The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery
Nan Zhang, Lei Ma, Wenyuan Ding Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of ChinaCorrespondence: Wenyuan Ding, Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050051, People&a...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-01-01
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| Series: | International Journal of General Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-diagnostic-value-of-blood-next-generation-sequencing-in-early-surg-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1797955533482754048 |
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| author | Zhang N Ma L Ding W |
| author_facet | Zhang N Ma L Ding W |
| author_sort | Zhang N |
| collection | DOAJ |
| description | Nan Zhang, Lei Ma, Wenyuan Ding Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of ChinaCorrespondence: Wenyuan Ding, Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050051, People’s Republic of China, Email zhangnan_5867@163.comObjective: To determine the diagnostic value of blood next-generation sequencing (NGS) in early surgical site infection after spine surgery. Because the blood is sterile in healthy individuals, it is expected that blood NGS is both sensitive and specific for the detection of infection.Methods: A total of 28 patients with definitive spinal surgical site infections and controls (n=30) were retrospectively included. The postoperative results of NGS and culture on different samples, such as blood and drainage fluid, were obtained and compared to evaluate the diagnostic value of blood NGS. The diagnostic value parameters (sensitivity, specificity, etc.) were calculated.Results: Among the four bacteriological exam methods, blood NGS was both sensitive and specific for the determination of infection after spine surgery. The sensitivities of blood and drainage fluid NGS were similar (0.82 vs 0.89, P=0.617). However, the specificities of the two assessments differed, which were 0.97 for blood NGS and 0.40 for drainage fluid NGS (P< 0.001). The sensitivities of bacterial culture were lower than those of NGS (blood: 0.82 vs 0.25, P< 0.001; drainage fluid: 0.89 vs 0.61, P< 0.001), regardless of the sample type. However, the specificities of bacterial culture were equal to or higher than those of NGS (blood: 0.97 vs 0.97, P=1.000; drainage fluid: 0.40 vs 0.80, P=0.002).Conclusion: This article emphasizes the superiority of blood NGS in infection detection and bacterial determination in patients undergoing spine surgery. Compared with traditional drainage fluid bacterial culture and NGS, blood NGS was more sensitive and specific, and its extensive application could be expected.Keywords: next-generation sequencing, diagnostic test, surgical site infection, spine surgery |
| first_indexed | 2024-04-10T23:35:48Z |
| format | Article |
| id | doaj.art-1bec2e6cc09543109d6f8c2a440abcc4 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-04-10T23:35:48Z |
| publishDate | 2023-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-1bec2e6cc09543109d6f8c2a440abcc42023-01-11T19:18:14ZengDove Medical PressInternational Journal of General Medicine1178-70742023-01-01Volume 16374580786The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine SurgeryZhang NMa LDing WNan Zhang, Lei Ma, Wenyuan Ding Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of ChinaCorrespondence: Wenyuan Ding, Department of Spine Surgery, 3rd Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050051, People’s Republic of China, Email zhangnan_5867@163.comObjective: To determine the diagnostic value of blood next-generation sequencing (NGS) in early surgical site infection after spine surgery. Because the blood is sterile in healthy individuals, it is expected that blood NGS is both sensitive and specific for the detection of infection.Methods: A total of 28 patients with definitive spinal surgical site infections and controls (n=30) were retrospectively included. The postoperative results of NGS and culture on different samples, such as blood and drainage fluid, were obtained and compared to evaluate the diagnostic value of blood NGS. The diagnostic value parameters (sensitivity, specificity, etc.) were calculated.Results: Among the four bacteriological exam methods, blood NGS was both sensitive and specific for the determination of infection after spine surgery. The sensitivities of blood and drainage fluid NGS were similar (0.82 vs 0.89, P=0.617). However, the specificities of the two assessments differed, which were 0.97 for blood NGS and 0.40 for drainage fluid NGS (P< 0.001). The sensitivities of bacterial culture were lower than those of NGS (blood: 0.82 vs 0.25, P< 0.001; drainage fluid: 0.89 vs 0.61, P< 0.001), regardless of the sample type. However, the specificities of bacterial culture were equal to or higher than those of NGS (blood: 0.97 vs 0.97, P=1.000; drainage fluid: 0.40 vs 0.80, P=0.002).Conclusion: This article emphasizes the superiority of blood NGS in infection detection and bacterial determination in patients undergoing spine surgery. Compared with traditional drainage fluid bacterial culture and NGS, blood NGS was more sensitive and specific, and its extensive application could be expected.Keywords: next-generation sequencing, diagnostic test, surgical site infection, spine surgeryhttps://www.dovepress.com/the-diagnostic-value-of-blood-next-generation-sequencing-in-early-surg-peer-reviewed-fulltext-article-IJGMnext-generation sequencingdiagnostic testsurgical site infectionspine surgery |
| spellingShingle | Zhang N Ma L Ding W The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery International Journal of General Medicine next-generation sequencing diagnostic test surgical site infection spine surgery |
| title | The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery |
| title_full | The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery |
| title_fullStr | The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery |
| title_full_unstemmed | The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery |
| title_short | The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery |
| title_sort | diagnostic value of blood next generation sequencing in early surgical site infection after spine surgery |
| topic | next-generation sequencing diagnostic test surgical site infection spine surgery |
| url | https://www.dovepress.com/the-diagnostic-value-of-blood-next-generation-sequencing-in-early-surg-peer-reviewed-fulltext-article-IJGM |
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