Toxoplasma gondii Chitinase Induces Macrophage Activation.
Toxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that is able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential to various biological processes, and some pathogens rely on chitinases for successful parasiti...
| Glavni autori: | , , , , , , , , , , , , |
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| Format: | Članak |
| Jezik: | English |
| Izdano: |
Public Library of Science (PLoS)
2015-01-01
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| Serija: | PLoS ONE |
| Online pristup: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144507&type=printable |
| _version_ | 1826584227112550400 |
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| author | Fausto Almeida Aline Sardinha-Silva Thiago Aparecido da Silva André Moreira Pessoni Camila Figueiredo Pinzan Ana Claudia Paiva Alegre-Maller Nerry Tatiana Cecílio Nilmar Silvio Moretti André Ricardo Lima Damásio Wellington Ramos Pedersoli José Roberto Mineo Roberto Nascimento Silva Maria Cristina Roque-Barreira |
| author_facet | Fausto Almeida Aline Sardinha-Silva Thiago Aparecido da Silva André Moreira Pessoni Camila Figueiredo Pinzan Ana Claudia Paiva Alegre-Maller Nerry Tatiana Cecílio Nilmar Silvio Moretti André Ricardo Lima Damásio Wellington Ramos Pedersoli José Roberto Mineo Roberto Nascimento Silva Maria Cristina Roque-Barreira |
| author_sort | Fausto Almeida |
| collection | DOAJ |
| description | Toxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that is able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential to various biological processes, and some pathogens rely on chitinases for successful parasitization. Here, we purified and characterized a chitinase from T. gondii. The enzyme, provisionally named Tg_chitinase, has a molecular mass of 13.7 kDa and exhibits a Km of 0.34 mM and a Vmax of 2.64. The optimal environmental conditions for enzymatic function were at pH 4.0 and 50 °C. Tg_chitinase was immunolocalized in the cytoplasm of highly virulent T. gondii RH strain tachyzoites, mainly at the apical extremity. Tg_chitinase induced macrophage activation as manifested by the production of high levels of pro-inflammatory cytokines, a pathogenic hallmark of T. gondii infection. In conclusion, to our knowledge, we describe for the first time a chitinase of T. gondii tachyzoites and provide evidence that this enzyme might influence the pathogenesis of T. gondii infection. |
| first_indexed | 2024-04-12T19:20:05Z |
| format | Article |
| id | doaj.art-1be9906d97294235932c6db185d58f5e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:34:24Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1be9906d97294235932c6db185d58f5e2025-02-25T05:33:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014450710.1371/journal.pone.0144507Toxoplasma gondii Chitinase Induces Macrophage Activation.Fausto AlmeidaAline Sardinha-SilvaThiago Aparecido da SilvaAndré Moreira PessoniCamila Figueiredo PinzanAna Claudia Paiva Alegre-MallerNerry Tatiana CecílioNilmar Silvio MorettiAndré Ricardo Lima DamásioWellington Ramos PedersoliJosé Roberto MineoRoberto Nascimento SilvaMaria Cristina Roque-BarreiraToxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that is able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential to various biological processes, and some pathogens rely on chitinases for successful parasitization. Here, we purified and characterized a chitinase from T. gondii. The enzyme, provisionally named Tg_chitinase, has a molecular mass of 13.7 kDa and exhibits a Km of 0.34 mM and a Vmax of 2.64. The optimal environmental conditions for enzymatic function were at pH 4.0 and 50 °C. Tg_chitinase was immunolocalized in the cytoplasm of highly virulent T. gondii RH strain tachyzoites, mainly at the apical extremity. Tg_chitinase induced macrophage activation as manifested by the production of high levels of pro-inflammatory cytokines, a pathogenic hallmark of T. gondii infection. In conclusion, to our knowledge, we describe for the first time a chitinase of T. gondii tachyzoites and provide evidence that this enzyme might influence the pathogenesis of T. gondii infection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144507&type=printable |
| spellingShingle | Fausto Almeida Aline Sardinha-Silva Thiago Aparecido da Silva André Moreira Pessoni Camila Figueiredo Pinzan Ana Claudia Paiva Alegre-Maller Nerry Tatiana Cecílio Nilmar Silvio Moretti André Ricardo Lima Damásio Wellington Ramos Pedersoli José Roberto Mineo Roberto Nascimento Silva Maria Cristina Roque-Barreira Toxoplasma gondii Chitinase Induces Macrophage Activation. PLoS ONE |
| title | Toxoplasma gondii Chitinase Induces Macrophage Activation. |
| title_full | Toxoplasma gondii Chitinase Induces Macrophage Activation. |
| title_fullStr | Toxoplasma gondii Chitinase Induces Macrophage Activation. |
| title_full_unstemmed | Toxoplasma gondii Chitinase Induces Macrophage Activation. |
| title_short | Toxoplasma gondii Chitinase Induces Macrophage Activation. |
| title_sort | toxoplasma gondii chitinase induces macrophage activation |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144507&type=printable |
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