Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense
Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), especially tropical race 4 (TR4), presents the foremost menace to the global banana production. Extensive efforts have been made to search for efficient biological control agents for disease management. Our previous st...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1177393/full |
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| author | Xiaxia Wang Xiaxia Wang Zhenghua Du Zhenghua Du Chanxin Chen Chanxin Chen Shuang Guo Shuang Guo Qianzhuo Mao Wei Wu Ruimei Wu Wenbo Han Peifeng Xie Yiping Zeng Wenna Shan Zonghua Wang Zonghua Wang Xiaomin Yu |
| author_facet | Xiaxia Wang Xiaxia Wang Zhenghua Du Zhenghua Du Chanxin Chen Chanxin Chen Shuang Guo Shuang Guo Qianzhuo Mao Wei Wu Ruimei Wu Wenbo Han Peifeng Xie Yiping Zeng Wenna Shan Zonghua Wang Zonghua Wang Xiaomin Yu |
| author_sort | Xiaxia Wang |
| collection | DOAJ |
| description | Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), especially tropical race 4 (TR4), presents the foremost menace to the global banana production. Extensive efforts have been made to search for efficient biological control agents for disease management. Our previous study showed that Streptomyces sp. XY006 exhibited a strong inhibitory activity against several phytopathogenic fungi, including F. oxysporum. Here, the corresponding antifungal metabolites were purified and determined to be two cyclic lipopeptide homologs, lipopeptin A and lipopeptin B. Combined treatment with lipopeptin complex antagonized Foc TR4 by inhibiting mycelial growth and conidial sporulation, suppressing the synthesis of ergosterol and fatty acids and lowering the production of fusaric acid. Electron microscopy observation showed that lipopeptide treatment induced a severe disruption of the plasma membrane, leading to cell leakage. Lipopeptin A displayed a more pronounced antifungal activity against Foc TR4 than lipopeptin B. In pot experiments, strain XY006 successfully colonized banana plantlets and suppressed the incidence of FWB, with a biocontrol efficacy of up to 87.7%. Additionally, XY006 fermentation culture application improved plant growth parameters and induced peroxidase activity in treated plantlets, suggesting a possible role in induced resistance. Our findings highlight the potential of strain XY006 as a biological agent for FWB, and further research is needed to enhance its efficacy and mode of action in planta. |
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| language | English |
| last_indexed | 2024-04-09T15:42:43Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-1be9108dafcb4dd385550909c85d823d2023-04-27T05:35:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11773931177393Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubenseXiaxia Wang0Xiaxia Wang1Zhenghua Du2Zhenghua Du3Chanxin Chen4Chanxin Chen5Shuang Guo6Shuang Guo7Qianzhuo Mao8Wei Wu9Ruimei Wu10Wenbo Han11Peifeng Xie12Yiping Zeng13Wenna Shan14Zonghua Wang15Zonghua Wang16Xiaomin Yu17State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaSchool of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaSchool of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), especially tropical race 4 (TR4), presents the foremost menace to the global banana production. Extensive efforts have been made to search for efficient biological control agents for disease management. Our previous study showed that Streptomyces sp. XY006 exhibited a strong inhibitory activity against several phytopathogenic fungi, including F. oxysporum. Here, the corresponding antifungal metabolites were purified and determined to be two cyclic lipopeptide homologs, lipopeptin A and lipopeptin B. Combined treatment with lipopeptin complex antagonized Foc TR4 by inhibiting mycelial growth and conidial sporulation, suppressing the synthesis of ergosterol and fatty acids and lowering the production of fusaric acid. Electron microscopy observation showed that lipopeptide treatment induced a severe disruption of the plasma membrane, leading to cell leakage. Lipopeptin A displayed a more pronounced antifungal activity against Foc TR4 than lipopeptin B. In pot experiments, strain XY006 successfully colonized banana plantlets and suppressed the incidence of FWB, with a biocontrol efficacy of up to 87.7%. Additionally, XY006 fermentation culture application improved plant growth parameters and induced peroxidase activity in treated plantlets, suggesting a possible role in induced resistance. Our findings highlight the potential of strain XY006 as a biological agent for FWB, and further research is needed to enhance its efficacy and mode of action in planta.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1177393/fullantifungal effectStreptomycesbanana Fusarium wiltlipopeptidebiocontrol |
| spellingShingle | Xiaxia Wang Xiaxia Wang Zhenghua Du Zhenghua Du Chanxin Chen Chanxin Chen Shuang Guo Shuang Guo Qianzhuo Mao Wei Wu Ruimei Wu Wenbo Han Peifeng Xie Yiping Zeng Wenna Shan Zonghua Wang Zonghua Wang Xiaomin Yu Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense Frontiers in Microbiology antifungal effect Streptomyces banana Fusarium wilt lipopeptide biocontrol |
| title | Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense |
| title_full | Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense |
| title_fullStr | Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense |
| title_full_unstemmed | Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense |
| title_short | Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense |
| title_sort | antifungal effects and biocontrol potential of lipopeptide producing streptomyces against banana fusarium wilt fungus fusarium oxysporum f sp cubense |
| topic | antifungal effect Streptomyces banana Fusarium wilt lipopeptide biocontrol |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1177393/full |
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