Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolat...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.961349/full |
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| author | Alaa Baazeem Saqer S. Alotaibi Luaay Kahtan Khalaf Uttam Kumar Madiha Zaynab Sarah Alharthi Hadeer Darwish Akram Alghamdi Suresh Kumar Jat Amal Al-Barty Bander Albogami Ahmed Noureldeen Balasubramani Ravindran Balasubramani Ravindran |
| author_facet | Alaa Baazeem Saqer S. Alotaibi Luaay Kahtan Khalaf Uttam Kumar Madiha Zaynab Sarah Alharthi Hadeer Darwish Akram Alghamdi Suresh Kumar Jat Amal Al-Barty Bander Albogami Ahmed Noureldeen Balasubramani Ravindran Balasubramani Ravindran |
| author_sort | Alaa Baazeem |
| collection | DOAJ |
| description | The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolated and molecularly characterized. Lysinibacillus xylanilyticus strain TU-2, Lysinibacillus xylanilyticus strain BN-13, Serratia liquefaciens strain TU-6, Stenotrophomonas tumulicola strain T5916-2-1b, and Pseudochrobactrum saccharolyticum strain CCUG are the strains. Pathogenicity tests demonstrated that bacterial cells were more toxic against the two aphid species than bacterial cell-free supernatants. S. tumulicola strain T5916-2-1b cells and filtrate were reported to have the strongest potential to kill A. punicae and A. illinoisensis individuals within 6 h after treatment, with 100% mortality of both insects 24 and 48 h after treatment. Based on the results of the study, it looked like endogenous Steinernema-associated EPB could be used directly as a biocontrol agent for A. punicae and A. illinoisensis. |
| first_indexed | 2024-04-11T19:42:27Z |
| format | Article |
| id | doaj.art-1bfa95523e1d49ea88656b763cd1128f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T19:42:27Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1bfa95523e1d49ea88656b763cd1128f2022-12-22T04:06:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.961349961349Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)Alaa Baazeem0Saqer S. Alotaibi1Luaay Kahtan Khalaf2Uttam Kumar3Madiha Zaynab4Sarah Alharthi5Hadeer Darwish6Akram Alghamdi7Suresh Kumar Jat8Amal Al-Barty9Bander Albogami10Ahmed Noureldeen11Balasubramani Ravindran12Balasubramani Ravindran13Department of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Plant Protection, College of Agricultural Engineering Science, University of Baghdad, Baghdad, IraqCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, ChinaDepartment of Chemistry, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Plant Protection, College of Horticulture and Forestry, Agriculture University, Kota, IndiaDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Environmental Energy and Engineering, Kyonggi University, Suwon, South KoreaDepartment of Medical Biotechnology and Integrative Physiology, Institute of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nadu, IndiaThe current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolated and molecularly characterized. Lysinibacillus xylanilyticus strain TU-2, Lysinibacillus xylanilyticus strain BN-13, Serratia liquefaciens strain TU-6, Stenotrophomonas tumulicola strain T5916-2-1b, and Pseudochrobactrum saccharolyticum strain CCUG are the strains. Pathogenicity tests demonstrated that bacterial cells were more toxic against the two aphid species than bacterial cell-free supernatants. S. tumulicola strain T5916-2-1b cells and filtrate were reported to have the strongest potential to kill A. punicae and A. illinoisensis individuals within 6 h after treatment, with 100% mortality of both insects 24 and 48 h after treatment. Based on the results of the study, it looked like endogenous Steinernema-associated EPB could be used directly as a biocontrol agent for A. punicae and A. illinoisensis.https://www.frontiersin.org/articles/10.3389/fmicb.2022.961349/fullentomopathogenic bacteriamolecular identificationAphis punicaeAphis illinoisensisbiological control |
| spellingShingle | Alaa Baazeem Saqer S. Alotaibi Luaay Kahtan Khalaf Uttam Kumar Madiha Zaynab Sarah Alharthi Hadeer Darwish Akram Alghamdi Suresh Kumar Jat Amal Al-Barty Bander Albogami Ahmed Noureldeen Balasubramani Ravindran Balasubramani Ravindran Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) Frontiers in Microbiology entomopathogenic bacteria molecular identification Aphis punicae Aphis illinoisensis biological control |
| title | Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) |
| title_full | Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) |
| title_fullStr | Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) |
| title_full_unstemmed | Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) |
| title_short | Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) |
| title_sort | identification and environment friendly biocontrol potential of five different bacteria against aphis punicae and aphis illinoisensis hemiptera aphididae |
| topic | entomopathogenic bacteria molecular identification Aphis punicae Aphis illinoisensis biological control |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.961349/full |
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