A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics
Highlights Co-cultivation was firstly carried out for the co-production of D-psicose and lipase. The carbon source glucose and fructose were fully utilized during co-culture. The yield of D- psicose was 11.70 g/L, and the conversion rate reached 69.54%. Self-induced expression of lipase was realized...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-08-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-021-00429-8 |
| _version_ | 1818645053181001728 |
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| author | Jun Zhang Wen Luo Zhiyuan Wang Xiaoyan Chen Pengmei Lv Jingliang Xu |
| author_facet | Jun Zhang Wen Luo Zhiyuan Wang Xiaoyan Chen Pengmei Lv Jingliang Xu |
| author_sort | Jun Zhang |
| collection | DOAJ |
| description | Highlights Co-cultivation was firstly carried out for the co-production of D-psicose and lipase. The carbon source glucose and fructose were fully utilized during co-culture. The yield of D- psicose was 11.70 g/L, and the conversion rate reached 69.54%. Self-induced expression of lipase was realized with enzyme activity of 16.03 U/mg. Metabolomics analyses were used to explore the co-culture effects of mixed strains. |
| first_indexed | 2024-12-17T00:24:37Z |
| format | Article |
| id | doaj.art-1bf50a5d596c47ca8eaf584105787037 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-17T00:24:37Z |
| publishDate | 2021-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-1bf50a5d596c47ca8eaf5841057870372022-12-21T22:10:30ZengSpringerOpenBioresources and Bioprocessing2197-43652021-08-018111810.1186/s40643-021-00429-8A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomicsJun Zhang0Wen Luo1Zhiyuan Wang2Xiaoyan Chen3Pengmei Lv4Jingliang Xu5Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyGuangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyGuangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyGuangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyGuangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyGuangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable EnergyHighlights Co-cultivation was firstly carried out for the co-production of D-psicose and lipase. The carbon source glucose and fructose were fully utilized during co-culture. The yield of D- psicose was 11.70 g/L, and the conversion rate reached 69.54%. Self-induced expression of lipase was realized with enzyme activity of 16.03 U/mg. Metabolomics analyses were used to explore the co-culture effects of mixed strains.https://doi.org/10.1186/s40643-021-00429-8D-PsicoseLipaseEscherichia coliBacillus subtilisCo-cultureMetabolomics |
| spellingShingle | Jun Zhang Wen Luo Zhiyuan Wang Xiaoyan Chen Pengmei Lv Jingliang Xu A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics Bioresources and Bioprocessing D-Psicose Lipase Escherichia coli Bacillus subtilis Co-culture Metabolomics |
| title | A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics |
| title_full | A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics |
| title_fullStr | A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics |
| title_full_unstemmed | A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics |
| title_short | A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics |
| title_sort | novel strategy for d psicose and lipase co production using a co culture system of engineered bacillus subtilis and escherichia coli and bioprocess analysis using metabolomics |
| topic | D-Psicose Lipase Escherichia coli Bacillus subtilis Co-culture Metabolomics |
| url | https://doi.org/10.1186/s40643-021-00429-8 |
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