Ex situ Conservation of Three Endemic and/or Endangered Dianthus Species
<p><span style="font-size: x-small;">Within the current context of declining biodiversity, the botanical gardens play an essential role in its conservation. </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2013-05-01
|
| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Online Access: | http://www.notulaebotanicae.ro/index.php/nbha/article/view/9110 |
| Summary: | <p><span style="font-size: x-small;">Within the current context of declining biodiversity, the botanical gardens play an essential role in its conservation. </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">Dianthus callizonus</span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">, </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">D. glacialis </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">ssp. </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">gelidus </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">and </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">D. spiculifolius </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">are the species that we seek to preserve in "Alexandru Borza" Botanical Garden of Cluj-Napoca (Romania). Several replicates were collected for each taxon from different populations in order to avoid the genetic uniformity. The material collected from the natural sites, was planted on a rockery, specially designed for this collection in the Botanical Garden. At the time of planting, each individual was sampled for setting up an </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">in vitro </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">collection and further biochemical and molecular analyses. In case of </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">ex situ </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">outdoor conservation of the three </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">Dianthus </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">species, 80.6% of the individuals collected in the field survived during the first year but the percentage decreased drastically after four years. In the case of </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">in situ </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">collected individuals, as well as in the case of </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">in vitro </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">individuals, </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">D. spiculifolius </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">had the best ability to acclimatize in the Botanical Garden, and </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">D. callizonus </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">presented the lowest number of surviving individuals. The </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">ex vitro </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">acclimatization of the plantlets had 80% efficiency at 10ºC, using three different substrates: soil and pearl stone mix 1/1, soil and sand mix 1/1 and pearl stone. All the three species are preserved </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">in vitro, </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">whereas the plantlets are acclimatized outdoors. </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">Ex situ </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"></span><span style="font-size: x-small;">conservation of these species will have a positive impact on the biodiversity conservation.</span></p> |
|---|---|
| ISSN: | 0255-965X 1842-4309 |