Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field
Agaves are plants native to North America that sustain life in arid and semiarid ecosystems. Previous studies revealed that cultivated plants of Agave tequilana had lower microbial diversity and functionality than wild Agave species. Here, we tested if synthetic communities (syncoms), based on micro...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-12-01
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| Series: | Phytobiomes Journal |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-01-23-0001-R |
| _version_ | 1797389728558874624 |
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| author | Víctor M. Flores-Núñez David A. Camarena-Pozos J. Daniel Chávez-González Raúl Alcalde-Vázquez M. Nélida Vázquez-Sánchez Alan G. Hernández-Melgar Jorge Xool-Tamayo Aldo Moreno-Ulloa Laila P. Partida Martínez |
| author_facet | Víctor M. Flores-Núñez David A. Camarena-Pozos J. Daniel Chávez-González Raúl Alcalde-Vázquez M. Nélida Vázquez-Sánchez Alan G. Hernández-Melgar Jorge Xool-Tamayo Aldo Moreno-Ulloa Laila P. Partida Martínez |
| author_sort | Víctor M. Flores-Núñez |
| collection | DOAJ |
| description | Agaves are plants native to North America that sustain life in arid and semiarid ecosystems. Previous studies revealed that cultivated plants of Agave tequilana had lower microbial diversity and functionality than wild Agave species. Here, we tested if synthetic communities (syncoms), based on microbial hubs or taxa with enriched microbial functions, could increase microbial diversity, plant health, and productivity in A. tequilana. We applied 10 syncoms to the phyllospheres of 6-month-old plants of A. tequilana in the field and monitored their development for 2 years. Amplicon sequencing of 16S-rRNA-V4 and ITS2 revealed that the inoculated syncoms played a negligible or minor role in the assembly of the prokaryotic and eukaryotic phyllospheric communities associated with A. tequilana, respectively. However, syncoms based on microbial hubs, particularly those observed in the phyllosphere associated with wild A. salmiana (PFCS), promoted microbial communities with higher alpha diversity. Some of these syncom-derived phyllospheric communities consumed a greater variety of carbon sources; had more complex co-occurrence networks; and increased the content of sugars (oBrix, a measure of productivity in agaves) in the stem and changed the leaf metabolome. Our work demonstrates that the application of syncoms formulated based on predicted microbe–microbe interactions and metagenomic analyses of microbial communities in cultivated and wild plant species represents an effective tool to improve the sustainability and productivity of crops in arid ecosystems. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-03-08T23:01:11Z |
| format | Article |
| id | doaj.art-1c0062c744384a6099c64bbdbefa1205 |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-03-08T23:01:11Z |
| publishDate | 2023-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-1c0062c744384a6099c64bbdbefa12052023-12-15T20:15:02ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062023-12-017443544810.1094/PBIOMES-01-23-0001-RSynthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the FieldVíctor M. Flores-Núñez0David A. Camarena-Pozos1J. Daniel Chávez-González2Raúl Alcalde-Vázquez3M. Nélida Vázquez-Sánchez4Alan G. Hernández-Melgar5Jorge Xool-Tamayo6Aldo Moreno-Ulloa7Laila P. Partida Martínez8Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoDepartamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoDepartamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoDepartamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoDepartamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoDepartamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California (CICESE), Ensenada 22860, MéxicoDepartamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California (CICESE), Ensenada 22860, MéxicoDepartamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California (CICESE), Ensenada 22860, MéxicoDepartamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato 36824, MéxicoAgaves are plants native to North America that sustain life in arid and semiarid ecosystems. Previous studies revealed that cultivated plants of Agave tequilana had lower microbial diversity and functionality than wild Agave species. Here, we tested if synthetic communities (syncoms), based on microbial hubs or taxa with enriched microbial functions, could increase microbial diversity, plant health, and productivity in A. tequilana. We applied 10 syncoms to the phyllospheres of 6-month-old plants of A. tequilana in the field and monitored their development for 2 years. Amplicon sequencing of 16S-rRNA-V4 and ITS2 revealed that the inoculated syncoms played a negligible or minor role in the assembly of the prokaryotic and eukaryotic phyllospheric communities associated with A. tequilana, respectively. However, syncoms based on microbial hubs, particularly those observed in the phyllosphere associated with wild A. salmiana (PFCS), promoted microbial communities with higher alpha diversity. Some of these syncom-derived phyllospheric communities consumed a greater variety of carbon sources; had more complex co-occurrence networks; and increased the content of sugars (oBrix, a measure of productivity in agaves) in the stem and changed the leaf metabolome. Our work demonstrates that the application of syncoms formulated based on predicted microbe–microbe interactions and metagenomic analyses of microbial communities in cultivated and wild plant species represents an effective tool to improve the sustainability and productivity of crops in arid ecosystems. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-01-23-0001-RAgave tequilanaagriculture microbiomephyllospheresyncoms |
| spellingShingle | Víctor M. Flores-Núñez David A. Camarena-Pozos J. Daniel Chávez-González Raúl Alcalde-Vázquez M. Nélida Vázquez-Sánchez Alan G. Hernández-Melgar Jorge Xool-Tamayo Aldo Moreno-Ulloa Laila P. Partida Martínez Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field Phytobiomes Journal Agave tequilana agriculture microbiome phyllosphere syncoms |
| title | Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field |
| title_full | Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field |
| title_fullStr | Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field |
| title_full_unstemmed | Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field |
| title_short | Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field |
| title_sort | synthetic communities increase microbial diversity and productivity of agave tequilana plants in the field |
| topic | Agave tequilana agriculture microbiome phyllosphere syncoms |
| url | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-01-23-0001-R |
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