Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models
Analyses of genetic variation allow understanding the origin, diversification and genetic resources of cultivated plants. Domesticated taxa and their wild relatives are ideal systems for studying genetic processes of plant domestication and their joint is important to evaluate the distribution of th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00400/full |
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| author | Guillermo Sánchez-de la Vega Gabriela Castellanos-Morales Gabriela Castellanos-Morales Gabriela Castellanos-Morales Niza Gámez Helena S. Hernández-Rosales Alejandra Vázquez-Lobo Alejandra Vázquez-Lobo Erika Aguirre-Planter Juan P. Jaramillo-Correa Salvador Montes-Hernández Rafael Lira-Saade Luis E. Eguiarte |
| author_facet | Guillermo Sánchez-de la Vega Gabriela Castellanos-Morales Gabriela Castellanos-Morales Gabriela Castellanos-Morales Niza Gámez Helena S. Hernández-Rosales Alejandra Vázquez-Lobo Alejandra Vázquez-Lobo Erika Aguirre-Planter Juan P. Jaramillo-Correa Salvador Montes-Hernández Rafael Lira-Saade Luis E. Eguiarte |
| author_sort | Guillermo Sánchez-de la Vega |
| collection | DOAJ |
| description | Analyses of genetic variation allow understanding the origin, diversification and genetic resources of cultivated plants. Domesticated taxa and their wild relatives are ideal systems for studying genetic processes of plant domestication and their joint is important to evaluate the distribution of their genetic resources. Such is the case of the domesticated subspecies C. argyrosperma ssp. argyrosperma, known in Mexico as calabaza pipiana, and its wild relative C. argyrosperma ssp. sororia. The main aim of this study was to use molecular data (microsatellites) to assess the levels of genetic variation and genetic differentiation within and among populations of domesticated argyrosperma across its distribution in Mexico in comparison to its wild relative, sororia, and to identify environmental suitability in previously proposed centers of domestication. We analyzed nine unlinked nuclear microsatellite loci to assess levels of diversity and distribution of genetic variation within and among populations in 440 individuals from 19 populations of cultivated landraces of argyrosperma and from six wild populations of sororia, in order to conduct a first systematic analysis of their genetic resources. We also used species distribution models (SDMs) for sororia to identify changes in this wild subspecies’ distribution from the Holocene (∼6,000 years ago) to the present, and to assess the presence of suitable environmental conditions in previously proposed domestication sites. Genetic variation was similar among subspecies (HE = 0.428 in sororia, and HE = 0.410 in argyrosperma). Nine argyrosperma populations showed significant levels of inbreeding. Both subspecies are well differentiated, and genetic differentiation (FST) among populations within each subspecies ranged from 0.152 to 0.652. Within argyrosperma we found three genetic groups (Northern Mexico, Yucatan Peninsula, including Michoacan and Veracruz, and Pacific coast plus Durango). We detected low levels of gene flow among populations at a regional scale (<0.01), except for the Yucatan Peninsula, and the northern portion of the Pacific Coast. Our analyses suggested that the Isthmus of Tehuantepec is an effective barrier isolating southern populations. Our SDM results indicate that environmental characteristics in the Balsas-Jalisco region, a potential center of domestication, were suitable for the presence of sororia during the Holocene. |
| first_indexed | 2024-12-21T23:57:34Z |
| format | Article |
| id | doaj.art-1bfc3f99be304be0baf63b0b5745e6eb |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T23:57:34Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-1bfc3f99be304be0baf63b0b5745e6eb2022-12-21T18:45:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00400303264Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution ModelsGuillermo Sánchez-de la Vega0Gabriela Castellanos-Morales1Gabriela Castellanos-Morales2Gabriela Castellanos-Morales3Niza Gámez4Helena S. Hernández-Rosales5Alejandra Vázquez-Lobo6Alejandra Vázquez-Lobo7Erika Aguirre-Planter8Juan P. Jaramillo-Correa9Salvador Montes-Hernández10Rafael Lira-Saade11Luis E. Eguiarte12Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoUnidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Conservación de la Biodiversidad, El Colegio de la Frontera Sur, Villahermosa, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoCentro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Cuernavaca, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoCampo Experimental Bajío, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Celaya, MexicoUnidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Mexico, MexicoDepartamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico, MexicoAnalyses of genetic variation allow understanding the origin, diversification and genetic resources of cultivated plants. Domesticated taxa and their wild relatives are ideal systems for studying genetic processes of plant domestication and their joint is important to evaluate the distribution of their genetic resources. Such is the case of the domesticated subspecies C. argyrosperma ssp. argyrosperma, known in Mexico as calabaza pipiana, and its wild relative C. argyrosperma ssp. sororia. The main aim of this study was to use molecular data (microsatellites) to assess the levels of genetic variation and genetic differentiation within and among populations of domesticated argyrosperma across its distribution in Mexico in comparison to its wild relative, sororia, and to identify environmental suitability in previously proposed centers of domestication. We analyzed nine unlinked nuclear microsatellite loci to assess levels of diversity and distribution of genetic variation within and among populations in 440 individuals from 19 populations of cultivated landraces of argyrosperma and from six wild populations of sororia, in order to conduct a first systematic analysis of their genetic resources. We also used species distribution models (SDMs) for sororia to identify changes in this wild subspecies’ distribution from the Holocene (∼6,000 years ago) to the present, and to assess the presence of suitable environmental conditions in previously proposed domestication sites. Genetic variation was similar among subspecies (HE = 0.428 in sororia, and HE = 0.410 in argyrosperma). Nine argyrosperma populations showed significant levels of inbreeding. Both subspecies are well differentiated, and genetic differentiation (FST) among populations within each subspecies ranged from 0.152 to 0.652. Within argyrosperma we found three genetic groups (Northern Mexico, Yucatan Peninsula, including Michoacan and Veracruz, and Pacific coast plus Durango). We detected low levels of gene flow among populations at a regional scale (<0.01), except for the Yucatan Peninsula, and the northern portion of the Pacific Coast. Our analyses suggested that the Isthmus of Tehuantepec is an effective barrier isolating southern populations. Our SDM results indicate that environmental characteristics in the Balsas-Jalisco region, a potential center of domestication, were suitable for the presence of sororia during the Holocene.http://journal.frontiersin.org/article/10.3389/fpls.2018.00400/fullCucurbitacultivated squashgenetic diversitygenetic structurenuclear microsatellitesspecies distribution models |
| spellingShingle | Guillermo Sánchez-de la Vega Gabriela Castellanos-Morales Gabriela Castellanos-Morales Gabriela Castellanos-Morales Niza Gámez Helena S. Hernández-Rosales Alejandra Vázquez-Lobo Alejandra Vázquez-Lobo Erika Aguirre-Planter Juan P. Jaramillo-Correa Salvador Montes-Hernández Rafael Lira-Saade Luis E. Eguiarte Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models Frontiers in Plant Science Cucurbita cultivated squash genetic diversity genetic structure nuclear microsatellites species distribution models |
| title | Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models |
| title_full | Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models |
| title_fullStr | Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models |
| title_full_unstemmed | Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models |
| title_short | Genetic Resources in the “Calabaza Pipiana” Squash (Cucurbita argyrosperma) in Mexico: Genetic Diversity, Genetic Differentiation and Distribution Models |
| title_sort | genetic resources in the calabaza pipiana squash cucurbita argyrosperma in mexico genetic diversity genetic differentiation and distribution models |
| topic | Cucurbita cultivated squash genetic diversity genetic structure nuclear microsatellites species distribution models |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00400/full |
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