Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes
The enantioselective synthesis of organic compounds is one of the great challenges in organic synthetic chemistry due to its importance for the acquisition of biologically active derivatives, e.g., pharmaceuticals, agrochemicals, and others. This is why biological systems are increasingly applied as...
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MDPI AG
2021-06-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/11/7/781 |
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| author | Agnieszka Raczyńska Joanna Jadczyk Małgorzata Brzezińska-Rodak |
| author_facet | Agnieszka Raczyńska Joanna Jadczyk Małgorzata Brzezińska-Rodak |
| author_sort | Agnieszka Raczyńska |
| collection | DOAJ |
| description | The enantioselective synthesis of organic compounds is one of the great challenges in organic synthetic chemistry due to its importance for the acquisition of biologically active derivatives, e.g., pharmaceuticals, agrochemicals, and others. This is why biological systems are increasingly applied as tools for chiral compounds synthesis or modification. The use of whole cells of “wild-type” microorganisms is one possible approach, especially as some methods allow improving the conversion degrees and controlling the stereoselectivity of the reaction without the need to introduce changes at the genetic level. Simple manipulation of the culture conditions, the form of a biocatalyst, or the appropriate composition of the biotransformation medium makes it possible to obtain optically pure products in a cheap, safe, and environmentally friendly manner. This review contains selected examples of the influence of physicochemical factors on the stereochemistry of the biocatalytic preparation of enantiomerically pure compounds, which is undertaken through kinetically controlled separation of their racemic mixtures or reduction of prochiral ketones and has an effect on the final enantiomeric purity and enantioselectivity of the reaction. |
| first_indexed | 2024-03-10T10:00:21Z |
| format | Article |
| id | doaj.art-1bedc797b6d241228650c43cfac41a06 |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T10:00:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-1bedc797b6d241228650c43cfac41a062023-11-22T01:56:13ZengMDPI AGCatalysts2073-43442021-06-0111778110.3390/catal11070781Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the ProcessesAgnieszka Raczyńska0Joanna Jadczyk1Małgorzata Brzezińska-Rodak2Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, 50-370 Wrocław, PolandDepartment of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, 50-370 Wrocław, PolandDepartment of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, 50-370 Wrocław, PolandThe enantioselective synthesis of organic compounds is one of the great challenges in organic synthetic chemistry due to its importance for the acquisition of biologically active derivatives, e.g., pharmaceuticals, agrochemicals, and others. This is why biological systems are increasingly applied as tools for chiral compounds synthesis or modification. The use of whole cells of “wild-type” microorganisms is one possible approach, especially as some methods allow improving the conversion degrees and controlling the stereoselectivity of the reaction without the need to introduce changes at the genetic level. Simple manipulation of the culture conditions, the form of a biocatalyst, or the appropriate composition of the biotransformation medium makes it possible to obtain optically pure products in a cheap, safe, and environmentally friendly manner. This review contains selected examples of the influence of physicochemical factors on the stereochemistry of the biocatalytic preparation of enantiomerically pure compounds, which is undertaken through kinetically controlled separation of their racemic mixtures or reduction of prochiral ketones and has an effect on the final enantiomeric purity and enantioselectivity of the reaction.https://www.mdpi.com/2073-4344/11/7/781whole-cellbiocatalystenantiomerically pure productsinfluence of external factors |
| spellingShingle | Agnieszka Raczyńska Joanna Jadczyk Małgorzata Brzezińska-Rodak Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes Catalysts whole-cell biocatalyst enantiomerically pure products influence of external factors |
| title | Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes |
| title_full | Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes |
| title_fullStr | Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes |
| title_full_unstemmed | Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes |
| title_short | Altering the Stereoselectivity of Whole-Cell Biotransformations via the Physicochemical Parameters Impacting the Processes |
| title_sort | altering the stereoselectivity of whole cell biotransformations via the physicochemical parameters impacting the processes |
| topic | whole-cell biocatalyst enantiomerically pure products influence of external factors |
| url | https://www.mdpi.com/2073-4344/11/7/781 |
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