Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress
Combretum micranthum (CM) leaves is commonly used as a traditional medication in West Africa to treat diabetes and renal illness. However, the effectiveness of CM extract in hypertensive nephropathy is not scientifically validated.The present experiment designed to illustrate the protecting ability...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646462200202X |
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| author | Mabozou Kpemissi Veeresh Prabhakar Veerapur Doddamavattur Shivalingaiah Suhas Tumbadi Adinarayanashetty Puneeth Rudrappa Nandeesh Sachidananda Vijayakumar Kwashie Eklu-Gadegbeku |
| author_facet | Mabozou Kpemissi Veeresh Prabhakar Veerapur Doddamavattur Shivalingaiah Suhas Tumbadi Adinarayanashetty Puneeth Rudrappa Nandeesh Sachidananda Vijayakumar Kwashie Eklu-Gadegbeku |
| author_sort | Mabozou Kpemissi |
| collection | DOAJ |
| description | Combretum micranthum (CM) leaves is commonly used as a traditional medication in West Africa to treat diabetes and renal illness. However, the effectiveness of CM extract in hypertensive nephropathy is not scientifically validated.The present experiment designed to illustrate the protecting ability of standardized extract of CM in L-NAME (N-ω-nitro-L-arginine methyl ester) intoxicated hypertensive nephropathic condition in rats and establish its mechanism. For induction, L-NAME (50 mg/Kg/day, p.o) was administered to male Wistar rats for six weeks to induce hypertensive nephropathy. Simultaneously, groups of rats were also treated with CM extract (200 mg/Kg/day and 400 mg/Kg/day) and Coversyl (5 mg/Kg/day) for the same period. Every week, the blood pressure, Electrocardiography and body weight of experimental animals were measured. At the end of the study, cardiac, liver and renal function tests were performed. Serum nitric oxide (NO), serum tumor necrosis factor-α (TNF-α), renal and cardiac oxidative stress markers were estimated. Furthermore, the extent of beneficial effect was re-confirmed by histopathological evaluation of heart and kidney tissues.Chronic L-NAME administration exhibited a reduction in serum nitric oxide (NO) along with the rise in blood pressure parameters. The deleterious hypertensive nephropathy was further confirmed by higher levels of oxidative stress, cardiac, renal and liver function markers and histopathological changes in myocardial and renal tissues. Daily oral administration of CM extract improved kidney function by reducing cardiac injury, renal damage, oxidative stress, inflammation, and histopathological changes caused by L-NAME hypertension.The present study provided the scientific confirmation about the traditional claims of Combretum micranthum. |
| first_indexed | 2024-04-12T13:50:06Z |
| format | Article |
| id | doaj.art-1bfc6dc07a2c4c23bff292327b51e088 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-12T13:50:06Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-1bfc6dc07a2c4c23bff292327b51e0882022-12-22T03:30:34ZengElsevierJournal of Functional Foods1756-46462022-07-0194105132Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stressMabozou Kpemissi0Veeresh Prabhakar Veerapur1Doddamavattur Shivalingaiah Suhas2Tumbadi Adinarayanashetty Puneeth3Rudrappa Nandeesh4Sachidananda Vijayakumar5Kwashie Eklu-Gadegbeku6Faculty of Sciences, University of Lomé, Togo; Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, India; Corresponding authors at: Laboratory of Physiology/Pharmacology, Department of Animal Physiology, Faculty of Sciences, University of Lomé, Togo (M. Kpemissi).Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, India; Corresponding authors at: Laboratory of Physiology/Pharmacology, Department of Animal Physiology, Faculty of Sciences, University of Lomé, Togo (M. Kpemissi).Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, IndiaSree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, IndiaSree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, IndiaSree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, IndiaFaculty of Sciences, University of Lomé, TogoCombretum micranthum (CM) leaves is commonly used as a traditional medication in West Africa to treat diabetes and renal illness. However, the effectiveness of CM extract in hypertensive nephropathy is not scientifically validated.The present experiment designed to illustrate the protecting ability of standardized extract of CM in L-NAME (N-ω-nitro-L-arginine methyl ester) intoxicated hypertensive nephropathic condition in rats and establish its mechanism. For induction, L-NAME (50 mg/Kg/day, p.o) was administered to male Wistar rats for six weeks to induce hypertensive nephropathy. Simultaneously, groups of rats were also treated with CM extract (200 mg/Kg/day and 400 mg/Kg/day) and Coversyl (5 mg/Kg/day) for the same period. Every week, the blood pressure, Electrocardiography and body weight of experimental animals were measured. At the end of the study, cardiac, liver and renal function tests were performed. Serum nitric oxide (NO), serum tumor necrosis factor-α (TNF-α), renal and cardiac oxidative stress markers were estimated. Furthermore, the extent of beneficial effect was re-confirmed by histopathological evaluation of heart and kidney tissues.Chronic L-NAME administration exhibited a reduction in serum nitric oxide (NO) along with the rise in blood pressure parameters. The deleterious hypertensive nephropathy was further confirmed by higher levels of oxidative stress, cardiac, renal and liver function markers and histopathological changes in myocardial and renal tissues. Daily oral administration of CM extract improved kidney function by reducing cardiac injury, renal damage, oxidative stress, inflammation, and histopathological changes caused by L-NAME hypertension.The present study provided the scientific confirmation about the traditional claims of Combretum micranthum.http://www.sciencedirect.com/science/article/pii/S175646462200202XKinkelibaHypertensionNephroprotectiveChronic kidney diseaseL-NAMENAG |
| spellingShingle | Mabozou Kpemissi Veeresh Prabhakar Veerapur Doddamavattur Shivalingaiah Suhas Tumbadi Adinarayanashetty Puneeth Rudrappa Nandeesh Sachidananda Vijayakumar Kwashie Eklu-Gadegbeku Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress Journal of Functional Foods Kinkeliba Hypertension Nephroprotective Chronic kidney disease L-NAME NAG |
| title | Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| title_full | Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| title_fullStr | Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| title_full_unstemmed | Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| title_short | Combretum micranthum G. Don protects hypertension induced by L-NAME by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| title_sort | combretum micranthum g don protects hypertension induced by l name by cardiovascular and renal remodelling through reversing inflammation and oxidative stress |
| topic | Kinkeliba Hypertension Nephroprotective Chronic kidney disease L-NAME NAG |
| url | http://www.sciencedirect.com/science/article/pii/S175646462200202X |
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