| Shrnutí: | <i>Haemagogus (Haemagogus) janthinomys</i> (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub>1</sub> generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub>3</sub> generation <i>Hg. janthinomys</i> eggs for the first time. A total of 62.8% (1562/2486) F<sub>1</sub> generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub>2</sub> generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub>2</sub> mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i>Hg. janthinomys</i> past the F<sub>3</sub> generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
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