kuban State Technological UniversityKuban State Technological UniversityKuban State Technological UniversityKuban State Technological UniversitySqualene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin brief. Sunflower oil distillation cuts have been selected as the subject of the study, since they area promising secondary raw material for the industrial squalene production. The methods of sample preparation and quantification of squalene in sunflower oil distillation cuts applying gas chromatography in combination with mass spectrometry have been adapted. The aim of the studyis to create an integrated approach to determining the qualitative and quantitative content of squalene in distillation cuts of vegetable oils. To achieve the goal of the study, the following tasks have been solved: – Amethod of sample preparation of distillation cuts for determination of squalene has been adapted; – A method of qualitative and quantitative determination of squalene in distillation cuts has been modified. As a result of this study, a technique for sample preparation of distillation cuts was proposed as well as a method for the qualitative and quantitative (absolute calibrationmethod) determination of squalene in distillation cuts of sunflower oil. To implement the technique, a Kristall 5000 gas chromatograph equipped with a mass spectrometric detector was used. Squalene and background components were recorded using the NIST 11 mass spectral database.https://www.bio-conferences.org/articles/bioconf/full_html/2021/04/bioconf_ppsis2021_03007/bioconf_ppsis2021_03007.html |
| spellingShingle | Dubrovskaya Irina Gerasimenko Evgeniy Slobodyanik Margarita Sonin Sergey Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil BIO Web of Conferences |
| title | Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil |
| title_full | Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil |
| title_fullStr | Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil |
| title_full_unstemmed | Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil |
| title_short | Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil |
| title_sort | adapting of a method for qualitative and quantitative determination of squalene in distillation cuts of sunflower oil |
| url | https://www.bio-conferences.org/articles/bioconf/full_html/2021/04/bioconf_ppsis2021_03007/bioconf_ppsis2021_03007.html |
| work_keys_str_mv | AT dubrovskayairina adaptingofamethodforqualitativeandquantitativedeterminationofsqualeneindistillationcutsofsunfloweroil AT gerasimenkoevgeniy adaptingofamethodforqualitativeandquantitativedeterminationofsqualeneindistillationcutsofsunfloweroil AT slobodyanikmargarita adaptingofamethodforqualitativeandquantitativedeterminationofsqualeneindistillationcutsofsunfloweroil AT soninsergey adaptingofamethodforqualitativeandquantitativedeterminationofsqualeneindistillationcutsofsunfloweroil |