A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections
Abstract Background The clinical symptoms of invasive fungal infections (IFI) are nonspecific, and early clinical diagnosis is challenging, resulting in high mortality rates. This study reports the development of a novel aptamer-G-quadruplex/hemin self-assembling color system (AGSCS) based on (1 → 3...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-023-00570-6 |
| _version_ | 1797827623240335360 |
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| author | Ying Hua Feng Hu Xia Ren Yueling Xiong Jian Hu Fan Su Xiaolei Tang Yufeng Wen |
| author_facet | Ying Hua Feng Hu Xia Ren Yueling Xiong Jian Hu Fan Su Xiaolei Tang Yufeng Wen |
| author_sort | Ying Hua |
| collection | DOAJ |
| description | Abstract Background The clinical symptoms of invasive fungal infections (IFI) are nonspecific, and early clinical diagnosis is challenging, resulting in high mortality rates. This study reports the development of a novel aptamer-G-quadruplex/hemin self-assembling color system (AGSCS) based on (1 → 3)-β-D-glucans’ detection for rapid, specific and visual diagnosis of IFI. Methods We screened high affinity and specificity ssDNA aptamers binding to (1 → 3)-β-D-glucans, the main components of cell wall from Candida albicans via Systematic Evolution of Ligands by EXponential enrichment. Next, a comparison of diagnostic efficiency of AGSCS and the (1 → 3)-β-D-glucans assay (“G test”) with regard to predicting IFI in 198 clinical serum samples was done. Results Water-soluble (1 → 3)-β-D-glucans were successfully isolated from C. albicans ATCC 10,231 strain, and these low degree of polymerization glucans (< 1.7 kD) were targeted for aptamer screening with the complementary sequences of G-quadruplex. Six high affinity single stranded DNA aptamers (A1, A2, A3, A4, A5 and A6) were found. The linear detection range for (1 → 3)-β-D-glucans stretched from 1.6 pg/mL to 400 pg/mL on a microplate reader, and the detection limit was 3.125 pg/mL using naked eye observation. Using a microplate reader, the sensitivity and specificity of AGSCS for the diagnosis of IFI were 92.68% and 89.65%, respectively, which was higher than that of the G test. Conclusion This newly developed visual diagnostic method for detecting IFI showed promising results and is expected to be developed as a point-of-care testing kit to enable quick and cost effective diagnosis of IFI in the future. |
| first_indexed | 2024-04-09T12:51:17Z |
| format | Article |
| id | doaj.art-1bf3144c02184ecf817ea4d1a02f8e73 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-04-09T12:51:17Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-1bf3144c02184ecf817ea4d1a02f8e732023-05-14T11:10:57ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112023-05-0122111510.1186/s12941-023-00570-6A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infectionsYing Hua0Feng Hu1Xia Ren2Yueling Xiong3Jian Hu4Fan Su5Xiaolei Tang6Yufeng Wen7School of Nursing, Wannan Medical CollegeDepartment of Blood Transfusion, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College)School of Public Health, Wannan Medical CollegeCentre of Translational Medicine and Vascular Disease Research Center, The Second Affiliated Hospital of Wannan Medical CollegeSchool of Public Health, Wannan Medical CollegeSchool of Public Health, Wannan Medical CollegeCentre of Translational Medicine and Vascular Disease Research Center, The Second Affiliated Hospital of Wannan Medical CollegeSchool of Public Health, Wannan Medical CollegeAbstract Background The clinical symptoms of invasive fungal infections (IFI) are nonspecific, and early clinical diagnosis is challenging, resulting in high mortality rates. This study reports the development of a novel aptamer-G-quadruplex/hemin self-assembling color system (AGSCS) based on (1 → 3)-β-D-glucans’ detection for rapid, specific and visual diagnosis of IFI. Methods We screened high affinity and specificity ssDNA aptamers binding to (1 → 3)-β-D-glucans, the main components of cell wall from Candida albicans via Systematic Evolution of Ligands by EXponential enrichment. Next, a comparison of diagnostic efficiency of AGSCS and the (1 → 3)-β-D-glucans assay (“G test”) with regard to predicting IFI in 198 clinical serum samples was done. Results Water-soluble (1 → 3)-β-D-glucans were successfully isolated from C. albicans ATCC 10,231 strain, and these low degree of polymerization glucans (< 1.7 kD) were targeted for aptamer screening with the complementary sequences of G-quadruplex. Six high affinity single stranded DNA aptamers (A1, A2, A3, A4, A5 and A6) were found. The linear detection range for (1 → 3)-β-D-glucans stretched from 1.6 pg/mL to 400 pg/mL on a microplate reader, and the detection limit was 3.125 pg/mL using naked eye observation. Using a microplate reader, the sensitivity and specificity of AGSCS for the diagnosis of IFI were 92.68% and 89.65%, respectively, which was higher than that of the G test. Conclusion This newly developed visual diagnostic method for detecting IFI showed promising results and is expected to be developed as a point-of-care testing kit to enable quick and cost effective diagnosis of IFI in the future.https://doi.org/10.1186/s12941-023-00570-6Invasive fungal infections (IFI)AptamersSystematic Evolution of Ligands by EXponential enrichmentSelf-assemblingVisual diagnosisG-quadruplex |
| spellingShingle | Ying Hua Feng Hu Xia Ren Yueling Xiong Jian Hu Fan Su Xiaolei Tang Yufeng Wen A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections Annals of Clinical Microbiology and Antimicrobials Invasive fungal infections (IFI) Aptamers Systematic Evolution of Ligands by EXponential enrichment Self-assembling Visual diagnosis G-quadruplex |
| title | A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections |
| title_full | A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections |
| title_fullStr | A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections |
| title_full_unstemmed | A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections |
| title_short | A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections |
| title_sort | novel aptamer g quadruplex hemin self assembling color system rapid visual diagnosis of invasive fungal infections |
| topic | Invasive fungal infections (IFI) Aptamers Systematic Evolution of Ligands by EXponential enrichment Self-assembling Visual diagnosis G-quadruplex |
| url | https://doi.org/10.1186/s12941-023-00570-6 |
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