The emergence of alternative 3' and 5' splice site exons from constitutive exons.
Alternative 3' and 5' splice site (ss) events constitute a significant part of all alternative splicing events. These events were also found to be related to several aberrant splicing diseases. However, only few of the characteristics that distinguish these events from alternative cassette...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-05-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC1876488?pdf=render |
| _version_ | 1818193778129764352 |
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| author | Eli Koren Galit Lev-Maor Gil Ast |
| author_facet | Eli Koren Galit Lev-Maor Gil Ast |
| author_sort | Eli Koren |
| collection | DOAJ |
| description | Alternative 3' and 5' splice site (ss) events constitute a significant part of all alternative splicing events. These events were also found to be related to several aberrant splicing diseases. However, only few of the characteristics that distinguish these events from alternative cassette exons are known currently. In this study, we compared the characteristics of constitutive exons, alternative cassette exons, and alternative 3'ss and 5'ss exons. The results revealed that alternative 3'ss and 5'ss exons are an intermediate state between constitutive and alternative cassette exons, where the constitutive side resembles constitutive exons, and the alternative side resembles alternative cassette exons. The results also show that alternative 3'ss and 5'ss exons exhibit low levels of symmetry (frame-preserving), similar to constitutive exons, whereas the sequence between the two alternative splice sites shows high symmetry levels, similar to alternative cassette exons. In addition, flanking intronic conservation analysis revealed that exons whose alternative splice sites are at least nine nucleotides apart show a high conservation level, indicating intronic participation in the regulation of their splicing, whereas exons whose alternative splice sites are fewer than nine nucleotides apart show a low conservation level. Further examination of these exons, spanning seven vertebrate species, suggests an evolutionary model in which the alternative state is a derivative of an ancestral constitutive exon, where a mutation inside the exon or along the flanking intron resulted in the creation of a new splice site that competes with the original one, leading to alternative splice site selection. This model was validated experimentally on four exons, showing that they indeed originated from constitutive exons that acquired a new competing splice site during evolution. |
| first_indexed | 2024-12-12T00:51:48Z |
| format | Article |
| id | doaj.art-1bf9c1bf73a548f8836659bea1368e6e |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-12T00:51:48Z |
| publishDate | 2007-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-1bf9c1bf73a548f8836659bea1368e6e2022-12-22T00:43:59ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582007-05-0135e9510.1371/journal.pcbi.0030095The emergence of alternative 3' and 5' splice site exons from constitutive exons.Eli KorenGalit Lev-MaorGil AstAlternative 3' and 5' splice site (ss) events constitute a significant part of all alternative splicing events. These events were also found to be related to several aberrant splicing diseases. However, only few of the characteristics that distinguish these events from alternative cassette exons are known currently. In this study, we compared the characteristics of constitutive exons, alternative cassette exons, and alternative 3'ss and 5'ss exons. The results revealed that alternative 3'ss and 5'ss exons are an intermediate state between constitutive and alternative cassette exons, where the constitutive side resembles constitutive exons, and the alternative side resembles alternative cassette exons. The results also show that alternative 3'ss and 5'ss exons exhibit low levels of symmetry (frame-preserving), similar to constitutive exons, whereas the sequence between the two alternative splice sites shows high symmetry levels, similar to alternative cassette exons. In addition, flanking intronic conservation analysis revealed that exons whose alternative splice sites are at least nine nucleotides apart show a high conservation level, indicating intronic participation in the regulation of their splicing, whereas exons whose alternative splice sites are fewer than nine nucleotides apart show a low conservation level. Further examination of these exons, spanning seven vertebrate species, suggests an evolutionary model in which the alternative state is a derivative of an ancestral constitutive exon, where a mutation inside the exon or along the flanking intron resulted in the creation of a new splice site that competes with the original one, leading to alternative splice site selection. This model was validated experimentally on four exons, showing that they indeed originated from constitutive exons that acquired a new competing splice site during evolution.http://europepmc.org/articles/PMC1876488?pdf=render |
| spellingShingle | Eli Koren Galit Lev-Maor Gil Ast The emergence of alternative 3' and 5' splice site exons from constitutive exons. PLoS Computational Biology |
| title | The emergence of alternative 3' and 5' splice site exons from constitutive exons. |
| title_full | The emergence of alternative 3' and 5' splice site exons from constitutive exons. |
| title_fullStr | The emergence of alternative 3' and 5' splice site exons from constitutive exons. |
| title_full_unstemmed | The emergence of alternative 3' and 5' splice site exons from constitutive exons. |
| title_short | The emergence of alternative 3' and 5' splice site exons from constitutive exons. |
| title_sort | emergence of alternative 3 and 5 splice site exons from constitutive exons |
| url | http://europepmc.org/articles/PMC1876488?pdf=render |
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