Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma
Background: The differentiation of Th1 and Th2 is strictly regulated by humoral and cellular factors. The imbalance between Th1 and Th2 is considered to be the pathogenesis of allergic and autoimmune disorders. It is important to elucidate the effect of environmental factors, such as temperature, on...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008-01-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015307966 |
| _version_ | 1811266385296228352 |
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| author | Takahiro Arai Hideo Kaneko Hidenori Ohnishi Eiko Matsui Toshiyuki Fukao Norio Kawamoto Kimiko Kasahara Naomi Kondo |
| author_facet | Takahiro Arai Hideo Kaneko Hidenori Ohnishi Eiko Matsui Toshiyuki Fukao Norio Kawamoto Kimiko Kasahara Naomi Kondo |
| author_sort | Takahiro Arai |
| collection | DOAJ |
| description | Background: The differentiation of Th1 and Th2 is strictly regulated by humoral and cellular factors. The imbalance between Th1 and Th2 is considered to be the pathogenesis of allergic and autoimmune disorders. It is important to elucidate the effect of environmental factors, such as temperature, on the expression of cytokines of Th1 and Th2.
Methods: We investigated the expression of IFN-gamma, IL-4, IL-5, IL-10 and IL-12 from LPS- or PHA-stimulated PBMCs at 30°C or 37°C using ELISA and Real-time PCR. We measured the change of NF-kappaB activity at 30°C or 37°C with LPS stimulation using the reporter gene assay.
Results: IFN-gamma production from LPS-stimulated PBMCs at 30°C was up-regulated compared with 37°C. IL-5 and IL-10 production from PHA-stimulated PBMCs at 30°C were down-regulated compared with 37°C. This augmented IFN-gamma production was caused by the up-regulation of IL-12 production from CD14+ blood monocytes. Both IL-12 mRNA and IL12 protein at 30°C were up-regulated compared with 37°C. NF-kappaB, the key molecule for the expression of IL-12, was also augmented at 30°C compared with 37°C.
Conclusions: Hypothermia up-regulated the expression of IL-12 and IFN-gamma due to the augmented NF-kappaB activity. It is suggested that hypothermia modifies the pattern of cytokine gene expression. |
| first_indexed | 2024-04-12T20:42:22Z |
| format | Article |
| id | doaj.art-1bfcd7a8257546bb84a34e2a521070f8 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-04-12T20:42:22Z |
| publishDate | 2008-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-1bfcd7a8257546bb84a34e2a521070f82022-12-22T03:17:23ZengElsevierAllergology International1323-89302008-01-0157433133810.2332/allergolint.O-08-532Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gammaTakahiro Arai0Hideo Kaneko1Hidenori Ohnishi2Eiko Matsui3Toshiyuki Fukao4Norio Kawamoto5Kimiko Kasahara6Naomi Kondo7Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan.Background: The differentiation of Th1 and Th2 is strictly regulated by humoral and cellular factors. The imbalance between Th1 and Th2 is considered to be the pathogenesis of allergic and autoimmune disorders. It is important to elucidate the effect of environmental factors, such as temperature, on the expression of cytokines of Th1 and Th2. Methods: We investigated the expression of IFN-gamma, IL-4, IL-5, IL-10 and IL-12 from LPS- or PHA-stimulated PBMCs at 30°C or 37°C using ELISA and Real-time PCR. We measured the change of NF-kappaB activity at 30°C or 37°C with LPS stimulation using the reporter gene assay. Results: IFN-gamma production from LPS-stimulated PBMCs at 30°C was up-regulated compared with 37°C. IL-5 and IL-10 production from PHA-stimulated PBMCs at 30°C were down-regulated compared with 37°C. This augmented IFN-gamma production was caused by the up-regulation of IL-12 production from CD14+ blood monocytes. Both IL-12 mRNA and IL12 protein at 30°C were up-regulated compared with 37°C. NF-kappaB, the key molecule for the expression of IL-12, was also augmented at 30°C compared with 37°C. Conclusions: Hypothermia up-regulated the expression of IL-12 and IFN-gamma due to the augmented NF-kappaB activity. It is suggested that hypothermia modifies the pattern of cytokine gene expression.http://www.sciencedirect.com/science/article/pii/S1323893015307966hypothermiaIFN-gammaIL-12lipopolysaccharideNF-kappaB |
| spellingShingle | Takahiro Arai Hideo Kaneko Hidenori Ohnishi Eiko Matsui Toshiyuki Fukao Norio Kawamoto Kimiko Kasahara Naomi Kondo Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma Allergology International hypothermia IFN-gamma IL-12 lipopolysaccharide NF-kappaB |
| title | Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma |
| title_full | Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma |
| title_fullStr | Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma |
| title_full_unstemmed | Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma |
| title_short | Hypothermia Augments NF-kappaB Activity and the Production of IL-12 and IFN-gamma |
| title_sort | hypothermia augments nf kappab activity and the production of il 12 and ifn gamma |
| topic | hypothermia IFN-gamma IL-12 lipopolysaccharide NF-kappaB |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015307966 |
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