ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells
The zika virus (ZIKV) is a neurotropic virus that causes congenital abnormalities in babies when they are infected in utero. Some studies have reported these congenital abnormalities result from ZIKV attacking neural progenitor cells within the brain which differentiate into neurons, oligodendrocyte...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/7/771 |
| _version_ | 1827712951890477056 |
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| author | Kathleen Glover Kevin M. Coombs |
| author_facet | Kathleen Glover Kevin M. Coombs |
| author_sort | Kathleen Glover |
| collection | DOAJ |
| description | The zika virus (ZIKV) is a neurotropic virus that causes congenital abnormalities in babies when they are infected in utero. Some studies have reported these congenital abnormalities result from ZIKV attacking neural progenitor cells within the brain which differentiate into neurons, oligodendrocytes, and astrocytes. Each of these glial cells play important roles during development of the fetal brain. In addition to ZIKV-induced congenital abnormalities, infected patients experience gastrointestinal complications. There are presently no reports investigating the role of this virus at the proteomic level in gastrointestinal associated cells, so we conducted an in vitro proteomic study of ZIKV-induced changes in Caco-2, a colon-derived human cell line which is known to be permissive to ZIKV infection. We used SomaScan, a new aptamer-based proteomic tool to identify host proteins that are dysregulated during ZIKV infection at 12, 24, and 48 h post-infection. Bioinformatic analyses predicted that dysregulation of differentially-regulated host proteins results in various gastrointestinal diseases. Validation of the clinical relevance of these promising protein targets will add to the existing knowledge of ZIKV biology. These potential proteins may be useful targets towards the development of therapeutic interventions. |
| first_indexed | 2024-03-10T18:25:22Z |
| format | Article |
| id | doaj.art-1bfb70e9bae14ddcb92f6de69bddef49 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:25:22Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1bfb70e9bae14ddcb92f6de69bddef492023-11-20T07:02:45ZengMDPI AGViruses1999-49152020-07-0112777110.3390/v12070771ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal CellsKathleen Glover0Kevin M. Coombs1Department of Medical Microbiology and Infectious Diseases, Manitoba Centre for Proteomics & Systems Biology, Room 799, University of Manitoba, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, CanadaDepartment of Medical Microbiology and Infectious Diseases, Manitoba Centre for Proteomics & Systems Biology, Room 799, University of Manitoba, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, CanadaThe zika virus (ZIKV) is a neurotropic virus that causes congenital abnormalities in babies when they are infected in utero. Some studies have reported these congenital abnormalities result from ZIKV attacking neural progenitor cells within the brain which differentiate into neurons, oligodendrocytes, and astrocytes. Each of these glial cells play important roles during development of the fetal brain. In addition to ZIKV-induced congenital abnormalities, infected patients experience gastrointestinal complications. There are presently no reports investigating the role of this virus at the proteomic level in gastrointestinal associated cells, so we conducted an in vitro proteomic study of ZIKV-induced changes in Caco-2, a colon-derived human cell line which is known to be permissive to ZIKV infection. We used SomaScan, a new aptamer-based proteomic tool to identify host proteins that are dysregulated during ZIKV infection at 12, 24, and 48 h post-infection. Bioinformatic analyses predicted that dysregulation of differentially-regulated host proteins results in various gastrointestinal diseases. Validation of the clinical relevance of these promising protein targets will add to the existing knowledge of ZIKV biology. These potential proteins may be useful targets towards the development of therapeutic interventions.https://www.mdpi.com/1999-4915/12/7/771flavivirusdysregulated proteinsbioinformaticsgastrointestinal diseaseproteomicsaptamers |
| spellingShingle | Kathleen Glover Kevin M. Coombs ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells Viruses flavivirus dysregulated proteins bioinformatics gastrointestinal disease proteomics aptamers |
| title | ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells |
| title_full | ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells |
| title_fullStr | ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells |
| title_full_unstemmed | ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells |
| title_short | ZIKV Infection Induces DNA Damage Response and Alters the Proteome of Gastrointestinal Cells |
| title_sort | zikv infection induces dna damage response and alters the proteome of gastrointestinal cells |
| topic | flavivirus dysregulated proteins bioinformatics gastrointestinal disease proteomics aptamers |
| url | https://www.mdpi.com/1999-4915/12/7/771 |
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