An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid
Abstract Background Ecosystems are brimming with myriad compounds, including some at very low concentrations that are indispensable for insect survival and reproduction. Screening strategies for identifying active compounds are typically based on bioassay-guided approaches. Results Here, we selected...
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| Format: | Article |
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BMC
2024-03-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-024-01862-9 |
| _version_ | 1797258988827443200 |
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| author | Yipeng Liu Sai Zhang Song Cao Emmanuelle Jacquin-Joly Qiong Zhou Yang Liu Guirong Wang |
| author_facet | Yipeng Liu Sai Zhang Song Cao Emmanuelle Jacquin-Joly Qiong Zhou Yang Liu Guirong Wang |
| author_sort | Yipeng Liu |
| collection | DOAJ |
| description | Abstract Background Ecosystems are brimming with myriad compounds, including some at very low concentrations that are indispensable for insect survival and reproduction. Screening strategies for identifying active compounds are typically based on bioassay-guided approaches. Results Here, we selected two candidate odorant receptors from a major pest of cruciferous plants—the diamondback moth Plutella xylostella—as targets to screen for active semiochemicals. One of these ORs, PxylOR16, exhibited a specific, sensitive response to heptanal, with both larvae and adult P. xylostella displaying heptanal avoidance behavior. Gene knockout studies based on CRISPR/Cas9 experimentally confirmed that PxylOR16 mediates this avoidance. Intriguingly, rather than being involved in P. xylostella–host plant interaction, we discovered that P. xylostella recognizes heptanal from the cuticular volatiles of the parasitoid wasp Cotesia vestalis, possibly to avoid parasitization. Conclusions Our study thus showcases how the deorphanization of odorant receptors can drive discoveries about their complex functions in mediating insect survival. We also demonstrate that the use of odorant receptors as a screening platform could be efficient in identifying new behavioral regulators for application in pest management. |
| first_indexed | 2024-04-24T23:02:17Z |
| format | Article |
| id | doaj.art-1be8118e2a0b4745a20f8328dba917dd |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-04-24T23:02:17Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-1be8118e2a0b4745a20f8328dba917dd2024-03-17T12:38:25ZengBMCBMC Biology1741-70072024-03-0122111610.1186/s12915-024-01862-9An odorant receptor mediates the avoidance of Plutella xylostella against parasitoidYipeng Liu0Sai Zhang1Song Cao2Emmanuelle Jacquin-Joly3Qiong Zhou4Yang Liu5Guirong Wang6State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesInstitute of Ecology and Environmental Sciences of Paris, INRAE, Sorbonne Université, CNRS, UPEC, UniversitéParis CitéCollege of Life Sciences, Hunan Normal UniversityState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAbstract Background Ecosystems are brimming with myriad compounds, including some at very low concentrations that are indispensable for insect survival and reproduction. Screening strategies for identifying active compounds are typically based on bioassay-guided approaches. Results Here, we selected two candidate odorant receptors from a major pest of cruciferous plants—the diamondback moth Plutella xylostella—as targets to screen for active semiochemicals. One of these ORs, PxylOR16, exhibited a specific, sensitive response to heptanal, with both larvae and adult P. xylostella displaying heptanal avoidance behavior. Gene knockout studies based on CRISPR/Cas9 experimentally confirmed that PxylOR16 mediates this avoidance. Intriguingly, rather than being involved in P. xylostella–host plant interaction, we discovered that P. xylostella recognizes heptanal from the cuticular volatiles of the parasitoid wasp Cotesia vestalis, possibly to avoid parasitization. Conclusions Our study thus showcases how the deorphanization of odorant receptors can drive discoveries about their complex functions in mediating insect survival. We also demonstrate that the use of odorant receptors as a screening platform could be efficient in identifying new behavioral regulators for application in pest management.https://doi.org/10.1186/s12915-024-01862-9Plutella xylostellaOdorant receptorHeptanalAvoidance behaviorParasitoid wasp |
| spellingShingle | Yipeng Liu Sai Zhang Song Cao Emmanuelle Jacquin-Joly Qiong Zhou Yang Liu Guirong Wang An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid BMC Biology Plutella xylostella Odorant receptor Heptanal Avoidance behavior Parasitoid wasp |
| title | An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid |
| title_full | An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid |
| title_fullStr | An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid |
| title_full_unstemmed | An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid |
| title_short | An odorant receptor mediates the avoidance of Plutella xylostella against parasitoid |
| title_sort | odorant receptor mediates the avoidance of plutella xylostella against parasitoid |
| topic | Plutella xylostella Odorant receptor Heptanal Avoidance behavior Parasitoid wasp |
| url | https://doi.org/10.1186/s12915-024-01862-9 |
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