Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil
Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli are top-ranking. When these pathogens are associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, r...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2021.635206/full |
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| author | Melise Chaves Silveira Cláudio Marcos Rocha-de-Souza Ivson Cassiano de Oliveira Santos Leilane da Silva Pontes Thamirys Rachel Tavares e Oliveira Camila Bastos Tavares-Teixeira Nataly de Almeida Cossatis Natacha Ferreira Pereira Orlando Carlos da Conceição-Neto Bianca Santos da Costa Daiana Cristina Silva Rodrigues Rodolpho Mattos Albano Fabrício Alves Barbosa da Silva Elizabeth Andrade Marques Robson Souza Leão Ana Paula D'Alincourt Carvalho-Assef |
| author_facet | Melise Chaves Silveira Cláudio Marcos Rocha-de-Souza Ivson Cassiano de Oliveira Santos Leilane da Silva Pontes Thamirys Rachel Tavares e Oliveira Camila Bastos Tavares-Teixeira Nataly de Almeida Cossatis Natacha Ferreira Pereira Orlando Carlos da Conceição-Neto Bianca Santos da Costa Daiana Cristina Silva Rodrigues Rodolpho Mattos Albano Fabrício Alves Barbosa da Silva Elizabeth Andrade Marques Robson Souza Leão Ana Paula D'Alincourt Carvalho-Assef |
| author_sort | Melise Chaves Silveira |
| collection | DOAJ |
| description | Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli are top-ranking. When these pathogens are associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity and other molecular aspects of gram-negative bacilli (GNB) isolated from bloodstream infections in Brazil. It was possible to highlight international high-risk clones circulating in the Brazilian territory, such as CC258 for Klebsiella pneumoniae, ST79 for Acinetobacter baumannii and ST233 for Pseudomonas aeruginosa. Important associations can be made such as a negative correlation between CRISPR-Cas and K. pneumoniae CC258, while the genes blaTEM, blaKPC and blaCTX−M are highly associated with this clone. Specific relationships between A. baumannii clones and blaOXA−51 variants were also observed. All P. aeruginosa ST233 isolates showed the genes blaVIM and blaOXA486. In addition, some trends could be identified, where a new P. aeruginosa MDR clone (ST3079), a novel A. baumannii clonal profile circulating in Brazil (ST848), and important resistance associations in the form of blaVIM−2 and blaIMP−56 being found together in one ST233 strain, stand out. Such findings may help to develop approaches to deal with BSI and even other nosocomial infections caused by these important GNB. |
| first_indexed | 2024-12-16T15:11:16Z |
| format | Article |
| id | doaj.art-1bf46f2c4969457aa955c7c32e112a42 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-16T15:11:16Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-1bf46f2c4969457aa955c7c32e112a422022-12-21T22:26:57ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-03-01810.3389/fmed.2021.635206635206Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in BrazilMelise Chaves Silveira0Cláudio Marcos Rocha-de-Souza1Ivson Cassiano de Oliveira Santos2Leilane da Silva Pontes3Thamirys Rachel Tavares e Oliveira4Camila Bastos Tavares-Teixeira5Nataly de Almeida Cossatis6Natacha Ferreira Pereira7Orlando Carlos da Conceição-Neto8Bianca Santos da Costa9Daiana Cristina Silva Rodrigues10Rodolpho Mattos Albano11Fabrício Alves Barbosa da Silva12Elizabeth Andrade Marques13Robson Souza Leão14Ana Paula D'Alincourt Carvalho-Assef15Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Biologia Roberto de Alcântara Gome, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, BrazilPrograma de Computação Científica, Fundação Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, BrazilLaboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BrazilMultidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli are top-ranking. When these pathogens are associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity and other molecular aspects of gram-negative bacilli (GNB) isolated from bloodstream infections in Brazil. It was possible to highlight international high-risk clones circulating in the Brazilian territory, such as CC258 for Klebsiella pneumoniae, ST79 for Acinetobacter baumannii and ST233 for Pseudomonas aeruginosa. Important associations can be made such as a negative correlation between CRISPR-Cas and K. pneumoniae CC258, while the genes blaTEM, blaKPC and blaCTX−M are highly associated with this clone. Specific relationships between A. baumannii clones and blaOXA−51 variants were also observed. All P. aeruginosa ST233 isolates showed the genes blaVIM and blaOXA486. In addition, some trends could be identified, where a new P. aeruginosa MDR clone (ST3079), a novel A. baumannii clonal profile circulating in Brazil (ST848), and important resistance associations in the form of blaVIM−2 and blaIMP−56 being found together in one ST233 strain, stand out. Such findings may help to develop approaches to deal with BSI and even other nosocomial infections caused by these important GNB.https://www.frontiersin.org/articles/10.3389/fmed.2021.635206/fullmultidrug-resistancebloodstream infectionsgram-negative bacilliwhole-genome sequencingBrazilsurveillance |
| spellingShingle | Melise Chaves Silveira Cláudio Marcos Rocha-de-Souza Ivson Cassiano de Oliveira Santos Leilane da Silva Pontes Thamirys Rachel Tavares e Oliveira Camila Bastos Tavares-Teixeira Nataly de Almeida Cossatis Natacha Ferreira Pereira Orlando Carlos da Conceição-Neto Bianca Santos da Costa Daiana Cristina Silva Rodrigues Rodolpho Mattos Albano Fabrício Alves Barbosa da Silva Elizabeth Andrade Marques Robson Souza Leão Ana Paula D'Alincourt Carvalho-Assef Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil Frontiers in Medicine multidrug-resistance bloodstream infections gram-negative bacilli whole-genome sequencing Brazil surveillance |
| title | Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil |
| title_full | Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil |
| title_fullStr | Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil |
| title_full_unstemmed | Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil |
| title_short | Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil |
| title_sort | genetic basis of antimicrobial resistant gram negative bacteria isolated from bloodstream in brazil |
| topic | multidrug-resistance bloodstream infections gram-negative bacilli whole-genome sequencing Brazil surveillance |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2021.635206/full |
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