Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens
Enterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent vaccine. There is a need to develop a cost eff ective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from field. Ind...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-06-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2813 |
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| author | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI |
| author_facet | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI |
| author_sort | Madeeha TARIQ |
| collection | DOAJ |
| description | Enterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent
vaccine. There is a need to develop a cost eff ective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from
field. Indigenously (Punjab, Pakistan) characterized Clostridium perfringens toxinotypes A (MW551947.1), B (MW332247.1) and D (MW332258.1)
(n=1 each) were used. These toxinotypes were used to produce higher amount of alpha, beta and epsilon toxin units under culture conditions.
Colony forming units (CFU) of each bacterium was determined through the standard plate count method and 106CFU/mL bacteria were used for
vaccine dose. Monovalent, bivalent and multivalent oil adjuvant and alum precipitate vaccines were prepared. Formulated vaccines were passed
the stability, sterility and safety test. Bacterin plus toxoid oil adjuvant vaccine produced higher (868.25±3.54 IU/mL) antibody titer at 28th day
post vaccination in rabbits and 100% protection was observed after challenge. Multivalent bacterin plus toxoid oil adjuvant vaccine was used in
field trials. Increased antibody response was detected after 4 months in sheep (1294.81±1.90 IU/mL) and goats (1091.85±2.51 IU/mL). During the
experimental and field trials commercial vaccine did not produced higher antibody titer. Multivalent bacterin plus toxoid oil adjuvant vaccine
proved as an excellent candidate for vaccination of animals against C. perfringens diseases, and it produced specific and efficient immune
response to be used in field. |
| first_indexed | 2024-03-13T04:39:02Z |
| format | Article |
| id | doaj.art-1bfc402e6c0e4054b3621554def9adf1 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:02Z |
| publishDate | 2021-06-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-1bfc402e6c0e4054b3621554def9adf12023-06-19T06:29:46ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-06-0127447548210.9775/kvfd.2021.257322813Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringensMadeeha TARIQ0Aftab Ahmad ANJUM1Ali Ahmad SHEIKH2Ali Raza AWAN3Muhammad Asad ALI4Mian Muhammad Khubaib SATTAR5Shahid HUSSAINTehreem ALI6Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANDepartment of Microbiology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANVaccine production section, INTERVAC (PVT) LTD, 18km Lahore Sheikhupura Road, Sheikhupura, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANEnterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent vaccine. There is a need to develop a cost eff ective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from field. Indigenously (Punjab, Pakistan) characterized Clostridium perfringens toxinotypes A (MW551947.1), B (MW332247.1) and D (MW332258.1) (n=1 each) were used. These toxinotypes were used to produce higher amount of alpha, beta and epsilon toxin units under culture conditions. Colony forming units (CFU) of each bacterium was determined through the standard plate count method and 106CFU/mL bacteria were used for vaccine dose. Monovalent, bivalent and multivalent oil adjuvant and alum precipitate vaccines were prepared. Formulated vaccines were passed the stability, sterility and safety test. Bacterin plus toxoid oil adjuvant vaccine produced higher (868.25±3.54 IU/mL) antibody titer at 28th day post vaccination in rabbits and 100% protection was observed after challenge. Multivalent bacterin plus toxoid oil adjuvant vaccine was used in field trials. Increased antibody response was detected after 4 months in sheep (1294.81±1.90 IU/mL) and goats (1091.85±2.51 IU/mL). During the experimental and field trials commercial vaccine did not produced higher antibody titer. Multivalent bacterin plus toxoid oil adjuvant vaccine proved as an excellent candidate for vaccination of animals against C. perfringens diseases, and it produced specific and efficient immune response to be used in field.https://vetdergikafkas.org/pdf.php?id=2813alphabacterinbetaepsilontoxoidvaccine |
| spellingShingle | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens Kafkas Universitesi Veteriner Fakültesi Dergisi alpha bacterin beta epsilon toxoid vaccine |
| title | Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| title_full | Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| title_fullStr | Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| title_full_unstemmed | Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| title_short | Preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| title_sort | preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| topic | alpha bacterin beta epsilon toxoid vaccine |
| url | https://vetdergikafkas.org/pdf.php?id=2813 |
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