Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4
Maltase is an economically valuable enzyme that is used to catalyze the hydrolytic process of maltose and yields d-glucose as a product. In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19304151 |
| _version_ | 1819110576724377600 |
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| author | Muhammad Asif Nawaz Sidra Pervez Muhsin Jamal Tour Jan Wali Khan Abdur Rauf Afsheen Aman Shah Ali Ul Qader |
| author_facet | Muhammad Asif Nawaz Sidra Pervez Muhsin Jamal Tour Jan Wali Khan Abdur Rauf Afsheen Aman Shah Ali Ul Qader |
| author_sort | Muhammad Asif Nawaz |
| collection | DOAJ |
| description | Maltase is an economically valuable enzyme that is used to catalyze the hydrolytic process of maltose and yields d-glucose as a product. In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum catalytic activity at 45 °C and pH-6.5 after 5.0 min. It presented greater stability within 0.1 M K2HPO4 buffer having pH-6.5 and showed 100 % activity even after 1.0 h. It retained 83.6 % and 45.0 % activity at 40 °C after 1.0 and 3.0 h, respectively. The enzyme retained 90.0 % activity at ˗20 °C even after 60 days. The molecular weight of enzyme was deduced to be 157.2 kDa as calculated using polyacrylamide gel electrophoresis (PAGE) and zymography. It was concluded that the characterized maltase has notable stability profile with reference to temperature, pH and other reaction conditions which anticipates its utilization in various starch and maltose hydrolyzing processes for the synthesis of glucose. Keywords: Maltase, Maltose, Bacillus licheniformis, Catalytic performance, Stability |
| first_indexed | 2024-12-22T03:43:55Z |
| format | Article |
| id | doaj.art-1bfe7bf8946e4d37b0b1350e94aa3c3b |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-22T03:43:55Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-1bfe7bf8946e4d37b0b1350e94aa3c3b2022-12-21T18:40:11ZengElsevierBiotechnology Reports2215-017X2019-12-0124Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4Muhammad Asif Nawaz0Sidra Pervez1Muhsin Jamal2Tour Jan3Wali Khan4Abdur Rauf5Afsheen Aman6Shah Ali Ul Qader7Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan; Corresponding author at: Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan.Department of Microbiology, Hazara University, Mansehra, KPK, PakistanDepartment of Microbiology, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, University of Malakand, Chakdara, Khyberpakhtunkhwa, PakistanDepartment of Zoology, University of Malakand, Chakdara, Khyberpakhtunkhwa, PakistanDepartment of Chemistry, University of Swabi, Khyberpakhtunkhwa, PakistanThe Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, PakistanDepartment of Biochemistry, University of Karachi, Karachi, 75270, PakistanMaltase is an economically valuable enzyme that is used to catalyze the hydrolytic process of maltose and yields d-glucose as a product. In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum catalytic activity at 45 °C and pH-6.5 after 5.0 min. It presented greater stability within 0.1 M K2HPO4 buffer having pH-6.5 and showed 100 % activity even after 1.0 h. It retained 83.6 % and 45.0 % activity at 40 °C after 1.0 and 3.0 h, respectively. The enzyme retained 90.0 % activity at ˗20 °C even after 60 days. The molecular weight of enzyme was deduced to be 157.2 kDa as calculated using polyacrylamide gel electrophoresis (PAGE) and zymography. It was concluded that the characterized maltase has notable stability profile with reference to temperature, pH and other reaction conditions which anticipates its utilization in various starch and maltose hydrolyzing processes for the synthesis of glucose. Keywords: Maltase, Maltose, Bacillus licheniformis, Catalytic performance, Stabilityhttp://www.sciencedirect.com/science/article/pii/S2215017X19304151 |
| spellingShingle | Muhammad Asif Nawaz Sidra Pervez Muhsin Jamal Tour Jan Wali Khan Abdur Rauf Afsheen Aman Shah Ali Ul Qader Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 Biotechnology Reports |
| title | Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 |
| title_full | Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 |
| title_fullStr | Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 |
| title_full_unstemmed | Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 |
| title_short | Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4 |
| title_sort | maltose deterioration approach catalytic behavior optimization and stability profile of maltase from bacillus licheniformis kibge ib4 |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X19304151 |
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