Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada
Abstract The worldwide demand for potato production requires the constant development of new potato varieties with improved yield, quality, disease resistance, and abiotic tolerance. However, cultivar registration is preceded by a long process to morphologically and physiologically characterize the...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-06-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.140 |
| _version_ | 1818676060402745344 |
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| author | Anne‐Sophie Tillault Dmytro P. Yevtushenko |
| author_facet | Anne‐Sophie Tillault Dmytro P. Yevtushenko |
| author_sort | Anne‐Sophie Tillault |
| collection | DOAJ |
| description | Abstract The worldwide demand for potato production requires the constant development of new potato varieties with improved yield, quality, disease resistance, and abiotic tolerance. However, cultivar registration is preceded by a long process to morphologically and physiologically characterize the plants. Notably, this process can be expedited by DNA marker analysis. Simple sequence repeats (SSRs), also known as microsatellites, are the most common reliable DNA markers used to discriminate between genotypes. In this study, 20 potato varieties, including five new genotypes developed in Alberta, Canada, were fingerprinted using 10 SSR markers selected for their high discriminatory power. Different SSRs were amplified from potato DNA using specific primers, and the DNA fragment sizes were analyzed by denaturing polyacrylamide gel electrophoresis. The number of alleles per locus ranged from two for the SSR marker STPoAc58 to six for STM0030 and STM0037 with an average of 4.4. In addition, a total of 77 unique patterns were observed for the 10 SSR markers. The polymorphic information content ranged from 0.477 to 0.802 with an average of 0.675 per locus. In this study, STM0037, STM1016, and STM1104 were found to be the best SSR markers to detect genetic differences between potato varieties. A minimum of two markers was required to distinguish between all 20 genotypes. Most importantly, this highly informative molecular tool confirmed that the developed potato varieties were genetically different from their respective maternal lines and potentially constituted new cultivars. |
| first_indexed | 2024-12-17T08:37:28Z |
| format | Article |
| id | doaj.art-1bf1f6295036447295f5665b7b04e9c5 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-17T08:37:28Z |
| publishDate | 2019-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-1bf1f6295036447295f5665b7b04e9c52022-12-21T21:56:26ZengWileyPlant Direct2475-44552019-06-0136n/an/a10.1002/pld3.140Simple sequence repeat analysis of new potato varieties developed in Alberta, CanadaAnne‐Sophie Tillault0Dmytro P. Yevtushenko1Department of Biological Sciences University of Lethbridge Lethbridge Alberta CanadaDepartment of Biological Sciences University of Lethbridge Lethbridge Alberta CanadaAbstract The worldwide demand for potato production requires the constant development of new potato varieties with improved yield, quality, disease resistance, and abiotic tolerance. However, cultivar registration is preceded by a long process to morphologically and physiologically characterize the plants. Notably, this process can be expedited by DNA marker analysis. Simple sequence repeats (SSRs), also known as microsatellites, are the most common reliable DNA markers used to discriminate between genotypes. In this study, 20 potato varieties, including five new genotypes developed in Alberta, Canada, were fingerprinted using 10 SSR markers selected for their high discriminatory power. Different SSRs were amplified from potato DNA using specific primers, and the DNA fragment sizes were analyzed by denaturing polyacrylamide gel electrophoresis. The number of alleles per locus ranged from two for the SSR marker STPoAc58 to six for STM0030 and STM0037 with an average of 4.4. In addition, a total of 77 unique patterns were observed for the 10 SSR markers. The polymorphic information content ranged from 0.477 to 0.802 with an average of 0.675 per locus. In this study, STM0037, STM1016, and STM1104 were found to be the best SSR markers to detect genetic differences between potato varieties. A minimum of two markers was required to distinguish between all 20 genotypes. Most importantly, this highly informative molecular tool confirmed that the developed potato varieties were genetically different from their respective maternal lines and potentially constituted new cultivars.https://doi.org/10.1002/pld3.140DNA fingerprintingpolymorphismpotato varieties identificationsingle sequence repeatSolanum tuberosum L.SSR markers |
| spellingShingle | Anne‐Sophie Tillault Dmytro P. Yevtushenko Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada Plant Direct DNA fingerprinting polymorphism potato varieties identification single sequence repeat Solanum tuberosum L. SSR markers |
| title | Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada |
| title_full | Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada |
| title_fullStr | Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada |
| title_full_unstemmed | Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada |
| title_short | Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada |
| title_sort | simple sequence repeat analysis of new potato varieties developed in alberta canada |
| topic | DNA fingerprinting polymorphism potato varieties identification single sequence repeat Solanum tuberosum L. SSR markers |
| url | https://doi.org/10.1002/pld3.140 |
| work_keys_str_mv | AT annesophietillault simplesequencerepeatanalysisofnewpotatovarietiesdevelopedinalbertacanada AT dmytropyevtushenko simplesequencerepeatanalysisofnewpotatovarietiesdevelopedinalbertacanada |