Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers
Breast cancer is the second leading cause of cancer deaths in women worldwide; therefore, there is an increased need for the discovery, development, optimization, and quantification of diagnostic biomarkers that can improve the disease diagnosis, prognosis, and therapeutic outcome. Circulating cell-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/8/4128 |
| _version_ | 1827743667344900096 |
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| author | Ana-Maria Chiorcea-Paquim |
| author_facet | Ana-Maria Chiorcea-Paquim |
| author_sort | Ana-Maria Chiorcea-Paquim |
| collection | DOAJ |
| description | Breast cancer is the second leading cause of cancer deaths in women worldwide; therefore, there is an increased need for the discovery, development, optimization, and quantification of diagnostic biomarkers that can improve the disease diagnosis, prognosis, and therapeutic outcome. Circulating cell-free nucleic acids biomarkers such as microRNAs (miRNAs) and breast cancer susceptibility gene 1 (BRCA1) allow the characterization of the genetic features and screening breast cancer patients. Electrochemical biosensors offer excellent platforms for the detection of breast cancer biomarkers due to their high sensitivity and selectivity, low cost, use of small analyte volumes, and easy miniaturization. In this context, this article provides an exhaustive review concerning the electrochemical methods of characterization and quantification of different miRNAs and BRCA1 breast cancer biomarkers using electrochemical DNA biosensors based on the detection of hybridization events between a DNA or peptide nucleic acid probe and the target nucleic acid sequence. The fabrication approaches, the biosensors architectures, the signal amplification strategies, the detection techniques, and the key performance parameters, such as the linearity range and the limit of detection, were discussed. |
| first_indexed | 2024-03-11T04:32:43Z |
| format | Article |
| id | doaj.art-1be51739ea4b4957a336b873c70dca38 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T04:32:43Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1be51739ea4b4957a336b873c70dca382023-11-17T21:19:28ZengMDPI AGSensors1424-82202023-04-01238412810.3390/s23084128Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer BiomarkersAna-Maria Chiorcea-Paquim0University of Coimbra, CEMMPRE, ARISE, Department of Chemistry, 3004-535 Coimbra, PortugalBreast cancer is the second leading cause of cancer deaths in women worldwide; therefore, there is an increased need for the discovery, development, optimization, and quantification of diagnostic biomarkers that can improve the disease diagnosis, prognosis, and therapeutic outcome. Circulating cell-free nucleic acids biomarkers such as microRNAs (miRNAs) and breast cancer susceptibility gene 1 (BRCA1) allow the characterization of the genetic features and screening breast cancer patients. Electrochemical biosensors offer excellent platforms for the detection of breast cancer biomarkers due to their high sensitivity and selectivity, low cost, use of small analyte volumes, and easy miniaturization. In this context, this article provides an exhaustive review concerning the electrochemical methods of characterization and quantification of different miRNAs and BRCA1 breast cancer biomarkers using electrochemical DNA biosensors based on the detection of hybridization events between a DNA or peptide nucleic acid probe and the target nucleic acid sequence. The fabrication approaches, the biosensors architectures, the signal amplification strategies, the detection techniques, and the key performance parameters, such as the linearity range and the limit of detection, were discussed.https://www.mdpi.com/1424-8220/23/8/4128cancer biomarkerbreast cancermicroRNAmi-RNAmiR-21miR-155 |
| spellingShingle | Ana-Maria Chiorcea-Paquim Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers Sensors cancer biomarker breast cancer microRNA mi-RNA miR-21 miR-155 |
| title | Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers |
| title_full | Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers |
| title_fullStr | Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers |
| title_full_unstemmed | Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers |
| title_short | Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers |
| title_sort | advances in electrochemical biosensor technologies for the detection of nucleic acid breast cancer biomarkers |
| topic | cancer biomarker breast cancer microRNA mi-RNA miR-21 miR-155 |
| url | https://www.mdpi.com/1424-8220/23/8/4128 |
| work_keys_str_mv | AT anamariachiorceapaquim advancesinelectrochemicalbiosensortechnologiesforthedetectionofnucleicacidbreastcancerbiomarkers |