Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion
Phragmites is the tallest energy crop found as an invasive species worldwide and considered as waste biomass. The present study evaluated the potential of the aquatic biomass Phragmites karka grown from two different lakes as feedstock for biofuel production. A comparative study of biomass was condu...
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Frontiers Media S.A.
2021-04-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenrg.2020.594452/full |
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| author | Sabeela Beevi Ummalyma Dinabandhu Sahoo Anoop Pudiyamadam Velayudhanpillai Prasannakumari Adarsh Rajeev K. Sukumaran Thallada Bhaskar Thallada Bhaskar Ajay Parida |
| author_facet | Sabeela Beevi Ummalyma Dinabandhu Sahoo Anoop Pudiyamadam Velayudhanpillai Prasannakumari Adarsh Rajeev K. Sukumaran Thallada Bhaskar Thallada Bhaskar Ajay Parida |
| author_sort | Sabeela Beevi Ummalyma |
| collection | DOAJ |
| description | Phragmites is the tallest energy crop found as an invasive species worldwide and considered as waste biomass. The present study evaluated the potential of the aquatic biomass Phragmites karka grown from two different lakes as feedstock for biofuel production. A comparative study of biomass was conducted from Chilika Lake and Loktak Lake, India. The methodology involves dilute acid, sono-assisted alkali pretreatment, and hydrolyzed biomass with commercial cellulase. SEM, XRD, and FTIR analysis were performed for the biomass physicochemical studies and confirmed that alterations occurred in the biomass structure, assisting the hydrolysis process. The enzymatic hydrolysis result showed that the highest of reducing sugar yield of 79% was obtained from biomass loading of 10% and 1% w/v alkali with a sonication frequency of 20 kHz for 25 min. Acid pretreatment released maximal reducing sugar yield of 73% attained from biomass loading of 20% and 0.5% w/v acid. Composition analysis of biomass showed that cellulose content increased from 36% to 46%. Sono-assisted alkali pretreatment solubilized 40% of lignin content compared to untreated biomass. Final ethanol recovery from the biomass is 78% fermentation efficiency from glucose. The data indicate that exploiting tall reed grass as a bioenergy raw material can be a viable approach for sustainable utilization of invasive grass/waste biomass for biorefineries, which helps control invasive weeds and management of waste. |
| first_indexed | 2024-12-22T13:25:48Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-22T13:25:48Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Energy Research |
| spelling | doaj.art-1be0757915c7422fb5666165646b91602022-12-21T18:24:18ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2021-04-01810.3389/fenrg.2020.594452594452Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol ConversionSabeela Beevi Ummalyma0Dinabandhu Sahoo1Anoop Pudiyamadam2Velayudhanpillai Prasannakumari Adarsh3Rajeev K. Sukumaran4Thallada Bhaskar5Thallada Bhaskar6Ajay Parida7Institute of Bioresources and Sustainable Development (IBSD), A National Institute under Department of Biotechnology, Govt of India Takyelpat, Imphal, IndiaDepartment of Botany, University of Delhi, Delhi, IndiaMicrobial processes and Technology Division, Centre for Biofuels National Institute for Interdisciplinary Science and Technology-CSIR, Trivandrum, IndiaMicrobial processes and Technology Division, Centre for Biofuels National Institute for Interdisciplinary Science and Technology-CSIR, Trivandrum, IndiaMicrobial processes and Technology Division, Centre for Biofuels National Institute for Interdisciplinary Science and Technology-CSIR, Trivandrum, IndiaThermo-Catalytic Processes Area (TPA), Material Resource Efficiency Division, Indian Institute of Petroleum-CSIR, Dehradun, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaInstitute of Life Sciences, NALCO Square, Bhubaneswar, Odisha, IndiaPhragmites is the tallest energy crop found as an invasive species worldwide and considered as waste biomass. The present study evaluated the potential of the aquatic biomass Phragmites karka grown from two different lakes as feedstock for biofuel production. A comparative study of biomass was conducted from Chilika Lake and Loktak Lake, India. The methodology involves dilute acid, sono-assisted alkali pretreatment, and hydrolyzed biomass with commercial cellulase. SEM, XRD, and FTIR analysis were performed for the biomass physicochemical studies and confirmed that alterations occurred in the biomass structure, assisting the hydrolysis process. The enzymatic hydrolysis result showed that the highest of reducing sugar yield of 79% was obtained from biomass loading of 10% and 1% w/v alkali with a sonication frequency of 20 kHz for 25 min. Acid pretreatment released maximal reducing sugar yield of 73% attained from biomass loading of 20% and 0.5% w/v acid. Composition analysis of biomass showed that cellulose content increased from 36% to 46%. Sono-assisted alkali pretreatment solubilized 40% of lignin content compared to untreated biomass. Final ethanol recovery from the biomass is 78% fermentation efficiency from glucose. The data indicate that exploiting tall reed grass as a bioenergy raw material can be a viable approach for sustainable utilization of invasive grass/waste biomass for biorefineries, which helps control invasive weeds and management of waste.https://www.frontiersin.org/articles/10.3389/fenrg.2020.594452/fullPhragmitessonicationpretreatmentChilika LakeLoktak lake |
| spellingShingle | Sabeela Beevi Ummalyma Dinabandhu Sahoo Anoop Pudiyamadam Velayudhanpillai Prasannakumari Adarsh Rajeev K. Sukumaran Thallada Bhaskar Thallada Bhaskar Ajay Parida Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion Frontiers in Energy Research Phragmites sonication pretreatment Chilika Lake Loktak lake |
| title | Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion |
| title_full | Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion |
| title_fullStr | Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion |
| title_full_unstemmed | Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion |
| title_short | Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion |
| title_sort | sono assisted alkali and dilute acid pretreatment of phragmites karka tall reed grass to enhance enzymatic digestibility for bioethanol conversion |
| topic | Phragmites sonication pretreatment Chilika Lake Loktak lake |
| url | https://www.frontiersin.org/articles/10.3389/fenrg.2020.594452/full |
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